Investigation of Genipin Cross-Linked Microcapsule for Oral Delivery of Live Bacterial Cells and Other Biotherapeutics: Preparation and In Vitro Analysis in Simulated Human Gastrointestinal Model
Oral therapy utilizing engineered microorganisms has shown promise in the treatment of many diseases. By microencapsulation, viable cells can overcome the harsh gastrointestinal (GI) environment and secrete needed therapeutics into the gut. These engineered cells should be encased without escaping i...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2010-01-01
|
| Series: | International Journal of Polymer Science |
| Online Access: | http://dx.doi.org/10.1155/2010/985137 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832558278547079168 |
|---|---|
| author | Hongmei Chen Wei Ouyang Christopher Martoni Fatemeh Afkhami Bisi Lawuyi Trisna Lim Satya Prakash |
| author_facet | Hongmei Chen Wei Ouyang Christopher Martoni Fatemeh Afkhami Bisi Lawuyi Trisna Lim Satya Prakash |
| author_sort | Hongmei Chen |
| collection | DOAJ |
| description | Oral therapy utilizing engineered microorganisms has shown promise in the treatment of many diseases. By microencapsulation, viable cells can overcome the harsh gastrointestinal (GI) environment and secrete needed therapeutics into the gut. These engineered cells should be encased without escaping into the GI tract for safety concerns, thus robust microcapsule membrane is requisite. This paper examined the GI performance of a novel microcapsule membrane using a dynamic simulated human GI model. Results showed that the genipin cross-linked alginate-chitosan (GCAC) microcapsules possessed strong resistance to structural disintegration in the simulated GI environment. Leakage of encapsulated high molecular weight dextran, a model material to be protected during the simulated GI transit, was negligible over 72 h of exposure, in contrast to considerable leakage of dextran from the non-cross-linked counterparts. These microcapsules did not alter the microflora and enzymatic activities in the simulated human colonic media. This study suggested the potential of the GCAC microcapsules for oral delivery of live microorganisms and other biotherapeutics. |
| format | Article |
| id | doaj-art-d67ffec5dd114cb0af040b214de06cee |
| institution | Kabale University |
| issn | 1687-9422 1687-9430 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Polymer Science |
| spelling | doaj-art-d67ffec5dd114cb0af040b214de06cee2025-02-03T01:32:55ZengWileyInternational Journal of Polymer Science1687-94221687-94302010-01-01201010.1155/2010/985137985137Investigation of Genipin Cross-Linked Microcapsule for Oral Delivery of Live Bacterial Cells and Other Biotherapeutics: Preparation and In Vitro Analysis in Simulated Human Gastrointestinal ModelHongmei Chen0Wei Ouyang1Christopher Martoni2Fatemeh Afkhami3Bisi Lawuyi4Trisna Lim5Satya Prakash6Department of Biomedical Engineering and Physiology, Biomedical Technology and Cell Therapy Research Laboratory, Artificial Cells and Organs Research Centre, Faculty of Medicine, McGill University, Montreal, Qc, H3A 2B4, CanadaDepartment of Biomedical Engineering and Physiology, Biomedical Technology and Cell Therapy Research Laboratory, Artificial Cells and Organs Research Centre, Faculty of Medicine, McGill University, Montreal, Qc, H3A 2B4, CanadaDepartment of Biomedical Engineering and Physiology, Biomedical Technology and Cell Therapy Research Laboratory, Artificial Cells and Organs Research Centre, Faculty of Medicine, McGill University, Montreal, Qc, H3A 2B4, CanadaDepartment of Biomedical Engineering and Physiology, Biomedical Technology and Cell Therapy Research Laboratory, Artificial Cells and Organs Research Centre, Faculty of Medicine, McGill University, Montreal, Qc, H3A 2B4, CanadaDepartment of Biomedical Engineering and Physiology, Biomedical Technology and Cell Therapy Research Laboratory, Artificial Cells and Organs Research Centre, Faculty of Medicine, McGill University, Montreal, Qc, H3A 2B4, CanadaDepartment of Biomedical Engineering and Physiology, Biomedical Technology and Cell Therapy Research Laboratory, Artificial Cells and Organs Research Centre, Faculty of Medicine, McGill University, Montreal, Qc, H3A 2B4, CanadaDepartment of Biomedical Engineering and Physiology, Biomedical Technology and Cell Therapy