Early RSV infection aggravates asthma-related Th2 responses by increasing the number of CD4<sup>+</sup> TRM cells through upregulation of PLZF
Respiratory syncytial virus (RSV) infection is correlated with the chronic pathogenesis and exacerbation of asthma. However, the mechanism remains unclear. In this study, acute and memory (Mem) asthma models with early RSV infection are established to explore the persistence of the effects of RSV in...
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| Format: | Article |
| Language: | English |
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China Science Publishing & Media Ltd.
2024-12-01
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| Series: | Acta Biochimica et Biophysica Sinica |
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| Online Access: | https://www.sciengine.com/doi/10.3724/abbs.2024220 |
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| author | Zhang Meng Sha Jiafeng Li Na Feng Jingjing Shi Tianyun Yu Yunxia Ren Xiaoting Mei Zhoufang Jie Zhijun |
| author_facet | Zhang Meng Sha Jiafeng Li Na Feng Jingjing Shi Tianyun Yu Yunxia Ren Xiaoting Mei Zhoufang Jie Zhijun |
| author_sort | Zhang Meng |
| collection | DOAJ |
| description | Respiratory syncytial virus (RSV) infection is correlated with the chronic pathogenesis and exacerbation of asthma. However, the mechanism remains unclear. In this study, acute and memory (Mem) asthma models with early RSV infection are established to explore the persistence of the effects of RSV infection on asthma. Intravascular injection of an anti-CD45 antibody is performed to define CD4<sup>+</sup> TRM cells accurately. RSV infection has a sustained impact on asthma exacerbation for at least six weeks, with high Th2 cytokine secretion in lung tissue instead of IgE response-related B cells. CD45<sup>–</sup>CD4<sup>+</sup> TRM cells are positively correlated with RSV-related asthma exacerbation and severe airway inflammation. Mechanistically, overexpression of the transcription factor PLZF in vitro increases the number of CD4<sup>+</sup> TRM cells, and conditional knockout of Zbtb16 (encoding PLZF) can decrease the number of CD4<sup>+</sup> TRM cells to aggravate allergic inflammation and reduce Th2 responses. This study provides evidence for potential combined strategies that might benefit asthma patients. |
| format | Article |
| id | doaj-art-d67734fe51b24ee6bf38f2477e2c5a7e |
| institution | Kabale University |
| issn | 1672-9145 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | China Science Publishing & Media Ltd. |
| record_format | Article |
| series | Acta Biochimica et Biophysica Sinica |
| spelling | doaj-art-d67734fe51b24ee6bf38f2477e2c5a7e2025-08-20T03:28:25ZengChina Science Publishing & Media Ltd.Acta Biochimica et Biophysica Sinica1672-91452024-12-015794195410.3724/abbs.202422020d259ccEarly RSV infection aggravates asthma-related Th2 responses by increasing the number of CD4<sup>+</sup> TRM cells through upregulation of PLZFZhang Meng0Sha Jiafeng1Li Na2Feng Jingjing3Shi Tianyun4Yu Yunxia5Ren Xiaoting6Mei Zhoufang7Jie Zhijun8["Department of Pulmonary and Critical Care Medicine, Shanghai Fifth People’s Hospital, Fudan University, Shanghai 200240, China"]["Department of Pulmonary and Critical Care Medicine, Shanghai Fifth People’s Hospital, Fudan University, Shanghai 200240, China"]["Department of Medicine, Respiratory, Emergency and Intensive Care Medicine, The Affiliated Dushu Lake Hospital of Soochow University, Suzhou 215128, China"]["Department of Pulmonary and Critical Care Medicine, Shanghai Fifth People’s Hospital, Fudan University, Shanghai 200240, China"]["Department of Pulmonary and Critical Care Medicine, Shanghai Fifth People’s Hospital, Fudan University, Shanghai 200240, China"]["Department of Pulmonary and Critical Care Medicine, Shanghai Fifth People’s Hospital, Fudan University, Shanghai 200240, China"]["Department of Pulmonary and Critical Care Medicine, Shanghai Fifth People’s Hospital, Fudan University, Shanghai 200240, China"]["Department of Pulmonary and Critical Care Medicine, Shanghai Fifth People’s Hospital, Fudan University, Shanghai 200240, China"]["Department of Pulmonary and Critical Care Medicine, Shanghai Fifth People’s Hospital, Fudan University, Shanghai 200240, China","Center of Community-Based Health Research, Fudan University, Shanghai 200032, China"]Respiratory syncytial virus (RSV) infection is correlated with the chronic pathogenesis and exacerbation of asthma. However, the mechanism remains unclear. In this study, acute and memory (Mem) asthma models with early RSV infection are established to explore the persistence of the effects of RSV infection on asthma. Intravascular injection of an anti-CD45 antibody is performed to define CD4<sup>+</sup> TRM cells accurately. RSV infection has a sustained impact on asthma exacerbation for at least six weeks, with high Th2 cytokine secretion in lung tissue instead of IgE response-related B cells. CD45<sup>–</sup>CD4<sup>+</sup> TRM cells are positively correlated with RSV-related asthma exacerbation and severe airway inflammation. Mechanistically, overexpression of the transcription factor PLZF in vitro increases the number of CD4<sup>+</sup> TRM cells, and conditional knockout of Zbtb16 (encoding PLZF) can decrease the number of CD4<sup>+</sup> TRM cells to aggravate allergic inflammation and reduce Th2 responses. This study provides evidence for potential combined strategies that might benefit asthma patients.https://www.sciengine.com/doi/10.3724/abbs.2024220asthmarespiratory syncytial virus infectionPLZFtissue-resident memory T cell |
| spellingShingle | Zhang Meng Sha Jiafeng Li Na Feng Jingjing Shi Tianyun Yu Yunxia Ren Xiaoting Mei Zhoufang Jie Zhijun Early RSV infection aggravates asthma-related Th2 responses by increasing the number of CD4<sup>+</sup> TRM cells through upregulation of PLZF Acta Biochimica et Biophysica Sinica asthma respiratory syncytial virus infection PLZF tissue-resident memory T cell |
| title | Early RSV infection aggravates asthma-related Th2 responses by increasing the number of CD4<sup>+</sup> TRM cells through upregulation of PLZF |
| title_full | Early RSV infection aggravates asthma-related Th2 responses by increasing the number of CD4<sup>+</sup> TRM cells through upregulation of PLZF |
| title_fullStr | Early RSV infection aggravates asthma-related Th2 responses by increasing the number of CD4<sup>+</sup> TRM cells through upregulation of PLZF |
| title_full_unstemmed | Early RSV infection aggravates asthma-related Th2 responses by increasing the number of CD4<sup>+</sup> TRM cells through upregulation of PLZF |
| title_short | Early RSV infection aggravates asthma-related Th2 responses by increasing the number of CD4<sup>+</sup> TRM cells through upregulation of PLZF |
| title_sort | early rsv infection aggravates asthma related th2 responses by increasing the number of cd4 sup sup trm cells through upregulation of plzf |
| topic | asthma respiratory syncytial virus infection PLZF tissue-resident memory T cell |
| url | https://www.sciengine.com/doi/10.3724/abbs.2024220 |
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