Protocol for the visualization of pRps6-positive cells in larval zebrafish brains using whole-mount immunofluorescence and light-sheet microscopy

Protocol for the visualization of pRps6-positive cells in larval zebrafish brains using whole-mount immunofluorescence and light-sheet microscopy

Summary: Due to their small size and transparency, larval zebrafish are a useful model for whole-brain imaging. Here, we present a protocol for the visualization of phosphorylated Rps6, a marker of mechanistic target of rapamycin complex 1 (mTORC1) activity, in the zebrafish brains at 5 days post fe...

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Bibliographic Details
Main Authors: Olga Doszyn, Tomasz Dulski, Justyna Zmorzynska
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:STAR Protocols
Subjects:
developmental biology
microscopy
model organisms
neuroscience
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724007524
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