The Influence of <i>Cynips quercusfolii</i> on the Content of Biofunctional Plant Metabolites in Various Morphological Parts of <i>Quercus robur</i>

English oak (<i>Quercus robur</i>) hosts over 200 species of galls formed by insect larvae, most notably the oak gall wasp (<i>Cynips quercusfolii</i>). These galls result from the abnormal growth of plant tissue in response to oviposition, acting as a shelter and nutrient so...

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Main Authors: Anna Przybylska-Balcerek, Kinga Stuper-Szablewska
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Molecules
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Online Access:https://www.mdpi.com/1420-3049/30/13/2687
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author Anna Przybylska-Balcerek
Kinga Stuper-Szablewska
author_facet Anna Przybylska-Balcerek
Kinga Stuper-Szablewska
author_sort Anna Przybylska-Balcerek
collection DOAJ
description English oak (<i>Quercus robur</i>) hosts over 200 species of galls formed by insect larvae, most notably the oak gall wasp (<i>Cynips quercusfolii</i>). These galls result from the abnormal growth of plant tissue in response to oviposition, acting as a shelter and nutrient source for the larvae. In addition, the galls trigger oxidative stress in the host plant, resulting in the increased production of reactive oxygen species (ROS). This stress response promotes the biosynthesis of antioxidant compounds, including phenolic acids, flavonoids, and tannins. To our knowledge, this is the first study to monitor seasonal changes in phenolic acids, flavonoids, and tannins in relation to <i>C. quercusfolii</i> infestation over a complete vegetation cycle using integrated UPLC profiling and statistical modeling PCA. For the first time, the contents of phenolic acids, flavonoids, and tannins were assessed throughout the vegetation cycle—from flowering to acorn fall. Results showed that galls affect the biochemical profile of the whole plant, suggesting a systemic response to local infection. The results provide new insights into oak defense responses and suggest that gall formation may be associated with systemic metabolic shifts potentially involved in stress mitigation. Furthermore, the study supports the further investigation of oak galls as a valuable source of polyphenols for pharmacological and industrial applications.
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spelling doaj-art-d626ed72de604a1ab57846b3e5329def2025-08-20T02:36:30ZengMDPI AGMolecules1420-30492025-06-013013268710.3390/molecules30132687The Influence of <i>Cynips quercusfolii</i> on the Content of Biofunctional Plant Metabolites in Various Morphological Parts of <i>Quercus robur</i>Anna Przybylska-Balcerek0Kinga Stuper-Szablewska1Department of Chemistry, Poznań University of Life Sciences, 60-637 Poznan, PolandDepartment of Chemistry, Poznań University of Life Sciences, 60-637 Poznan, PolandEnglish oak (<i>Quercus robur</i>) hosts over 200 species of galls formed by insect larvae, most notably the oak gall wasp (<i>Cynips quercusfolii</i>). These galls result from the abnormal growth of plant tissue in response to oviposition, acting as a shelter and nutrient source for the larvae. In addition, the galls trigger oxidative stress in the host plant, resulting in the increased production of reactive oxygen species (ROS). This stress response promotes the biosynthesis of antioxidant compounds, including phenolic acids, flavonoids, and tannins. To our knowledge, this is the first study to monitor seasonal changes in phenolic acids, flavonoids, and tannins in relation to <i>C. quercusfolii</i> infestation over a complete vegetation cycle using integrated UPLC profiling and statistical modeling PCA. For the first time, the contents of phenolic acids, flavonoids, and tannins were assessed throughout the vegetation cycle—from flowering to acorn fall. Results showed that galls affect the biochemical profile of the whole plant, suggesting a systemic response to local infection. The results provide new insights into oak defense responses and suggest that gall formation may be associated with systemic metabolic shifts potentially involved in stress mitigation. Furthermore, the study supports the further investigation of oak galls as a valuable source of polyphenols for pharmacological and industrial applications.https://www.mdpi.com/1420-3049/30/13/2687English oak<i>Quercus robur</i>galls<i>Cynips quercusfolii</i>oxidative stress antioxidant compoundsphenolic acids
spellingShingle Anna Przybylska-Balcerek
Kinga Stuper-Szablewska
The Influence of <i>Cynips quercusfolii</i> on the Content of Biofunctional Plant Metabolites in Various Morphological Parts of <i>Quercus robur</i>
Molecules
English oak
<i>Quercus robur</i>
galls
<i>Cynips quercusfolii</i>
oxidative stress antioxidant compounds
phenolic acids
title The Influence of <i>Cynips quercusfolii</i> on the Content of Biofunctional Plant Metabolites in Various Morphological Parts of <i>Quercus robur</i>
title_full The Influence of <i>Cynips quercusfolii</i> on the Content of Biofunctional Plant Metabolites in Various Morphological Parts of <i>Quercus robur</i>
title_fullStr The Influence of <i>Cynips quercusfolii</i> on the Content of Biofunctional Plant Metabolites in Various Morphological Parts of <i>Quercus robur</i>
title_full_unstemmed The Influence of <i>Cynips quercusfolii</i> on the Content of Biofunctional Plant Metabolites in Various Morphological Parts of <i>Quercus robur</i>
title_short The Influence of <i>Cynips quercusfolii</i> on the Content of Biofunctional Plant Metabolites in Various Morphological Parts of <i>Quercus robur</i>
title_sort influence of i cynips quercusfolii i on the content of biofunctional plant metabolites in various morphological parts of i quercus robur i
topic English oak
<i>Quercus robur</i>
galls
<i>Cynips quercusfolii</i>
oxidative stress antioxidant compounds
phenolic acids
url https://www.mdpi.com/1420-3049/30/13/2687
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