In vitro comparison of Enterococcus Faecalis survival in dentinal tubules following root canal therapy with AH plus, endoseal MTA, and cold ceramic sealers
Abstract Background Enterococcus faecalis (E. faecalis) is the most common microorganism responsible for post-endodontic infections, which can penetrate deep into dentinal tubules. This study aimed to compare the survival of E. faecalis in dentinal tubules following root canal therapy with AH Plus (...
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BMC
2024-12-01
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| Series: | BMC Oral Health |
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| Online Access: | https://doi.org/10.1186/s12903-024-05192-8 |
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| author | Fatemeh Mokhtari Jalil Modaresi Ali Hasanzade Salmasi Nima Khamisi Hengameh Zandi Katayoun Lesani |
| author_facet | Fatemeh Mokhtari Jalil Modaresi Ali Hasanzade Salmasi Nima Khamisi Hengameh Zandi Katayoun Lesani |
| author_sort | Fatemeh Mokhtari |
| collection | DOAJ |
| description | Abstract Background Enterococcus faecalis (E. faecalis) is the most common microorganism responsible for post-endodontic infections, which can penetrate deep into dentinal tubules. This study aimed to compare the survival of E. faecalis in dentinal tubules following root canal therapy with AH Plus (AHP), Endoseal MTA (ESM), and cold ceramic (CC) sealers. Methods In this in vitro experimental study, 80 single-canal human teeth were decoronated at the cementoenamel junction. The root canals were cleaned and shaped, and auto-clave sterilized after smear layer removal. The roots were then inoculated with E. faecalis, and incubated for 3 weeks. The teeth were randomly assigned to four groups (n = 20) for root canal obturation with AHP, ESM, and CC sealers and 2% gutta-percha with the cold lateral compaction technique. The fourth group served as the control group. After 30 days, dentin chips were collected from the canals, and cultured on blood agar. The colony count was reported and analyzed by the Kruskal-Wallis test (alpha = 0.05). Results The mean colony count was 23.73 ± 13.84 in the AHP, 34.78 ± 18.75 in the ESM, and 28.47 ± 13.73 in the CC group after 30 days. The difference in this regard was not significant among the three experimental groups (P = 0.102). Conclusions Within the limitations of this in vitro study, the results indicated comparable antibacterial activity of AHP, ESM, and CC sealers against E. faecalis in infected dentinal tubules after 30 days. |
| format | Article |
| id | doaj-art-d6317cebc84a4767ab9f55d4169eb883 |
| institution | OA Journals |
| issn | 1472-6831 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Oral Health |
| spelling | doaj-art-d6317cebc84a4767ab9f55d4169eb8832025-08-20T02:31:42ZengBMCBMC Oral Health1472-68312024-12-012411710.1186/s12903-024-05192-8In vitro comparison of Enterococcus Faecalis survival in dentinal tubules following root canal therapy with AH plus, endoseal MTA, and cold ceramic sealersFatemeh Mokhtari0Jalil Modaresi1Ali Hasanzade Salmasi2Nima Khamisi3Hengameh Zandi4Katayoun Lesani5Department of Endodontics, Dental School, Shahid Sadoughi University of Medical SciencesDepartment of Endodontics, Dental School, Shahid Sadoughi University of Medical SciencesDepartment of Endodontics, Dental School, AJA University of Medical SciencesSchool of Dentistry, Islamic Azad University (Khorasgan Branch)Department of Microbiology, School of Medicine, Shahid Sadoughi University of Medical SciencesDepartment of Oral and Maxillofacial Radiology, School of Dentistry, Iran University of Medical SciencesAbstract Background Enterococcus faecalis (E. faecalis) is the most common microorganism responsible for post-endodontic infections, which can penetrate deep into dentinal tubules. This study aimed to compare the survival of E. faecalis in dentinal tubules following root canal therapy with AH Plus (AHP), Endoseal MTA (ESM), and cold ceramic (CC) sealers. Methods In this in vitro experimental study, 80 single-canal human teeth were decoronated at the cementoenamel junction. The root canals were cleaned and shaped, and auto-clave sterilized after smear layer removal. The roots were then inoculated with E. faecalis, and incubated for 3 weeks. The teeth were randomly assigned to four groups (n = 20) for root canal obturation with AHP, ESM, and CC sealers and 2% gutta-percha with the cold lateral compaction technique. The fourth group served as the control group. After 30 days, dentin chips were collected from the canals, and cultured on blood agar. The colony count was reported and analyzed by the Kruskal-Wallis test (alpha = 0.05). Results The mean colony count was 23.73 ± 13.84 in the AHP, 34.78 ± 18.75 in the ESM, and 28.47 ± 13.73 in the CC group after 30 days. The difference in this regard was not significant among the three experimental groups (P = 0.102). Conclusions Within the limitations of this in vitro study, the results indicated comparable antibacterial activity of AHP, ESM, and CC sealers against E. faecalis in infected dentinal tubules after 30 days.https://doi.org/10.1186/s12903-024-05192-8Anti-bacterial agentsEnterococcus faecalisEpoxy Resin-based root canal sealerRoot canal therapy |
| spellingShingle | Fatemeh Mokhtari Jalil Modaresi Ali Hasanzade Salmasi Nima Khamisi Hengameh Zandi Katayoun Lesani In vitro comparison of Enterococcus Faecalis survival in dentinal tubules following root canal therapy with AH plus, endoseal MTA, and cold ceramic sealers BMC Oral Health Anti-bacterial agents Enterococcus faecalis Epoxy Resin-based root canal sealer Root canal therapy |
| title | In vitro comparison of Enterococcus Faecalis survival in dentinal tubules following root canal therapy with AH plus, endoseal MTA, and cold ceramic sealers |
| title_full | In vitro comparison of Enterococcus Faecalis survival in dentinal tubules following root canal therapy with AH plus, endoseal MTA, and cold ceramic sealers |
| title_fullStr | In vitro comparison of Enterococcus Faecalis survival in dentinal tubules following root canal therapy with AH plus, endoseal MTA, and cold ceramic sealers |
| title_full_unstemmed | In vitro comparison of Enterococcus Faecalis survival in dentinal tubules following root canal therapy with AH plus, endoseal MTA, and cold ceramic sealers |
| title_short | In vitro comparison of Enterococcus Faecalis survival in dentinal tubules following root canal therapy with AH plus, endoseal MTA, and cold ceramic sealers |
| title_sort | in vitro comparison of enterococcus faecalis survival in dentinal tubules following root canal therapy with ah plus endoseal mta and cold ceramic sealers |
| topic | Anti-bacterial agents Enterococcus faecalis Epoxy Resin-based root canal sealer Root canal therapy |
| url | https://doi.org/10.1186/s12903-024-05192-8 |
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