Targeted and Untargeted Urinary Metabolomics in Exercise Science: A Systematic Review

PURPOSE Physical activity induces metabolic changes in the body. However, the biological mechanisms underlying these metabolic changes remain unclear. Metabolomics has advanced the field of exercise science, although most studies use invasive procedures for sample collection and have low rates of pa...

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Bibliographic Details
Main Authors: Zi-Xiao Yu, Ji-Suk Chang, Ah-Ram Kim, Ho-Seong Lee
Format: Article
Language:Korean
Published: The Korean Society of Exercise Physiology 2025-05-01
Series:운동과학
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Online Access:http://ksep-es.org/upload/pdf/ksep-2025-00269.pdf
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Summary:PURPOSE Physical activity induces metabolic changes in the body. However, the biological mechanisms underlying these metabolic changes remain unclear. Metabolomics has advanced the field of exercise science, although most studies use invasive procedures for sample collection and have low rates of participant compliance. METHODS A systematic literature search was conducted between March 30 and April 5, 2024, to identify articles published between January 1, 2004 and January 1, 2024, across four academic databases: Web of Science, Google Scholar, PubMed, and Scopus. RESULTS This review analyzed 34 original research articles on urinary metabolomics in exercise science. NMR and mass spectrometry platforms were used in similar proportions of the studies, accounting for 41.2% (14/34) and 35.3% (12/34) of the studies, respectively. Untargeted approaches dominated research, accounting for 85.3% of studies (n=29), whereas targeted methodologies were exclusively used in only 14.7% (n=5). CONCLUSIONS Urinary metabolomics is rapidly emerging as a valuable tool in the field of exercise science. Untargeted approaches have mainly been used owing to their capability to capture exercise-induced metabolic responses comprehensively. Advancements in analytical technologies are expected to accelerate multi-omics integration, enabling precise characterization of physiological adaptations and supporting personalized strategies for health and performance enhancement.
ISSN:1226-1726
2384-0544