Rice straw degradation by Pseudomonas aeruginosa AMB-CD-1, isolated from fresh cow dung and its impact on rice plants
Purpose Rice straw degradation in the soil is slow because of the high percentage of complex carbohydrates. Hence, farmers prefer to burn it in the agricultural field as a quicker alternative to the open burning of rice straw. Therefore, this study aims to characterize the bacteria from cow dung tha...
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| Format: | Article |
| Language: | English |
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OICC Press
2023-12-01
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| Series: | International Journal of Recycling of Organic Waste in Agriculture |
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| Online Access: | https://ijrowa.isfahan.iau.ir/article_696583_6244caf54814f9f0d82208962e4d26b1.pdf |
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| author | Kishan Raj Anup Singh Tapas Chowdhury S Gupta Ravindra Soni |
| author_facet | Kishan Raj Anup Singh Tapas Chowdhury S Gupta Ravindra Soni |
| author_sort | Kishan Raj |
| collection | DOAJ |
| description | Purpose Rice straw degradation in the soil is slow because of the high percentage of complex carbohydrates. Hence, farmers prefer to burn it in the agricultural field as a quicker alternative to the open burning of rice straw. Therefore, this study aims to characterize the bacteria from cow dung that can degrade rice straw.Method In the present study, four bacterial isolates were isolated from the fresh cow-dung sample. After initial screening, one isolate, i.e. AMB-CD-1, was used to degrade rice straw for 25 days. The degraded straw samples were then analyzed through FTIR and SEM analysis. Furthermore, the degraded sample was used for growing paddy plants.Results Out of four isolates, only the AMB-CD-1 isolate performed the best in all the screening analyses. The results concluded that the AMB-CD-1 culture inoculated straw had a low level of cellulose and hemicellulose, indicating these particles’ degradation. The isolated AMB-CD-1 was identified as Pseudomonas aeruginosa through the Sanger sequencing technique of the 16S rRNA gene. Results also revealed that the highest germination percentage, shoot weight and shoot length were found to be significantly superior in T4 (50% Sand + 50% Compost) whereas the highest chlorophyll content was found in T7 (25% Sand + 25% Soil + 50% Compost), and T1 (100% Sand) was effective in terms of root length.Conclusion Overall, the study concluded that Pseudomonas aeruginosa AMB-CD-1 might be a fast decomposer of paddy straw. |
| format | Article |
| id | doaj-art-d653c509a90c4eef898cafe8c9c8ddc8 |
| institution | Kabale University |
| issn | 2195-3228 2251-7715 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | OICC Press |
| record_format | Article |
| series | International Journal of Recycling of Organic Waste in Agriculture |
| spelling | doaj-art-d653c509a90c4eef898cafe8c9c8ddc82025-02-03T09:52:04ZengOICC PressInternational Journal of Recycling of Organic Waste in Agriculture2195-32282251-77152023-12-0112448750210.30486/ijrowa.2022.1950569.1407696583Rice straw degradation by Pseudomonas aeruginosa AMB-CD-1, isolated from fresh cow dung and its impact on rice plantsKishan Raj0Anup Singh1Tapas Chowdhury2S Gupta3Ravindra Soni4Department of Agricultural Microbiology, College of Agriculture, Indira Gandhi Krishi Vishwavidylaya, Raipur, IndiaDepartment of Agricultural Microbiology, College of Agriculture, Indira Gandhi Krishi Vishwavidylaya, Raipur, IndiaDepartment of Agricultural Microbiology, College of Agriculture, Indira Gandhi Krishi Vishwavidylaya, Raipur, IndiaDepartment of Agricultural Microbiology, College of Agriculture, Indira Gandhi Krishi Vishwavidylaya, Raipur, IndiaDepartment of Agricultural Microbiology, College of Agriculture, Indira Gandhi Krishi Vishwavidylaya, Raipur, IndiaPurpose Rice straw degradation in the soil is slow because of the high percentage of complex carbohydrates. Hence, farmers prefer to burn it in the agricultural field as a quicker alternative to the open burning of rice straw. Therefore, this study aims to characterize the bacteria from cow dung that can degrade rice straw.Method In the present study, four bacterial isolates were isolated from the fresh cow-dung sample. After initial screening, one isolate, i.e. AMB-CD-1, was used to degrade rice straw for 25 days. The degraded straw samples were then analyzed through FTIR and SEM analysis. Furthermore, the degraded sample was used for growing paddy plants.Results Out of four isolates, only the AMB-CD-1 isolate performed the best in all the screening analyses. The results concluded that the AMB-CD-1 culture inoculated straw had a low level of cellulose and hemicellulose, indicating these particles’ degradation. The isolated AMB-CD-1 was identified as Pseudomonas aeruginosa through the Sanger sequencing technique of the 16S rRNA gene. Results also revealed that the highest germination percentage, shoot weight and shoot length were found to be significantly superior in T4 (50% Sand + 50% Compost) whereas the highest chlorophyll content was found in T7 (25% Sand + 25% Soil + 50% Compost), and T1 (100% Sand) was effective in terms of root length.Conclusion Overall, the study concluded that Pseudomonas aeruginosa AMB-CD-1 might be a fast decomposer of paddy straw.https://ijrowa.isfahan.iau.ir/article_696583_6244caf54814f9f0d82208962e4d26b1.pdfpseudomonas aeruginosapaddy strawdegraded strawbiodegradation |
| spellingShingle | Kishan Raj Anup Singh Tapas Chowdhury S Gupta Ravindra Soni Rice straw degradation by Pseudomonas aeruginosa AMB-CD-1, isolated from fresh cow dung and its impact on rice plants International Journal of Recycling of Organic Waste in Agriculture pseudomonas aeruginosa paddy straw degraded straw biodegradation |
| title | Rice straw degradation by Pseudomonas aeruginosa AMB-CD-1, isolated from fresh cow dung and its impact on rice plants |
| title_full | Rice straw degradation by Pseudomonas aeruginosa AMB-CD-1, isolated from fresh cow dung and its impact on rice plants |
| title_fullStr | Rice straw degradation by Pseudomonas aeruginosa AMB-CD-1, isolated from fresh cow dung and its impact on rice plants |
| title_full_unstemmed | Rice straw degradation by Pseudomonas aeruginosa AMB-CD-1, isolated from fresh cow dung and its impact on rice plants |
| title_short | Rice straw degradation by Pseudomonas aeruginosa AMB-CD-1, isolated from fresh cow dung and its impact on rice plants |
| title_sort | rice straw degradation by pseudomonas aeruginosa amb cd 1 isolated from fresh cow dung and its impact on rice plants |
| topic | pseudomonas aeruginosa paddy straw degraded straw biodegradation |
| url | https://ijrowa.isfahan.iau.ir/article_696583_6244caf54814f9f0d82208962e4d26b1.pdf |
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