Advances in optical biosensors as alternative diagnostics for clinical determination of ESKAPE bacteria

The global rise of antimicrobial resistance (AMR), manifesting as multidrug-resistant, extremely drug-resistant, and pandrug-resistant pathogens, is causing morbidities which are alarmingly translating to mortalities. The issue is pertinent to low and middle-income countries, which rely heavily on t...

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Main Authors: Maargavi Singh, Sreelakshmi C․S․, Chiranjay Mukhopadhyay, Sajan D. George, Pooja Nag, Kapil Sadani
Format: Article
Language:English
Published: Elsevier 2025-12-01
Series:Sensors and Actuators Reports
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666053925000839
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author Maargavi Singh
Sreelakshmi C․S․
Chiranjay Mukhopadhyay
Sajan D. George
Pooja Nag
Kapil Sadani
author_facet Maargavi Singh
Sreelakshmi C․S․
Chiranjay Mukhopadhyay
Sajan D. George
Pooja Nag
Kapil Sadani
author_sort Maargavi Singh
collection DOAJ
description The global rise of antimicrobial resistance (AMR), manifesting as multidrug-resistant, extremely drug-resistant, and pandrug-resistant pathogens, is causing morbidities which are alarmingly translating to mortalities. The issue is pertinent to low and middle-income countries, which rely heavily on their primary and secondary healthcare setups with severely constrained infrastructure and diagnostics. Traditional and molecular diagnostic methods are effective, but have long turnaround times, are expensive, and require specialized facilities. Due to these constraints, these facilities are usually not present at the primary healthcare centers. This review explores the urgent need for alternative diagnostic strategies beyond conventional pathogen identification and antibiotic susceptibility testing, emphasizing the detection of bacterial metabolites and virulence factors as innovative biomarkers for AMR. This article provides critical insight into tailoring optical biosensor technologies as alternate diagnostics for ESKAPE pathogens in resource-limited settings. It highlights the integration of these biosensing platforms with emerging metabolomics and biomarker profiling technologies, offering a promising route toward point-of-care diagnostics. In addition, incorporating artificial intelligence and machine learning algorithms in signal processing and feature extraction enhances biosensor performance and accelerates diagnostic accuracy. The review critiques the current state of the art in AMR diagnostics and provides strategic inroads for developing robust and deployable diagnostics to help better bacterial infection control.
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publishDate 2025-12-01
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spelling doaj-art-d65a27df27f74d8a97e87f22079721f62025-08-20T02:52:37ZengElsevierSensors and Actuators Reports2666-05392025-12-011010036510.1016/j.snr.2025.100365Advances in optical biosensors as alternative diagnostics for clinical determination of ESKAPE bacteriaMaargavi Singh0Sreelakshmi C․S․1Chiranjay Mukhopadhyay2Sajan D. George3Pooja Nag4Kapil Sadani5Department of Instrumentation and Control Engineering, Manipal Institute of Technology, Manipal Academy of Higher Education, Manipal, Karnataka, IndiaDepartment of Microbiology, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, Karnataka, IndiaDepartment of Microbiology, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, Karnataka, IndiaCentre for Applied Nanoscience, Manipal Institute of Applied Physics, Manipal Academy of Higher Education, Manipal, Karnataka, IndiaDepartment of Mechatronics, Manipal Institute of Technology, Manipal Academy of Higher Education, Manipal, Karnataka, IndiaDepartment of Instrumentation and Control Engineering, Manipal Institute of Technology, Manipal Academy of Higher Education, Manipal, Karnataka, India; Corresponding author.The global rise of antimicrobial resistance (AMR), manifesting as multidrug-resistant, extremely drug-resistant, and pandrug-resistant pathogens, is causing morbidities which are alarmingly translating to mortalities. The issue is pertinent to low and middle-income countries, which rely heavily on their primary and secondary healthcare setups with severely constrained infrastructure and diagnostics. Traditional and molecular diagnostic methods are effective, but have long turnaround times, are expensive, and require specialized facilities. Due to these constraints, these facilities are usually not present at the primary healthcare centers. This review explores the urgent need for alternative diagnostic strategies beyond conventional pathogen identification and antibiotic susceptibility testing, emphasizing the detection of bacterial metabolites and virulence factors as innovative biomarkers for AMR. This article provides critical insight into tailoring optical biosensor technologies as alternate diagnostics for ESKAPE pathogens in resource-limited settings. It highlights the integration of these biosensing platforms with emerging metabolomics and biomarker profiling technologies, offering a promising route toward point-of-care diagnostics. In addition, incorporating artificial intelligence and machine learning algorithms in signal processing and feature extraction enhances biosensor performance and accelerates diagnostic accuracy. The review critiques the current state of the art in AMR diagnostics and provides strategic inroads for developing robust and deployable diagnostics to help better bacterial infection control.http://www.sciencedirect.com/science/article/pii/S2666053925000839AMRESKAPE pathogensBiosensorsBacterial metabolitesSERS
spellingShingle Maargavi Singh
Sreelakshmi C․S․
Chiranjay Mukhopadhyay
Sajan D. George
Pooja Nag
Kapil Sadani
Advances in optical biosensors as alternative diagnostics for clinical determination of ESKAPE bacteria
Sensors and Actuators Reports
AMR
ESKAPE pathogens
Biosensors
Bacterial metabolites
SERS
title Advances in optical biosensors as alternative diagnostics for clinical determination of ESKAPE bacteria
title_full Advances in optical biosensors as alternative diagnostics for clinical determination of ESKAPE bacteria
title_fullStr Advances in optical biosensors as alternative diagnostics for clinical determination of ESKAPE bacteria
title_full_unstemmed Advances in optical biosensors as alternative diagnostics for clinical determination of ESKAPE bacteria
title_short Advances in optical biosensors as alternative diagnostics for clinical determination of ESKAPE bacteria
title_sort advances in optical biosensors as alternative diagnostics for clinical determination of eskape bacteria
topic AMR
ESKAPE pathogens
Biosensors
Bacterial metabolites
SERS
url http://www.sciencedirect.com/science/article/pii/S2666053925000839
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