Odontoblasts or odontocytes, expression of stem cells markers and differentiation markers among human adult odontoblasts

Objective: Despite that, the odontoblasts of the dental pulp are considered a terminally differentiated type of cell. We were interested in investigating if they express any embryonic, mesenchymal, or neural stem cell markers, along with other differentiation markers they were reported to express pr...

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Main Authors: Heba Alzer, Firas Alsoleihat
Format: Article
Language:English
Published: Springer 2024-06-01
Series:Saudi Dental Journal
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S1013905224000993
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author Heba Alzer
Firas Alsoleihat
author_facet Heba Alzer
Firas Alsoleihat
author_sort Heba Alzer
collection DOAJ
description Objective: Despite that, the odontoblasts of the dental pulp are considered a terminally differentiated type of cell. We were interested in investigating if they express any embryonic, mesenchymal, or neural stem cell markers, along with other differentiation markers they were reported to express previously. Methods: An immunohistochemistry study was performed on wisdom teeth extracted from healthy donors aged between 17 and 19 for dental reasons. Nine markers were tested: c-Myc, SOX2, MCAM, CD73, NCAM1, STRO1, osteocalcin, S100, and Thy1. Results: Odontoblasts expressed the following markers: embryonic stem cell markers SOX2, c-Myc, mesenchymal stem cell marker MCAM, the neural differentiation marker S100, and the osteogenic differentiation marker osteocalcin. Odontoblasts did not express the following markers: mesenchymal stem cell markers CD73, STRO1, Thy1, and neural stem cell marker NCAM1. Conclusion: These findings suggest that odontoblasts’ expression of these stem cell markers may enable them to dedifferentiate under certain conditions. Further investigation is needed into whether dental materials could induce such dedifferentiation for functional dentin regeneration.
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spelling doaj-art-d63d0ccf9b3b42aa8371ec163a2fc3992025-08-20T02:56:24ZengSpringerSaudi Dental Journal1013-90522024-06-0136689489810.1016/j.sdentj.2024.03.011Odontoblasts or odontocytes, expression of stem cells markers and differentiation markers among human adult odontoblastsHeba Alzer0Firas Alsoleihat1Department of Restorative Dentistry, School of Dentistry, University of Jordan, Amman 11942, Jordan; Corresponding author at: Department of Restorative Dentistry, School of Dentistry, The University of Jordan, Amman 11942 Jordan.Department of Restorative Dentistry, School of Dentistry, University of Jordan, Amman 11942, Jordan; Department of Restorative Dentistry and Basic Medical Sciences, Faculty of Dentistry, University of Petra, Amman 11196, JordanObjective: Despite that, the odontoblasts of the dental pulp are considered a terminally differentiated type of cell. We were interested in investigating if they express any embryonic, mesenchymal, or neural stem cell markers, along with other differentiation markers they were reported to express previously. Methods: An immunohistochemistry study was performed on wisdom teeth extracted from healthy donors aged between 17 and 19 for dental reasons. Nine markers were tested: c-Myc, SOX2, MCAM, CD73, NCAM1, STRO1, osteocalcin, S100, and Thy1. Results: Odontoblasts expressed the following markers: embryonic stem cell markers SOX2, c-Myc, mesenchymal stem cell marker MCAM, the neural differentiation marker S100, and the osteogenic differentiation marker osteocalcin. Odontoblasts did not express the following markers: mesenchymal stem cell markers CD73, STRO1, Thy1, and neural stem cell marker NCAM1. Conclusion: These findings suggest that odontoblasts’ expression of these stem cell markers may enable them to dedifferentiate under certain conditions. Further investigation is needed into whether dental materials could induce such dedifferentiation for functional dentin regeneration.http://www.sciencedirect.com/science/article/pii/S1013905224000993OdontoblastsHuman dental pulp stem cellsEmbryonic stem cells markerNeural stem cells markerMesenchymal stem cells markerNeural crest
spellingShingle Heba Alzer
Firas Alsoleihat
Odontoblasts or odontocytes, expression of stem cells markers and differentiation markers among human adult odontoblasts
Saudi Dental Journal
Odontoblasts
Human dental pulp stem cells
Embryonic stem cells marker
Neural stem cells marker
Mesenchymal stem cells marker
Neural crest
title Odontoblasts or odontocytes, expression of stem cells markers and differentiation markers among human adult odontoblasts
title_full Odontoblasts or odontocytes, expression of stem cells markers and differentiation markers among human adult odontoblasts
title_fullStr Odontoblasts or odontocytes, expression of stem cells markers and differentiation markers among human adult odontoblasts
title_full_unstemmed Odontoblasts or odontocytes, expression of stem cells markers and differentiation markers among human adult odontoblasts
title_short Odontoblasts or odontocytes, expression of stem cells markers and differentiation markers among human adult odontoblasts
title_sort odontoblasts or odontocytes expression of stem cells markers and differentiation markers among human adult odontoblasts
topic Odontoblasts
Human dental pulp stem cells
Embryonic stem cells marker
Neural stem cells marker
Mesenchymal stem cells marker
Neural crest
url http://www.sciencedirect.com/science/article/pii/S1013905224000993
work_keys_str_mv AT hebaalzer odontoblastsorodontocytesexpressionofstemcellsmarkersanddifferentiationmarkersamonghumanadultodontoblasts
AT firasalsoleihat odontoblastsorodontocytesexpressionofstemcellsmarkersanddifferentiationmarkersamonghumanadultodontoblasts