Identification of the Cytotoxic Transglutaminase from <i>Mycobacterium</i> spp. That Is Involved in RIPK1 Activation

Although the global incidence of tuberculosis has declined in recent years, tuberculosis remains a major global public health challenge. The <i>Mycobacterium tuberculosis</i> complex (MTBC) including <i>M. tuberculosis</i>, <i>M. bovis</i>, <i>M. microti<...

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Main Authors: Xinting Zhang, Yikai Zhang, Xiao Feng, Yueying Wang, Si-Shang Li, Mei-Yi Yan, Yi-Cheng Sun, Qi Jin, Feng Jiang
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Molecules
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Online Access:https://www.mdpi.com/1420-3049/30/10/2251
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author Xinting Zhang
Yikai Zhang
Xiao Feng
Yueying Wang
Si-Shang Li
Mei-Yi Yan
Yi-Cheng Sun
Qi Jin
Feng Jiang
author_facet Xinting Zhang
Yikai Zhang
Xiao Feng
Yueying Wang
Si-Shang Li
Mei-Yi Yan
Yi-Cheng Sun
Qi Jin
Feng Jiang
author_sort Xinting Zhang
collection DOAJ
description Although the global incidence of tuberculosis has declined in recent years, tuberculosis remains a major global public health challenge. The <i>Mycobacterium tuberculosis</i> complex (MTBC) including <i>M. tuberculosis</i>, <i>M. bovis</i>, <i>M. microti</i>, etc., is the deadliest <i>Mycobacterium</i> spp. that needs more attention. Research on <i>M. microti</i> is significant as it is a zoonotic pathogen that can spread between animals and humans. By exploring the function of a transglutaminase in <i>M. microti</i> (MmTG), which is widely distributed in <i>Mycobacterium</i> and other species, a potential cytotoxic effector has been characterized. MmTG inhibits cell proliferation by inducing the phosphorylation of RIPK1 (receptor-interacting serine/threonine-protein kinase 1) and the Cys159 of MmTG is the highly conserved residue related to its cytotoxicity. Understanding MmTG and its homologs can provide more insights into the pathogenic mechanisms of mycobacteria and contribute to the development of more effective therapeutic strategies against mycobacterial infections.
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institution Kabale University
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publishDate 2025-05-01
publisher MDPI AG
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series Molecules
spelling doaj-art-d656e2e6b688409db4f1d4ae08d931f32025-08-20T03:48:02ZengMDPI AGMolecules1420-30492025-05-013010225110.3390/molecules30102251Identification of the Cytotoxic Transglutaminase from <i>Mycobacterium</i> spp. That Is Involved in RIPK1 ActivationXinting Zhang0Yikai Zhang1Xiao Feng2Yueying Wang3Si-Shang Li4Mei-Yi Yan5Yi-Cheng Sun6Qi Jin7Feng Jiang8NHC Key Laboratory of Systems Biology of Pathogens, Key Laboratory of Pathogen Infection Prevention and Control (Ministry of Education), State Key Laboratory of Respiratory Health and Multimorbidity, National Institute of Pathogen Biology and Center for Tuberculosis Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 102629, ChinaNHC Key Laboratory of Systems Biology of Pathogens, Key Laboratory of Pathogen Infection Prevention and Control (Ministry of Education), State Key Laboratory of Respiratory Health and Multimorbidity, National Institute of Pathogen Biology and Center for Tuberculosis Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 102629, ChinaNHC Key Laboratory of Systems Biology of Pathogens, Key Laboratory of Pathogen Infection Prevention and Control (Ministry of Education), State Key Laboratory of Respiratory Health and Multimorbidity, National Institute of Pathogen Biology and Center for Tuberculosis Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 102629, ChinaNHC Key Laboratory of Systems Biology of Pathogens, Key Laboratory of Pathogen Infection Prevention and Control (Ministry of Education), State Key Laboratory of Respiratory Health and Multimorbidity, National Institute of Pathogen Biology and Center for Tuberculosis Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 102629, ChinaNHC Key