Closed-Loop Control of Extrusion-Based Bioprinting Through Real-Time Computer Vision
Bioprinting is the technology that combines the use of living matter and biomaterials to manufacture biological models, tissues, and structures layer by layer for applications in regenerative medicine, drug testing, and tissue engineering. Among bioprinting techniques, extrusion-based methods are th...
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Publishing House of Wrocław Board of Scientific Technical Societies Federation NOT
2025-06-01
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| Series: | Journal of Machine Engineering |
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| Online Access: | http://jmacheng.not.pl/Closed-Loop-Control-of-Extrusion-Based-Bioprinting-Through-Real-Time-Computer-Vision,205422,0,2.html |
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| author | Javier Arduengo Nicolas Hascoët Francisco Chinesta Jean-Yves Hascoet |
| author_facet | Javier Arduengo Nicolas Hascoët Francisco Chinesta Jean-Yves Hascoet |
| author_sort | Javier Arduengo |
| collection | DOAJ |
| description | Bioprinting is the technology that combines the use of living matter and biomaterials to manufacture biological models, tissues, and structures layer by layer for applications in regenerative medicine, drug testing, and tissue engineering. Among bioprinting techniques, extrusion-based methods are the most widely used because of their relative simplicity, affordability, and ability to handle as wide range of biomaterials, including those with high viscosities. However, achieving consistent print quality remains a challenge, as the rheological properties of bioinks are highly variable and sensitive to environmental factors such as temperature. A critical aspect of print quality is maintaining a consistent and predictable line width, as pre-programmed trajectories and design fidelity rely on this parameter being well controlled. This work introduces a closed-loop control system for Extrusion-Based Bioprinting (EBB), utilizing real-time computer vision. The system employs a camera that is placed to monitor the line width immediately after extrusion, enabling real-time feedback to adjust the feedrate of the extrusion mechanism. This approach ensures consistent line widths across a wide range of materials and conditions, addressing the variability that traditionally hampers EBB. The method was validated using a Pluronic hydrogel, achieving closed-loop control over a wide range of target line widths. These findings demonstrate the potential for automated, robust bioprinting with improved reproducibility and precision, advancing the reliability of this technology for biomedical applications. |
| format | Article |
| id | doaj-art-d6446ffb2f95447898d582726f98ef27 |
| institution | Kabale University |
| issn | 1895-7595 2391-8071 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Publishing House of Wrocław Board of Scientific Technical Societies Federation NOT |
| record_format | Article |
| series | Journal of Machine Engineering |
| spelling | doaj-art-d6446ffb2f95447898d582726f98ef272025-08-20T03:45:04ZengPublishing House of Wrocław Board of Scientific Technical Societies Federation NOTJournal of Machine Engineering1895-75952391-80712025-06-01252284210.36897/jme/205422205422Closed-Loop Control of Extrusion-Based Bioprinting Through Real-Time Computer VisionJavier Arduengo0Nicolas Hascoët1Francisco Chinesta2Jean-Yves Hascoet3Rapid Manufacturing Platform, Nantes Université, École Centrale Nantes, CNRS, GeM, UMR 6183, F-44000, FrancePIMM, Arts et Métiers Institute of Technology, CNRS-UMR 8006, F-75013, FrancePIMM, Arts et Métiers Institute of Technology, CNRS-UMR 8006, F-75013, FranceRapid Manufacturing Platform, Nantes Université, École Centrale Nantes, CNRS, GeM, UMR 6183, F-44000, FranceBioprinting is the technology that combines the use of living matter and biomaterials to manufacture biological models, tissues, and structures layer by layer for applications in regenerative medicine, drug testing, and tissue engineering. Among bioprinting techniques, extrusion-based methods are the most widely used because of their relative simplicity, affordability, and ability to handle as wide range of biomaterials, including those with high viscosities. However, achieving consistent print quality remains a challenge, as the rheological properties of bioinks are highly variable and sensitive to environmental factors such as temperature. A critical aspect of print quality is maintaining a consistent and predictable line width, as pre-programmed trajectories and design fidelity rely on this parameter being well controlled. This work introduces a closed-loop control system for Extrusion-Based Bioprinting (EBB), utilizing real-time computer vision. The system employs a camera that is placed to monitor the line width immediately after extrusion, enabling real-time feedback to adjust the feedrate of the extrusion mechanism. This approach ensures consistent line widths across a wide range of materials and conditions, addressing the variability that traditionally hampers EBB. The method was validated using a Pluronic hydrogel, achieving closed-loop control over a wide range of target line widths. These findings demonstrate the potential for automated, robust bioprinting with improved reproducibility and precision, advancing the reliability of this technology for biomedical applications.http://jmacheng.not.pl/Closed-Loop-Control-of-Extrusion-Based-Bioprinting-Through-Real-Time-Computer-Vision,205422,0,2.htmladditive manufacturingmonitoringextrusion-based bioprintingclosed-loop control |
| spellingShingle | Javier Arduengo Nicolas Hascoët Francisco Chinesta Jean-Yves Hascoet Closed-Loop Control of Extrusion-Based Bioprinting Through Real-Time Computer Vision Journal of Machine Engineering additive manufacturing monitoring extrusion-based bioprinting closed-loop control |
| title | Closed-Loop Control of Extrusion-Based Bioprinting Through Real-Time Computer Vision |
| title_full | Closed-Loop Control of Extrusion-Based Bioprinting Through Real-Time Computer Vision |
| title_fullStr | Closed-Loop Control of Extrusion-Based Bioprinting Through Real-Time Computer Vision |
| title_full_unstemmed | Closed-Loop Control of Extrusion-Based Bioprinting Through Real-Time Computer Vision |
| title_short | Closed-Loop Control of Extrusion-Based Bioprinting Through Real-Time Computer Vision |
| title_sort | closed loop control of extrusion based bioprinting through real time computer vision |
| topic | additive manufacturing monitoring extrusion-based bioprinting closed-loop control |
| url | http://jmacheng.not.pl/Closed-Loop-Control-of-Extrusion-Based-Bioprinting-Through-Real-Time-Computer-Vision,205422,0,2.html |
| work_keys_str_mv | AT javierarduengo closedloopcontrolofextrusionbasedbioprintingthroughrealtimecomputervision AT nicolashascoet closedloopcontrolofextrusionbasedbioprintingthroughrealtimecomputervision AT franciscochinesta closedloopcontrolofextrusionbasedbioprintingthroughrealtimecomputervision AT jeanyveshascoet closedloopcontrolofextrusionbasedbioprintingthroughrealtimecomputervision |