Screening of host proteins interacting with the African swine fever virus outer membrane protein CD2v
BackgroundThe African swine fever virus (ASFV) is a highly pathogenic double-stranded DNA virus that poses a significant threat to the global swine industry. Although the CD2v protein encoded by ASFV is a key factor in viral immune evasion and pathogenicity, the mechanism underlying its interaction...
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Frontiers Media S.A.
2025-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1585335/full |
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| author | Xiong-nan Chen Xiong-nan Chen Xiong-nan Chen Gao-xi Zhu Gao-xi Zhu Gao-xi Zhu Yi-xian Li Yi-han Hao Yi-han Hao Yi-han Hao Yi-fan Liang Yi-fan Liang Chen Hu Ying-shuo Sun Ying-shuo Sun Yun-zhao Peng Yun-zhao Peng Xi Li Xi Li Xi Li Zhao Huang Zhao Huang Zhao Huang Gui-hong Zhang Gui-hong Zhang Gui-hong Zhang Lang Gong Lang Gong Lang Gong Ze-zhong Zheng Ze-zhong Zheng Ze-zhong Zheng Ze-zhong Zheng |
| author_facet | Xiong-nan Chen Xiong-nan Chen Xiong-nan Chen Gao-xi Zhu Gao-xi Zhu Gao-xi Zhu Yi-xian Li Yi-han Hao Yi-han Hao Yi-han Hao Yi-fan Liang Yi-fan Liang Chen Hu Ying-shuo Sun Ying-shuo Sun Yun-zhao Peng Yun-zhao Peng Xi Li Xi Li Xi Li Zhao Huang Zhao Huang Zhao Huang Gui-hong Zhang Gui-hong Zhang Gui-hong Zhang Lang Gong Lang Gong Lang Gong Ze-zhong Zheng Ze-zhong Zheng Ze-zhong Zheng Ze-zhong Zheng |
| author_sort | Xiong-nan Chen |
| collection | DOAJ |
| description | BackgroundThe African swine fever virus (ASFV) is a highly pathogenic double-stranded DNA virus that poses a significant threat to the global swine industry. Although the CD2v protein encoded by ASFV is a key factor in viral immune evasion and pathogenicity, the mechanism underlying its interaction with host proteins remains unclear.MethodsThe aim of this study was to identify a range of potential host proteins that interact with the CD2v protein using the membrane yeast two-hybrid technology.ResultsThrough subsequent validation experiments and functional analyses, we discovered that these proteins are involved in critical cellular processes such as translational regulation, inflammatory responses, immune signaling, and iron metabolism. Furthermore, interaction network and functional enrichment analyses revealed that ASFV might influence host cell functions through multiple pathways to facilitate viral replication.ConclusionThis study provides new insights into the pathogenic mechanisms of ASFV and offers valuable clues for identifying antiviral targets. |
| format | Article |
| id | doaj-art-d63b101e76904a92a13a7dff571b80c2 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-d63b101e76904a92a13a7dff571b80c22025-08-20T03:29:48ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-06-011610.3389/fmicb.2025.15853351585335Screening of host proteins interacting with the African swine fever virus outer membrane protein CD2vXiong-nan Chen0Xiong-nan Chen1Xiong-nan Chen2Gao-xi Zhu3Gao-xi Zhu4Gao-xi Zhu5Yi-xian Li6Yi-han Hao7Yi-han Hao8Yi-han Hao9Yi-fan Liang10Yi-fan Liang11Chen Hu12Ying-shuo Sun13Ying-shuo Sun14Yun-zhao Peng15Yun-zhao Peng16Xi Li17Xi Li18Xi Li19Zhao Huang20Zhao Huang21Zhao Huang22Gui-hong Zhang23Gui-hong Zhang24Gui-hong Zhang25Lang Gong26Lang Gong27Lang Gong28Ze-zhong Zheng29Ze-zhong Zheng30Ze-zhong Zheng31Ze-zhong Zheng32Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaResearch Center for African Swine Fever Prevention and Control, South China Agricultural University, Guangzhou, ChinaMaoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, ChinaGuangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaResearch Center for African Swine Fever Prevention and Control, South China Agricultural University, Guangzhou, ChinaMaoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, ChinaSchool of Medicine, Shenzhen Campus of Sun Yat-sen University, Sun Yat-sen University, Shenzhen, ChinaGuangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaResearch Center for African Swine Fever Prevention and Control, South China Agricultural