Laboratory of Systems Biology of Pathogens, Key Laboratory of Pathogen Infection Prevention and Control (Ministry of Education), State Key Laboratory of Respiratory Health and Multimorbidity, National Institute of Pathogen Biology and Center for Tuberculosis Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 102629, ChinaNHC Key Laboratory of Systems Biology of Pathogens, Key Laboratory of Pathogen Infection Prevention and Control (Ministry of Education), State Key Laboratory of Respiratory Health and Multimorbidity, National Institute of Pathogen Biology and Center for Tuberculosis Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 102629, ChinaNHC Key Laboratory of Systems Biology of Pathogens, Key Laboratory of Pathogen Infection Prevention and Control (Ministry of Education), State Key Laboratory of Respiratory Health and Multimorbidity, National Institute of Pathogen Biology and Center for Tuberculosis Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 102629, ChinaNHC Key Laboratory of Systems Biology of Pathogens, Key Laboratory of Pathogen Infection Prevention and Control (Ministry of Education), State Key Laboratory of Respiratory Health and Multimorbidity, National Institute of Pathogen Biology and Center for Tuberculosis Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 102629, ChinaNHC Key Laboratory of Systems Biology of Pathogens, Key Laboratory of Pathogen Infection Prevention and Control (Ministry of Education), State Key Laboratory of Respiratory Health and Multimorbidity, National Institute of Pathogen Biology and Center for Tuberculosis Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 102629, ChinaAlthough the global incidence of tuberculosis has declined in recent years, tuberculosis remains a major global public health challenge. The <i>Mycobacterium tuberculosis</i> complex (MTBC) including <i>M. tuberculosis</i>, <i>M. bovis</i>, <i>M. microti</i>, etc., is the deadliest <i>Mycobacterium</i> spp. that needs more attention. Research on <i>M. microti</i> is significant as it is a zoonotic pathogen that can spread between animals and humans. By exploring the function of a transglutaminase in <i>M. microti</i> (MmTG), which is widely distributed in <i>Mycobacterium</i> and other species, a potential cytotoxic effector has been characterized. MmTG inhibits cell proliferation by inducing the phosphorylation of RIPK1 (receptor-interacting serine/threonine-protein kinase 1) and the Cys159 of MmTG is the highly conserved residue related to its cytotoxicity. Understanding MmTG and its homologs can provide more insights into the pathogenic mechanisms of mycobacteria and contribute to the development of more effective therapeutic strategies against mycobacterial infections.https://www.mdpi.com/1420-3049/30/10/2251<i>Mycobacterium</i> spp.MmTGRIPK1cytotoxicitytransglutaminase
spellingShingle Xinting Zhang
Yikai Zhang
Xiao Feng
Yueying Wang
Si-Shang Li
Mei-Yi Yan
Yi-Cheng Sun
Qi Jin
Feng Jiang
Identification of the Cytotoxic Transglutaminase from <i>Mycobacterium</i> spp. That Is Involved in RIPK1 Activation
Molecules
<i>Mycobacterium</i> spp.
MmTG
RIPK1
cytotoxicity
transglutaminase
title Identification of the Cytotoxic Transglutaminase from <i>Mycobacterium</i> spp. That Is Involved in RIPK1 Activation
title_full Identification of the Cytotoxic Transglutaminase from <i>Mycobacterium</i> spp. That Is Involved in RIPK1 Activation
title_fullStr Identification of the Cytotoxic Transglutaminase from <i>Mycobacterium</i> spp. That Is Involved in RIPK1 Activation
title_full_unstemmed Identification of the Cytotoxic Transglutaminase from <i>Mycobacterium</i> spp. That Is Involved in RIPK1 Activation
title_short Identification of the Cytotoxic Transglutaminase from <i>Mycobacterium</i> spp. That Is Involved in RIPK1 Activation
title_sort identification of the cytotoxic transglutaminase from i mycobacterium i spp that is involved in ripk1 activation
topic <i>Mycobacterium</i> spp.
MmTG
RIPK1
cytotoxicity
transglutaminase
url https://www.mdpi.com/1420-3049/30/10/2251
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