Phytochemical and Antibacterial Investigations of the Extracts and Fractions from the Stem Bark of Hymenaea stigonocarpa Mart. ex Hayne and Effect on Ultrastructure of Staphylococcus aureus Induced by Hydroalcoholic Extract
The aim of this study was to investigate the antimicrobial activity of different extracts and fractions obtained from Hymenaea stigonocarpa stem barks. The cyclohexanic, ethyl acetate, ethanol, aqueous, and hydroalcoholic extracts were obtained by maceration. The hydroalcoholic extract was partitio...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2013-01-01
|
| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/862763 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832547535472820224 |
|---|---|
| author | Gustavo Santiago Dimech Luiz Alberto Lira Soares Magda Assunção Ferreira Anne Gabrielle Vasconcelos de Oliveira Maria da Conceição Carvalho Eulália Azevedo Ximenes |
| author_facet | Gustavo Santiago Dimech Luiz Alberto Lira Soares Magda Assunção Ferreira Anne Gabrielle Vasconcelos de Oliveira Maria da Conceição Carvalho Eulália Azevedo Ximenes |
| author_sort | Gustavo Santiago Dimech |
| collection | DOAJ |
| description | The aim of this study was to investigate the antimicrobial activity of different extracts and fractions obtained from Hymenaea stigonocarpa stem barks. The cyclohexanic, ethyl acetate, ethanol, aqueous, and hydroalcoholic extracts were obtained by maceration. The hydroalcoholic extract was partitioned, which resulted in the ethyl acetate and aqueous fractions. All extracts and fractions were subjected to phytochemical screening and evaluation of total phenol and tannin contents. An HPLC-DAD and ultrastructural alterations analysis were performed. Terpenes and coumarins were detected in the cyclohexanic extract. Flavonoids and condensed tannins were present in the other extracts and fractions. The extracts with the highest contents of tannins, ethanol (EE), hydroalcoholic (HE), and aqueous fraction (AF) showed also the highest antimicrobial activity. The MIC values ranged from 64 to 526 µg/mL. The chromatographic fingerprints suggest the presence of astilbin and other flavonoids in EE and HE. Presence of the thick cell wall, undulating outer layer, abnormal septa, and leakage of the cytoplasmic contents and absence of cell wall and cell lyses were the main alterations observed on Staphylococcus aureus ATCC 33591 after treatment with the Hymenaea stigonocarpa hydroalcoholic extract. The presence of phenolic compounds like flavonoids and tannins is possibly the reason for the antimicrobial activity. |
| format | Article |
| id | doaj-art-d62ebd9c9ea64f31b45addad70bce9b6 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-d62ebd9c9ea64f31b45addad70bce9b62025-02-03T06:44:25ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/862763862763Phytochemical and Antibacterial Investigations of the Extracts and Fractions from the Stem Bark of Hymenaea stigonocarpa Mart. ex Hayne and Effect on Ultrastructure of Staphylococcus aureus Induced by Hydroalcoholic ExtractGustavo Santiago Dimech0Luiz Alberto Lira Soares1Magda Assunção Ferreira2Anne Gabrielle Vasconcelos de Oliveira3Maria da Conceição Carvalho4Eulália Azevedo Ximenes5Laboratório de Fisiologia e Bioquímica de Microorganismos, Departamento de Antibióticos, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, 50670-901 Recife, PE, BrazilLaboratório de Farmacognosia, Departamento de Farmácia Universidade Federal de Pernambuco, Centro de Ciências da Saúde, 50670-901 Recife, PE, BrazilLaboratório de Farmacognosia, Departamento de Farmácia Universidade Federal de Pernambuco, Centro de Ciências da Saúde, 50670-901 Recife, PE, BrazilLaboratório de Biologia Celular e Ultraestrutura, Centro de Tecnologias Estratégicas do Nordeste (CETENE), 50670-901 Recife, PE, BrazilLaboratório de Biologia Celular e Ultraestrutura, Centro de Tecnologias Estratégicas do Nordeste (CETENE), 50670-901 Recife, PE, BrazilLaboratório de Fisiologia e Bioquímica de Microorganismos, Departamento de Antibióticos, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, 50670-901 Recife, PE, BrazilThe aim of this study was to investigate the antimicrobial activity of different extracts and fractions obtained from Hymenaea stigonocarpa stem barks. The cyclohexanic, ethyl acetate, ethanol, aqueous, and hydroalcoholic extracts were obtained by maceration. The hydroalcoholic extract was partitioned, which