Muscarinic ACh Receptors Contribute to Aversive Olfactory Learning in Drosophila
The most studied form of associative learning in Drosophila consists in pairing an odorant, the conditioned stimulus (CS), with an unconditioned stimulus (US). The timely arrival of the CS and US information to a specific Drosophila brain association region, the mushroom bodies (MB), can induce new...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2015/658918 |
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| author | Bryon Silva Claudia Molina-Fernández María Beatriz Ugalde Eduardo I. Tognarelli Cristian Angel Jorge M. Campusano |
| author_facet | Bryon Silva Claudia Molina-Fernández María Beatriz Ugalde Eduardo I. Tognarelli Cristian Angel Jorge M. Campusano |
| author_sort | Bryon Silva |
| collection | DOAJ |
| description | The most studied form of associative learning in Drosophila consists in pairing an odorant, the conditioned stimulus (CS), with an unconditioned stimulus (US). The timely arrival of the CS and US information to a specific Drosophila brain association region, the mushroom bodies (MB), can induce new olfactory memories. Thus, the MB is considered a coincidence detector. It has been shown that olfactory information is conveyed to the MB through cholinergic inputs that activate acetylcholine (ACh) receptors, while the US is encoded by biogenic amine (BA) systems. In recent years, we have advanced our understanding on the specific neural BA pathways and receptors involved in olfactory learning and memory. However, little information exists on the contribution of cholinergic receptors to this process. Here we evaluate for the first time the proposition that, as in mammals, muscarinic ACh receptors (mAChRs) contribute to memory formation in Drosophila. Our results show that pharmacological and genetic blockade of mAChRs in MB disrupts olfactory aversive memory in larvae. This effect is not explained by an alteration in the ability of animals to respond to odorants or to execute motor programs. These results show that mAChRs in MB contribute to generating olfactory memories in Drosophila. |
| format | Article |
| id | doaj-art-d66b2bd45cf14315a33c95b3c490f4b8 |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-d66b2bd45cf14315a33c95b3c490f4b82025-02-03T01:03:02ZengWileyNeural Plasticity2090-59041687-54432015-01-01201510.1155/2015/658918658918Muscarinic ACh Receptors Contribute to Aversive Olfactory Learning in DrosophilaBryon Silva0Claudia Molina-Fernández1María Beatriz Ugalde2Eduardo I. Tognarelli3Cristian Angel4Jorge M. Campusano5Laboratorio Neurogenética de la Conducta, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Alameda 340, 8331150 Santiago, ChileLaboratorio Neurogenética de la Conducta, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Alameda 340, 8331150 Santiago, ChileLaboratorio Neurogenética de la Conducta, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Alameda 340, 8331150 Santiago, ChileLaboratorio Neurogenética de la Conducta, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Alameda 340, 8331150 Santiago, ChileLaboratorio Neurogenética de la Conducta, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Alameda 340, 8331150 Santiago, ChileLaboratorio Neurogenética de la Conducta, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Alameda 340, 8331150 Santiago, ChileThe most studied form of associative learning in Drosophila consists in pairing an odorant, the conditioned stimulus (CS), with an unconditioned stimulus (US). The timely arrival of the CS and US information to a specific Drosophila brain association region, the mushroom bodies (MB), can induce new olfactory memories. Thus, the MB is considered a coincidence detector. It has been shown that olfactory information is conveyed to the MB through cholinergic inputs that activate acetylcholine (ACh) receptors, while the US is encoded by biogenic amine (BA) systems. In recent years, we have advanced our understanding on the specific neural BA pathways and receptors involved in olfactory learning and memory. However, little information exists on the contribution of cholinergic receptors to this process. Here we evaluate for the first time the proposition that, as in mammals, muscarinic ACh receptors (mAChRs) contribute to memory formation in Drosophila. Our results show that pharmacological and genetic blockade of mAChRs in MB disrupts olfactory aversive memory in larvae. This effect is not explained by an alteration in the ability of animals to respond to odorants or to execute motor programs. These results show that mAChRs in MB contribute to generating olfactory memories in Drosophila.http://dx.doi.org/10.1155/2015/658918 |
| spellingShingle | Bryon Silva Claudia Molina-Fernández María Beatriz Ugalde Eduardo I. Tognarelli Cristian Angel Jorge M. Campusano Muscarinic ACh Receptors Contribute to Aversive Olfactory Learning in Drosophila Neural Plasticity |
| title | Muscarinic ACh Receptors Contribute to Aversive Olfactory Learning in Drosophila |
| title_full | Muscarinic ACh Receptors Contribute to Aversive Olfactory Learning in Drosophila |
| title_fullStr | Muscarinic ACh Receptors Contribute to Aversive Olfactory Learning in Drosophila |
| title_full_unstemmed | Muscarinic ACh Receptors Contribute to Aversive Olfactory Learning in Drosophila |
| title_short | Muscarinic ACh Receptors Contribute to Aversive Olfactory Learning in Drosophila |
| title_sort | muscarinic ach receptors contribute to aversive olfactory learning in drosophila |
| url | http://dx.doi.org/10.1155/2015/658918 |
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