Characterization of the Compounds Present in <i>Bougainvillea x buttiana</i> (var. Rose) with Healing Activity in a Murine Model
<b>Background/Objective:</b> <i>Bougainvillea x buttiana</i> of the Nyctagenaceae family is widely used in traditional Mexican medicine for treating different diseases. This study was planned to estimate the healing effect of the acetonic extract obtained from <i>Bougai...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-05-01
|
| Series: | Pharmaceuticals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8247/18/5/752 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849711589249253376 |
|---|---|
| author | Luís Martínez-Cuevas Mayra Cedillo-Cortezano Blanca Nury Echeverria Guerrero Rodolfo Abarca-Vargas Vera L. Petricevich |
| author_facet | Luís Martínez-Cuevas Mayra Cedillo-Cortezano Blanca Nury Echeverria Guerrero Rodolfo Abarca-Vargas Vera L. Petricevich |
| author_sort | Luís Martínez-Cuevas |
| collection | DOAJ |
| description | <b>Background/Objective:</b> <i>Bougainvillea x buttiana</i> of the Nyctagenaceae family is widely used in traditional Mexican medicine for treating different diseases. This study was planned to estimate the healing effect of the acetonic extract obtained from <i>Bougainvillea x buttiana</i> (var. Rose). <b>Methods:</b> The bracts with flowers were subjected to extraction using maceration and concentrated in vacuo. Fractionation with a similar profile resulted in 11 fractions, which were determined using TLC. A mouse wound excision model was tested to evaluate the wound healing effect of the topical treatment pre-formulated with fractions of acetonic extract, which were determined using image analysis techniques. Cytokine levels present in the sera of mice treated or not treated with the acetonic extract were determined using the ELISA method. <b>Results:</b> The results obtained showed that the crude acetonic extract of <i>B. x buttiana</i> and/or its fractions in a pre-formulated hydrogel had wound healing capacity. The wound contraction rate and the healing speed in groups of animals treated with the pre-formulated crude extract and/or its fractions were significantly higher compared with the negative control (<i>p</i> < 0.001). Fraction 2 demonstrated more significant healing, reduced the production of cytokines such as IL-6 and TNF-α, and enhanced the levels of IL-10. <b>Conclusion:</b> The present study showed that the fractions obtained from the acetonic extract of <i>B. x buttiana</i> bracts were able to accelerate the wound healing process through anti-inflammatory mechanisms by regulating inflammatory cytokines. The results presented demonstrate that the extracts from <i>B. x buttiana</i> contain compounds that may be responsible for their healing properties. |
| format | Article |
| id | doaj-art-d652ee66e2cb4a8095a667aee4744c58 |
| institution | DOAJ |
| issn | 1424-8247 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj-art-d652ee66e2cb4a8095a667aee4744c582025-08-20T03:14:35ZengMDPI AGPharmaceuticals1424-82472025-05-0118575210.3390/ph18050752Characterization of the Compounds Present in <i>Bougainvillea x buttiana</i> (var. Rose) with Healing Activity in a Murine ModelLuís Martínez-Cuevas0Mayra Cedillo-Cortezano1Blanca Nury Echeverria Guerrero2Rodolfo Abarca-Vargas3Vera L. Petricevich4Facultad de Medicina, Universidad Autónoma del Estado de Morelos (UAEM), Calle Leñeros, Esquina Iztaccíhuatl s/n Col. Volcanes, Cuernavaca C.P. 62350, Morelos, MexicoFacultad de Medicina, Universidad Autónoma del Estado de Morelos (UAEM), Calle Leñeros, Esquina Iztaccíhuatl s/n Col. Volcanes, Cuernavaca C.P. 62350, Morelos, MexicoFacultad de Medicina, Universidad Autónoma del Estado de Morelos (UAEM), Calle Leñeros, Esquina Iztaccíhuatl s/n Col. Volcanes, Cuernavaca C.P. 62350, Morelos, MexicoFacultad de Medicina, Universidad Autónoma del Estado de Morelos (UAEM), Calle Leñeros, Esquina Iztaccíhuatl s/n Col. Volcanes, Cuernavaca C.P. 62350, Morelos, MexicoFacultad de Medicina, Universidad