Molecular Detection of Bartonella spp. in China and St. Kitts
Bartonella are vector-borne hemotropic bacteria that infect a wide variety of hosts, including people. While there are PCR assays that can identify individual or groups of Bartonella, there is no reliable molecular method to simultaneously detect all species while maintaining genus specificity and s...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Canadian Journal of Infectious Diseases and Medical Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2019/3209013 |
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| author | Ke Huang Patrick John Kelly Jilei Zhang Yi Yang Weiguo Liu Anwar Kalalah Chengming Wang |
| author_facet | Ke Huang Patrick John Kelly Jilei Zhang Yi Yang Weiguo Liu Anwar Kalalah Chengming Wang |
| author_sort | Ke Huang |
| collection | DOAJ |
| description | Bartonella are vector-borne hemotropic bacteria that infect a wide variety of hosts, including people. While there are PCR assays that can identify individual or groups of Bartonella, there is no reliable molecular method to simultaneously detect all species while maintaining genus specificity and sensitivity. By comparing highly conserved 16S rRNA sequences of the better-recognized Bartonella spp. on GenBank, we selected primers and probes for a genus-specific pan-Bartonella FRET-qPCR. Then, a gltA-based Bartonella PCR was established by selecting primers for a highly variable region of gltA, of which the sequenced amplicons could identify individual Bartonella spp. The pan-Bartonella FRET-qPCR did not detect negative controls (Brucella spp., Anaplasma spp., Rickettsia spp., Coxiella burnetii, and Wolbachia) but reliably detected as few as two copies of the positive control (Bartonella henselae) per reaction. There was complete agreement between the pan-Bartonella FRET-qPCR and the gltA-based Bartonella PCR in detecting Bartonella in convenience test samples from China and St. Kitts: cats (26%; 81/310), Ctenocephalides felis (20%; 12/60), cattle (24%; 23/98), and donkeys (4%; 1/20). Sequencing of the gltA-based Bartonella PCR products revealed B. henselae (70%; 57/81) and B. clarridgeiae (30%; 24/81) in cats and C. felis (67%; 8/12, and 33%; 4/12, respectively) and B. bovis in cattle (23.5%; 23/98) and donkeys (4.0%; 1/24). The pan-Bartonella FRET-qPCR and gltA-based Bartonella PCR we developed are highly sensitive and specific in detecting recognized Bartonella spp. in a single reaction. The pan-Bartonella FRET-qPCR is convenient requiring no gel electrophoresis and providing copy numbers, while the gltA-based Bartonella PCR reliably differentiates individual Bartonella species. The use of these PCRs should greatly facilitate large-scale surveillance studies and the diagnosis of infections in clinical samples. |
| format | Article |
| id | doaj-art-d627db7e155b49e8afeffac100bba144 |
| institution | Kabale University |
| issn | 1712-9532 1918-1493 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Infectious Diseases and Medical Microbiology |
| spelling | doaj-art-d627db7e155b49e8afeffac100bba1442025-02-03T01:24:40ZengWileyCanadian Journal of Infectious Diseases and Medical Microbiology1712-95321918-14932019-01-01201910.1155/2019/32090133209013Molecular Detection of Bartonella spp. in China and St. KittsKe Huang0Patrick John Kelly1Jilei Zhang2Yi Yang3Weiguo Liu4Anwar Kalalah5Chengming Wang6Yangzhou University College of Veterinary Medicine, Yangzhou, Jiangsu 225009, ChinaRoss University School of Veterinary Medicine, Basseterre, Saint Kitts and NevisDepartment of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USAYangzhou University College of Veterinary Medicine, Yangzhou, Jiangsu 225009, ChinaAnhui Science and Technology University, Bengbu, Anhui, ChinaAuburn University College of Veterinary Medicine, Auburn, AL 36849, USAYangzhou University College of Veterinary Medicine, Yangzhou, Jiangsu 225009, ChinaBartonella are vector-borne hemotropic bacteria that infect a wide variety of hosts, including people. While there are PCR assays that can identify individual or groups of Bartonella, there is no reliable molecular method to simultaneously detect all species while maintaining genus specificity and sensitivity. By comparing highly conserved 16S rRNA sequences of the better-recognized Bartonella spp. on GenBank, we selected primers and probes for a genus-specific pan-Bartonella FRET-qPCR. Then, a gltA-based Bartonella PCR was established by selecting primers for a highly variable region of gltA, of which the sequenced amplicons could identify individual Bartonella spp. The pan-Bartonella FRET-qPCR did not detect negative controls (Brucella spp., Anaplasma spp., Rickettsia spp., Coxiella burnetii, and Wolbachia) but reliably detected as few as two copies of the positive control (Bartonella henselae) per reaction. There was complete agreement between the pan-Bartonella FRET-qPCR and the gltA-based Bartonella PCR in detecting Bartonella in convenience test samples from China and St. Kitts: cats (26%; 81/310), Ctenocephalides felis (20%; 12/60), cattle (24%; 23/98), and donkeys (4%; 1/20). Sequencing of the gltA-based Bartonella PCR products revealed B. henselae (70%; 57/81) and B. clarridgeiae (30%; 24/81) in cats and C. felis (67%; 8/12, and 33%; 4/12, respectively) and B. bovis in cattle (23.5%; 23/98) and donkeys (4.0%; 1/24). The pan-Bartonella FRET-qPCR and gltA-based Bartonella PCR we developed are highly sensitive and specific in detecting recognized Bartonella spp. in a single reaction. The pan-Bartonella FRET-qPCR is convenient requiring no gel electrophoresis and providing copy numbers, while the gltA-based Bartonella PCR reliably differentiates individual Bartonella species. The use of these PCRs should greatly facilitate large-scale surveillance studies and the diagnosis of infections in clinical samples.http://dx.doi.org/10.1155/2019/3209013 |
| spellingShingle | Ke Huang Patrick John Kelly Jilei Zhang Yi Yang Weiguo Liu Anwar Kalalah Chengming Wang Molecular Detection of Bartonella spp. in China and St. Kitts Canadian Journal of Infectious Diseases and Medical Microbiology |
| title | Molecular Detection of Bartonella spp. in China and St. Kitts |
| title_full | Molecular Detection of Bartonella spp. in China and St. Kitts |
| title_fullStr | Molecular Detection of Bartonella spp. in China and St. Kitts |
| title_full_unstemmed | Molecular Detection of Bartonella spp. in China and St. Kitts |
| title_short | Molecular Detection of Bartonella spp. in China and St. Kitts |
| title_sort | molecular detection of bartonella spp in china and st kitts |
| url | http://dx.doi.org/10.1155/2019/3209013 |
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