resulted in the ethyl acetate and aqueous fractions. All extracts and fractions were subjected to phytochemical screening and evaluation of total phenol and tannin contents. An HPLC-DAD and ultrastructural alterations analysis were performed. Terpenes and coumarins were detected in the cyclohexanic extract. Flavonoids and condensed tannins were present in the other extracts and fractions. The extracts with the highest contents of tannins, ethanol (EE), hydroalcoholic (HE), and aqueous fraction (AF) showed also the highest antimicrobial activity. The MIC values ranged from 64 to 526 µg/mL. The chromatographic fingerprints suggest the presence of astilbin and other flavonoids in EE and HE. Presence of the thick cell wall, undulating outer layer, abnormal septa, and leakage of the cytoplasmic contents and absence of cell wall and cell lyses were the main alterations observed on Staphylococcus aureus ATCC 33591 after treatment with the Hymenaea stigonocarpa hydroalcoholic extract. The presence of phenolic compounds like flavonoids and tannins is possibly the reason for the antimicrobial activity.http://dx.doi.org/10.1155/2013/862763 |
| spellingShingle | Gustavo Santiago Dimech Luiz Alberto Lira Soares Magda Assunção Ferreira Anne Gabrielle Vasconcelos de Oliveira Maria da Conceição Carvalho Eulália Azevedo Ximenes Phytochemical and Antibacterial Investigations of the Extracts and Fractions from the Stem Bark of Hymenaea stigonocarpa Mart. ex Hayne and Effect on Ultrastructure of Staphylococcus aureus Induced by Hydroalcoholic Extract The Scientific World Journal |
| title | Phytochemical and Antibacterial Investigations of the Extracts and Fractions from the Stem Bark of Hymenaea stigonocarpa Mart. ex Hayne and Effect on Ultrastructure of Staphylococcus aureus Induced by Hydroalcoholic Extract |
| title_full | Phytochemical and Antibacterial Investigations of the Extracts and Fractions from the Stem Bark of Hymenaea stigonocarpa Mart. ex Hayne and Effect on Ultrastructure of Staphylococcus aureus Induced by Hydroalcoholic Extract |
| title_fullStr | Phytochemical and Antibacterial Investigations of the Extracts and Fractions from the Stem Bark of Hymenaea stigonocarpa Mart. ex Hayne and Effect on Ultrastructure of Staphylococcus aureus Induced by Hydroalcoholic Extract |
| title_full_unstemmed | Phytochemical and Antibacterial Investigations of the Extracts and Fractions from the Stem Bark of Hymenaea stigonocarpa Mart. ex Hayne and Effect on Ultrastructure of Staphylococcus aureus Induced by Hydroalcoholic Extract |
| title_short | Phytochemical and Antibacterial Investigations of the Extracts and Fractions from the Stem Bark of Hymenaea stigonocarpa Mart. ex Hayne and Effect on Ultrastructure of Staphylococcus aureus Induced by Hydroalcoholic Extract |
| title_sort | phytochemical and antibacterial investigations of the extracts and fractions from the stem bark of hymenaea stigonocarpa mart ex hayne and effect on ultrastructure of staphylococcus aureus induced by hydroalcoholic extract |
| url | http://dx.doi.org/10.1155/2013/862763 |
| work_keys_str_mv | AT gustavosantiagodimech phytochemicalandantibacterialinvestigationsoftheextractsandfractionsfromthestembarkofhymenaeastigonocarpamartexhayneandeffectonultrastructureofstaphylococcusaureusinducedbyhydroalcoholicextract AT luizalbertolirasoares phytochemicalandantibacterialinvestigationsoftheextractsandfractionsfromthestembarkofhymenaeastigonocarpamartexhayneandeffectonultrastructureofstaphylococcusaureusinducedbyhydroalcoholicextract AT magdaassuncaoferreira phytochemicalandantibacterialinvestigationsoftheextractsandfractionsfromthestembarkofhymenaeastigonocarpamartexhayneandeffectonultrastructureofstaphylococcusaureusinducedbyhydroalcoholicextract AT annegabriellevasconcelosdeoliveira phytochemicalandantibacterialinvestigationsoftheextractsandfractionsfromthestembarkofhymenaeastigonocarpamartexhayneandeffectonultrastructureofstaphylococcusaureusinducedbyhydroalcoholicextract AT mariadaconceicaocarvalho phytochemicalandantibacterialinvestigationsoftheextractsandfractionsfromthestembarkofhymenaeastigonocarpamartexhayneandeffectonultrastructureofstaphylococcusaureusinducedbyhydroalcoholicextract AT eulaliaazevedoximenes phytochemicalandantibacterialinvestigationsoftheextractsandfractionsfromthestembarkofhymenaeastigonocarpamartexhayneandeffectonultrastructureofstaphylococcusaureusinducedbyhydroalcoholicextract |