Autónoma del Estado de Morelos (UAEM), Calle Leñeros, Esquina Iztaccíhuatl s/n Col. Volcanes, Cuernavaca C.P. 62350, Morelos, Mexico<b>Background/Objective:</b> <i>Bougainvillea x buttiana</i> of the Nyctagenaceae family is widely used in traditional Mexican medicine for treating different diseases. This study was planned to estimate the healing effect of the acetonic extract obtained from <i>Bougainvillea x buttiana</i> (var. Rose). <b>Methods:</b> The bracts with flowers were subjected to extraction using maceration and concentrated in vacuo. Fractionation with a similar profile resulted in 11 fractions, which were determined using TLC. A mouse wound excision model was tested to evaluate the wound healing effect of the topical treatment pre-formulated with fractions of acetonic extract, which were determined using image analysis techniques. Cytokine levels present in the sera of mice treated or not treated with the acetonic extract were determined using the ELISA method. <b>Results:</b> The results obtained showed that the crude acetonic extract of <i>B. x buttiana</i> and/or its fractions in a pre-formulated hydrogel had wound healing capacity. The wound contraction rate and the healing speed in groups of animals treated with the pre-formulated crude extract and/or its fractions were significantly higher compared with the negative control (<i>p</i> < 0.001). Fraction 2 demonstrated more significant healing, reduced the production of cytokines such as IL-6 and TNF-α, and enhanced the levels of IL-10. <b>Conclusion:</b> The present study showed that the fractions obtained from the acetonic extract of <i>B. x buttiana</i> bracts were able to accelerate the wound healing process through anti-inflammatory mechanisms by regulating inflammatory cytokines. The results presented demonstrate that the extracts from <i>B. x buttiana</i> contain compounds that may be responsible for their healing properties.https://www.mdpi.com/1424-8247/18/5/752wound healingacetonic extractfractionscytokines |
| spellingShingle | Luís Martínez-Cuevas Mayra Cedillo-Cortezano Blanca Nury Echeverria Guerrero Rodolfo Abarca-Vargas Vera L. Petricevich Characterization of the Compounds Present in <i>Bougainvillea x buttiana</i> (var. Rose) with Healing Activity in a Murine Model Pharmaceuticals wound healing acetonic extract fractions cytokines |
| title | Characterization of the Compounds Present in <i>Bougainvillea x buttiana</i> (var. Rose) with Healing Activity in a Murine Model |
| title_full | Characterization of the Compounds Present in <i>Bougainvillea x buttiana</i> (var. Rose) with Healing Activity in a Murine Model |
| title_fullStr | Characterization of the Compounds Present in <i>Bougainvillea x buttiana</i> (var. Rose) with Healing Activity in a Murine Model |
| title_full_unstemmed | Characterization of the Compounds Present in <i>Bougainvillea x buttiana</i> (var. Rose) with Healing Activity in a Murine Model |
| title_short | Characterization of the Compounds Present in <i>Bougainvillea x buttiana</i> (var. Rose) with Healing Activity in a Murine Model |
| title_sort | characterization of the compounds present in i bougainvillea x buttiana i var rose with healing activity in a murine model |
| topic | wound healing acetonic extract fractions cytokines |
| url | https://www.mdpi.com/1424-8247/18/5/752 |
| work_keys_str_mv | AT luismartinezcuevas characterizationofthecompoundspresentinibougainvilleaxbuttianaivarrosewithhealingactivityinamurinemodel AT mayracedillocortezano characterizationofthecompoundspresentinibougainvilleaxbuttianaivarrosewithhealingactivityinamurinemodel AT blancanuryecheverriaguerrero characterizationofthecompoundspresentinibougainvilleaxbuttianaivarrosewithhealingactivityinamurinemodel AT rodolfoabarcavargas characterizationofthecompoundspresentinibougainvilleaxbuttianaivarrosewithhealingactivityinamurinemodel AT veralpetricevich characterizationofthecompoundspresentinibougainvilleaxbuttianaivarrosewithhealingactivityinamurinemodel |