University, Guangzhou, ChinaMaoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, ChinaGuangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaMaoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, ChinaGuangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaMaoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, ChinaGuangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaMaoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, ChinaGuangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaResearch Center for African Swine Fever Prevention and Control, South China Agricultural University, Guangzhou, ChinaMaoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, ChinaGuangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaResearch Center for African Swine Fever Prevention and Control, South China Agricultural University, Guangzhou, ChinaMaoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, ChinaGuangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaMaoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, ChinaKey Laboratory of Animal Vaccine Development, Ministry of Agriculture and Rural Affairs, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaMaoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, ChinaKey Laboratory of Animal Vaccine Development, Ministry of Agriculture and Rural Affairs, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaResearch Center for African Swine Fever Prevention and Control, South China Agricultural University, Guangzhou, ChinaMaoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, ChinaWen’s Food Group, Yunfu, ChinaBackgroundThe African swine fever virus (ASFV) is a highly pathogenic double-stranded DNA virus that poses a significant threat to the global swine industry. Although the CD2v protein encoded by ASFV is a key factor in viral immune evasion and pathogenicity, the mechanism underlying its interaction with host proteins remains unclear.MethodsThe aim of this study was to identify a range of potential host proteins that interact with the CD2v protein using the membrane yeast two-hybrid technology.ResultsThrough subsequent validation experiments and functional analyses, we discovered that these proteins are involved in critical cellular processes such as translational regulation, inflammatory responses, immune signaling, and iron metabolism. Furthermore, interaction network and functional enrichment analyses revealed that ASFV might influence host cell functions through multiple pathways to facilitate viral replication.ConclusionThis study provides new insights into the pathogenic mechanisms of ASFV and offers valuable clues for identifying antiviral targets.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1585335/fullAfrican swine fever virusCD2vdual membrane systemmembrane protein interactioninteraction network |
| spellingShingle | Xiong-nan Chen Xiong-nan Chen Xiong-nan Chen Gao-xi Zhu Gao-xi Zhu Gao-xi Zhu Yi-xian Li Yi-han Hao Yi-han Hao Yi-han Hao Yi-fan Liang Yi-fan Liang Chen Hu Ying-shuo Sun Ying-shuo Sun Yun-zhao Peng Yun-zhao Peng Xi Li Xi Li Xi Li Zhao Huang Zhao Huang Zhao Huang Gui-hong Zhang Gui-hong Zhang Gui-hong Zhang Lang Gong Lang Gong Lang Gong Ze-zhong Zheng Ze-zhong Zheng Ze-zhong Zheng Ze-zhong Zheng Screening of host proteins interacting with the African swine fever virus outer membrane protein CD2v Frontiers in Microbiology African swine fever virus CD2v dual membrane system membrane protein interaction interaction network |
| title | Screening of host proteins interacting with the African swine fever virus outer membrane protein CD2v |
| title_full | Screening of host proteins interacting with the African swine fever virus outer membrane protein CD2v |
| title_fullStr | Screening of host proteins interacting with the African swine fever virus outer membrane protein CD2v |
| title_full_unstemmed | Screening of host proteins interacting with the African swine fever virus outer membrane protein CD2v |
| title_short | Screening of host proteins interacting with the African swine fever virus outer membrane protein CD2v |
| title_sort | screening of host proteins interacting with the african swine fever virus outer membrane protein cd2v |
| topic | African swine fever virus CD2v dual membrane system membrane protein interaction interaction network |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1585335/full |
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