Vacuolar sugar transporter OsERD5 increases rice tillering and yield by modulating intracellular hexose homeostasis
The early responsive to dehydration-like (ERDL or ERD) subfamily, subclade within the monosaccharide transporter (MST) superfamily, is crucial in the regulation of growth and seed yield in Arabidopsis. Here, we identified OsERD5 as an AtERDL6 homologue and explored the function of OsERD5. We found t...
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KeAi Communications Co., Ltd.
2025-06-01
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| Series: | Crop Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214514125000911 |
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| author | Mingjuan Li Zhen Li Hongye Li Qidong Zhu Dong Liu Ben Zhang Youpeng Chen Junyan Chen Huimin Zhang Nader Saad Elsayed Zhenhua Zhang |
| author_facet | Mingjuan Li Zhen Li Hongye Li Qidong Zhu Dong Liu Ben Zhang Youpeng Chen Junyan Chen Huimin Zhang Nader Saad Elsayed Zhenhua Zhang |
| author_sort | Mingjuan Li |
| collection | DOAJ |
| description | The early responsive to dehydration-like (ERDL or ERD) subfamily, subclade within the monosaccharide transporter (MST) superfamily, is crucial in the regulation of growth and seed yield in Arabidopsis. Here, we identified OsERD5 as an AtERDL6 homologue and explored the function of OsERD5. We found that OsERD5 overexpression significantly enhanced the tiller number and grain yield of rice. OsERD5 was widely expressed in aboveground tissues, encoded a tonoplast-localized protein, and exhibited transport activities for fructose, glucose and mannose when expressed in yeast. Expression character assay revealed that OsERD5 mediated hexose efflux across tonoplasts and participated in maintaining the diurnal rhythm-regulated intracellular hexose homeostasis. Additional physiological and molecular evidence showed that OsERD5 overexpression promoted vacuolar glucose efflux, enhanced sucrose synthesis and transport, increased sugar content in the shoot base, and promoted rice tillering by activating the synthesis of cytokinin simultaneously repressing strigolactone and gibberellin signaling. This study elucidates the function of OsERD5 and the mechanism underlying the overexpression of OsERD5 increasing rice tillering and yield. |
| format | Article |
| id | doaj-art-d61d93f9a13247eaa6692d18e0e119ff |
| institution | OA Journals |
| issn | 2214-5141 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Crop Journal |
| spelling | doaj-art-d61d93f9a13247eaa6692d18e0e119ff2025-08-20T02:10:32ZengKeAi Communications Co., Ltd.Crop Journal2214-51412025-06-0113371672610.1016/j.cj.2025.03.010Vacuolar sugar transporter OsERD5 increases rice tillering and yield by modulating intracellular hexose homeostasisMingjuan Li0Zhen Li1Hongye Li2Qidong Zhu3Dong Liu4Ben Zhang5Youpeng Chen6Junyan Chen7Huimin Zhang8Nader Saad Elsayed9Zhenhua Zhang10College of Resources, Hunan Agricultural University, Changsha 410128, Hunan, China; Yuelushan Laboratory, Hongqi Road, Changsha 410128, Hunan, ChinaCollege of Resources, Hunan Agricultural University, Changsha 410128, Hunan, ChinaCollege of Resources, Hunan Agricultural University, Changsha 410128, Hunan, ChinaCollege of Resources, Hunan Agricultural University, Changsha 410128, Hunan, ChinaCollege of Resources, Hunan Agricultural University, Changsha 410128, Hunan, ChinaCollege of Resources, Hunan Agricultural University, Changsha 410128, Hunan, ChinaCollege of Resources, Hunan Agricultural University, Changsha 410128, Hunan, ChinaCollege of Resources, Hunan Agricultural University, Changsha 410128, Hunan, ChinaCollege of Resources, Hunan Agricultural University, Changsha 410128, Hunan, ChinaCollege of Resources, Hunan Agricultural University, Changsha 410128, Hunan, China; Soil and Agricultural Chemistry Department, Faculty of Agriculture (Saba-Basha), Alexandria University, Alexandria, EgyptCollege of Resources, Hunan Agricultural University, Changsha 410128, Hunan, China; Yuelushan Laboratory, Hongqi Road, Changsha 410128, Hunan, China; Corresponding author.The early responsive to dehydration-like (ERDL or ERD) subfamily, subclade within the monosaccharide transporter (MST) superfamily, is crucial in the regulation of growth and seed yield in Arabidopsis. Here, we identified OsERD5 as an AtERDL6 homologue and explored the function of OsERD5. We found that OsERD5 overexpression significantly enhanced the tiller number and grain yield of rice. OsERD5 was widely expressed in aboveground tissues, encoded a tonoplast-localized protein, and exhibited transport activities for fructose, glucose and mannose when expressed in yeast. Expression character assay revealed that OsERD5 mediated hexose efflux across tonoplasts and participated in maintaining the diurnal rhythm-regulated intracellular hexose homeostasis. Additional physiological and molecular evidence showed that OsERD5 overexpression promoted vacuolar glucose efflux, enhanced sucrose synthesis and transport, increased sugar content in the shoot base, and promoted rice tillering by activating the synthesis of cytokinin simultaneously repressing strigolactone and gibberellin signaling. This study elucidates the function of OsERD5 and the mechanism underlying the overexpression of OsERD5 increasing rice tillering and yield.http://www.sciencedirect.com/science/article/pii/S2214514125000911Rice (Oryza sativa L.)Monosaccharide transporterCytoplasm sugar homeostasisSucrose transportTillering |
| spellingShingle | Mingjuan Li Zhen Li Hongye Li Qidong Zhu Dong Liu Ben Zhang Youpeng Chen Junyan Chen Huimin Zhang Nader Saad Elsayed Zhenhua Zhang Vacuolar sugar transporter OsERD5 increases rice tillering and yield by modulating intracellular hexose homeostasis Crop Journal Rice (Oryza sativa L.) Monosaccharide transporter Cytoplasm sugar homeostasis Sucrose transport Tillering |
| title | Vacuolar sugar transporter OsERD5 increases rice tillering and yield by modulating intracellular hexose homeostasis |
| title_full | Vacuolar sugar transporter OsERD5 increases rice tillering and yield by modulating intracellular hexose homeostasis |
| title_fullStr | Vacuolar sugar transporter OsERD5 increases rice tillering and yield by modulating intracellular hexose homeostasis |
| title_full_unstemmed | Vacuolar sugar transporter OsERD5 increases rice tillering and yield by modulating intracellular hexose homeostasis |
| title_short | Vacuolar sugar transporter OsERD5 increases rice tillering and yield by modulating intracellular hexose homeostasis |
| title_sort | vacuolar sugar transporter oserd5 increases rice tillering and yield by modulating intracellular hexose homeostasis |
| topic | Rice (Oryza sativa L.) Monosaccharide transporter Cytoplasm sugar homeostasis Sucrose transport Tillering |
| url | http://www.sciencedirect.com/science/article/pii/S2214514125000911 |
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