Identification and verification of diagnostic biomarkers for deep infiltrating endometriosis based on machine learning algorithms
Abstract This study addresses the challenges in the early diagnosis of deep infiltrating endometriosis (DIE) by exploring the potential role of the deubiquitinating enzyme USP14. By analyzing the GSE141549 dataset from the Gene Expression Omnibus (GEO) database, using bioinformatics methods and thre...
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| Format: | Article |
| Language: | English |
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BMC
2024-11-01
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| Series: | Journal of Biological Engineering |
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| Online Access: | https://doi.org/10.1186/s13036-024-00466-9 |
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| author | Shanping Shi Chao Huang Xiaojian Tang Hua Liu Weiwei Feng Chen Chen |
| author_facet | Shanping Shi Chao Huang Xiaojian Tang Hua Liu Weiwei Feng Chen Chen |
| author_sort | Shanping Shi |
| collection | DOAJ |
| description | Abstract This study addresses the challenges in the early diagnosis of deep infiltrating endometriosis (DIE) by exploring the potential role of the deubiquitinating enzyme USP14. By analyzing the GSE141549 dataset from the Gene Expression Omnibus (GEO) database, using bioinformatics methods and three machine learning algorithms (LASSO, Random Forest, and Support Vector Machine), the key feature gene USP14 was identified. The results indicated that USP14 is significantly upregulated in DIE and exhibits good predictive value (AUC = 0.786). Further analysis revealed the important role of USP14 in muscle function, cellular growth factor response, and maintenance of chromosome structure, and its close association with various immune cell functions. Immunohistochemical staining confirmed the high expression of USP14 in DIE tissues. This study provides a new molecular target for the early diagnosis of DIE, which holds significant clinical implications and potential application value. |
| format | Article |
| id | doaj-art-d65e413f734f492dad7f62387439997c |
| institution | DOAJ |
| issn | 1754-1611 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Biological Engineering |
| spelling | doaj-art-d65e413f734f492dad7f62387439997c2025-08-20T02:49:17ZengBMCJournal of Biological Engineering1754-16112024-11-0118111710.1186/s13036-024-00466-9Identification and verification of diagnostic biomarkers for deep infiltrating endometriosis based on machine learning algorithmsShanping Shi0Chao Huang1Xiaojian Tang2Hua Liu3Weiwei Feng4Chen Chen5Department of Obstetrics and Gynecology, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Obstetrics and Gynecology, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Obstetrics and Gynecology, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Obstetrics and Gynecology, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Obstetrics and Gynecology, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Obstetrics and Gynecology, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineAbstract This study addresses the challenges in the early diagnosis of deep infiltrating endometriosis (DIE) by exploring the potential role of the deubiquitinating enzyme USP14. By analyzing the GSE141549 dataset from the Gene Expression Omnibus (GEO) database, using bioinformatics methods and three machine learning algorithms (LASSO, Random Forest, and Support Vector Machine), the key feature gene USP14 was identified. The results indicated that USP14 is significantly upregulated in DIE and exhibits good predictive value (AUC = 0.786). Further analysis revealed the important role of USP14 in muscle function, cellular growth factor response, and maintenance of chromosome structure, and its close association with various immune cell functions. Immunohistochemical staining confirmed the high expression of USP14 in DIE tissues. This study provides a new molecular target for the early diagnosis of DIE, which holds significant clinical implications and potential application value.https://doi.org/10.1186/s13036-024-00466-9Deep infiltrating endometriosis (DIE)USP14Machine learning algorithmsSingle-cell RNA sequencing (scRNA-seq)Immunohistochemical staining |
| spellingShingle | Shanping Shi Chao Huang Xiaojian Tang Hua Liu Weiwei Feng Chen Chen Identification and verification of diagnostic biomarkers for deep infiltrating endometriosis based on machine learning algorithms Journal of Biological Engineering Deep infiltrating endometriosis (DIE) USP14 Machine learning algorithms Single-cell RNA sequencing (scRNA-seq) Immunohistochemical staining |
| title | Identification and verification of diagnostic biomarkers for deep infiltrating endometriosis based on machine learning algorithms |
| title_full | Identification and verification of diagnostic biomarkers for deep infiltrating endometriosis based on machine learning algorithms |
| title_fullStr | Identification and verification of diagnostic biomarkers for deep infiltrating endometriosis based on machine learning algorithms |
| title_full_unstemmed | Identification and verification of diagnostic biomarkers for deep infiltrating endometriosis based on machine learning algorithms |
| title_short | Identification and verification of diagnostic biomarkers for deep infiltrating endometriosis based on machine learning algorithms |
| title_sort | identification and verification of diagnostic biomarkers for deep infiltrating endometriosis based on machine learning algorithms |
| topic | Deep infiltrating endometriosis (DIE) USP14 Machine learning algorithms Single-cell RNA sequencing (scRNA-seq) Immunohistochemical staining |
| url | https://doi.org/10.1186/s13036-024-00466-9 |
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