Research Laboratory, Artificial Cells and Organs Research Centre, Faculty of Medicine, McGill University, Montreal, Qc, H3A 2B4, CanadaOral therapy utilizing engineered microorganisms has shown promise in the treatment of many diseases. By microencapsulation, viable cells can overcome the harsh gastrointestinal (GI) environment and secrete needed therapeutics into the gut. These engineered cells should be encased without escaping into the GI tract for safety concerns, thus robust microcapsule membrane is requisite. This paper examined the GI performance of a novel microcapsule membrane using a dynamic simulated human GI model. Results showed that the genipin cross-linked alginate-chitosan (GCAC) microcapsules possessed strong resistance to structural disintegration in the simulated GI environment. Leakage of encapsulated high molecular weight dextran, a model material to be protected during the simulated GI transit, was negligible over 72 h of exposure, in contrast to considerable leakage of dextran from the non-cross-linked counterparts. These microcapsules did not alter the microflora and enzymatic activities in the simulated human colonic media. This study suggested the potential of the GCAC microcapsules for oral delivery of live microorganisms and other biotherapeutics.http://dx.doi.org/10.1155/2010/985137 |
| spellingShingle | Hongmei Chen Wei Ouyang Christopher Martoni Fatemeh Afkhami Bisi Lawuyi Trisna Lim Satya Prakash Investigation of Genipin Cross-Linked Microcapsule for Oral Delivery of Live Bacterial Cells and Other Biotherapeutics: Preparation and In Vitro Analysis in Simulated Human Gastrointestinal Model International Journal of Polymer Science |
| title | Investigation of Genipin Cross-Linked Microcapsule for Oral Delivery of Live Bacterial Cells and Other Biotherapeutics: Preparation and In Vitro Analysis in Simulated Human Gastrointestinal Model |
| title_full | Investigation of Genipin Cross-Linked Microcapsule for Oral Delivery of Live Bacterial Cells and Other Biotherapeutics: Preparation and In Vitro Analysis in Simulated Human Gastrointestinal Model |
| title_fullStr | Investigation of Genipin Cross-Linked Microcapsule for Oral Delivery of Live Bacterial Cells and Other Biotherapeutics: Preparation and In Vitro Analysis in Simulated Human Gastrointestinal Model |
| title_full_unstemmed | Investigation of Genipin Cross-Linked Microcapsule for Oral Delivery of Live Bacterial Cells and Other Biotherapeutics: Preparation and In Vitro Analysis in Simulated Human Gastrointestinal Model |
| title_short | Investigation of Genipin Cross-Linked Microcapsule for Oral Delivery of Live Bacterial Cells and Other Biotherapeutics: Preparation and In Vitro Analysis in Simulated Human Gastrointestinal Model |
| title_sort | investigation of genipin cross linked microcapsule for oral delivery of live bacterial cells and other biotherapeutics preparation and in vitro analysis in simulated human gastrointestinal model |
| url | http://dx.doi.org/10.1155/2010/985137 |
| work_keys_str_mv | AT hongmeichen investigationofgenipincrosslinkedmicrocapsulefororaldeliveryoflivebacterialcellsandotherbiotherapeuticspreparationandinvitroanalysisinsimulatedhumangastrointestinalmodel AT weiouyang investigationofgenipincrosslinkedmicrocapsulefororaldeliveryoflivebacterialcellsandotherbiotherapeuticspreparationandinvitroanalysisinsimulatedhumangastrointestinalmodel AT christophermartoni investigationofgenipincrosslinkedmicrocapsulefororaldeliveryoflivebacterialcellsandotherbiotherapeuticspreparationandinvitroanalysisinsimulatedhumangastrointestinalmodel AT fatemehafkhami investigationofgenipincrosslinkedmicrocapsulefororaldeliveryoflivebacterialcellsandotherbiotherapeuticspreparationandinvitroanalysisinsimulatedhumangastrointestinalmodel AT bisilawuyi investigationofgenipincrosslinkedmicrocapsulefororaldeliveryoflivebacterialcellsandotherbiotherapeuticspreparationandinvitroanalysisinsimulatedhumangastrointestinalmodel AT trisnalim investigationofgenipincrosslinkedmicrocapsulefororaldeliveryoflivebacterialcellsandotherbiotherapeuticspreparationandinvitroanalysisinsimulatedhumangastrointestinalmodel AT satyaprakash investigationofgenipincrosslinkedmicrocapsulefororaldeliveryoflivebacterialcellsandotherbiotherapeuticspreparationandinvitroanalysisinsimulatedhumangastrointestinalmodel |