Effects of cold atmospheric plasma on oxidative stability, microstructure, and volatile profile of pork meat

Cold atmospheric plasma (CAP) is an innovative non-thermal technology with potential to improve meat safety and preservation. This study evaluated the effects of CAP on oxidative markers, antioxidant activity, fatty acid profile, volatile composition, surface morphology, muscle microstructural chara...

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Main Authors: Y.K. Oliinychenko, S. Saad, E. Zymvrakaki, I. Mourtzinos, B. Tiwari, A. Ch Stratakos
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Journal of Agriculture and Food Research
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666154325002649
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author Y.K. Oliinychenko
S. Saad
E. Zymvrakaki
I. Mourtzinos
B. Tiwari
A. Ch Stratakos
author_facet Y.K. Oliinychenko
S. Saad
E. Zymvrakaki
I. Mourtzinos
B. Tiwari
A. Ch Stratakos
author_sort Y.K. Oliinychenko
collection DOAJ
description Cold atmospheric plasma (CAP) is an innovative non-thermal technology with potential to improve meat safety and preservation. This study evaluated the effects of CAP on oxidative markers, antioxidant activity, fatty acid profile, volatile composition, surface morphology, muscle microstructural characteristics and texture parameters. CAP was applied using piezoelectric direct discharge technology (PDD), with treatment durations of 0, 6, and 9 min. The results showed a significant increase in oxidation markers after 9 min treatment. Peroxide value and carbonyl content increased by ∼36 % and ∼21 %, respectively, whereas aldehyde content increased by ∼23 % after 6 and 9 min. Antioxidant capacity also increased after 9 min treatment, with total phenolic content increasing by 18 %, 2,2-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) activity by 17 %, and 1,1-Diphenyl-2-picrylhydrazyl (DPPH) activity by 17 % following 6 and 9 min treatment. CAP also modified the fatty acid profile, reducing saturated fats by ∼7 % and increasing monounsaturated fats by ∼11 %. Volatile analysis indicated increased levels of aldehydes, alcohols and ketones along with reduced sulphides, following 6 and 9 min of CAP exposure, with more pronounced changes observed at 9 min. Prolonged CAP exposure increased type IIB myofibre diameter and enhanced texture parameters, with hardness, springiness, and chewiness increasing by ∼35 %, ∼17 %, and ∼42 %, respectively. Surface roughness was most affected after 9 min of treatment. These findings show the need to optimise CAP treatment to enhance its benefits in meat processing while mitigating its adverse effects on meat quality.
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spelling doaj-art-d615fa7bb88f44e2a2903f14dfe0e11e2025-08-20T03:25:08ZengElsevierJournal of Agriculture and Food Research2666-15432025-06-012110189310.1016/j.jafr.2025.101893Effects of cold atmospheric plasma on oxidative stability, microstructure, and volatile profile of pork meatY.K. Oliinychenko0S. Saad1E. Zymvrakaki2I. Mourtzinos3B. Tiwari4A. Ch Stratakos5School of Applied Sciences, College for Health, Science and Society, University of the West of England, Coldharbour Ln, Bristol, BS16 1QY, UKSchool of Applied Sciences, College for Health, Science and Society, University of the West of England, Coldharbour Ln, Bristol, BS16 1QY, UKDepartment of Food Science and Technology, School of Agriculture, Aristotle University of Thessaloniki, 57001, Thessaloniki, GreeceDepartment of Food Science and Technology, School of Agriculture, Aristotle University of Thessaloniki, 57001, Thessaloniki, GreeceDepartment of Food Bioscience, Teagasc Food Research Centre, Teagasc, Ashtown, Dublin 15, D15 DY05, IrelandSchool of Applied Sciences, College for Health, Science and Society, University of the West of England, Coldharbour Ln, Bristol, BS16 1QY, UK; Corresponding author.Cold atmospheric plasma (CAP) is an innovative non-thermal technology with potential to improve meat safety and preservation. This study evaluated the effects of CAP on oxidative markers, antioxidant activity, fatty acid profile, volatile composition, surface morphology, muscle microstructural characteristics and texture parameters. CAP was applied using piezoelectric direct discharge technology (PDD), with treatment durations of 0, 6, and 9 min. The results showed a significant increase in oxidation markers after 9 min treatment. Peroxide value and carbonyl content increased by ∼36 % and ∼21 %, respectively, whereas aldehyde content increased by ∼23 % after 6 and 9 min. Antioxidant capacity also increased after 9 min treatment, with total phenolic content increasing by 18 %, 2,2-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) activity by 17 %, and 1,1-Diphenyl-2-picrylhydrazyl (DPPH) activity by 17 % following 6 and 9 min treatment. CAP also modified the fatty acid profile, reducing saturated fats by ∼7 % and increasing monounsaturated fats by ∼11 %. Volatile analysis indicated increased levels of aldehydes, alcohols and ketones along with reduced sulphides, following 6 and 9 min of CAP exposure, with more pronounced changes observed at 9 min. Prolonged CAP exposure increased type IIB myofibre diameter and enhanced texture parameters, with hardness, springiness, and chewiness increasing by ∼35 %, ∼17 %, and ∼42 %, respectively. Surface roughness was most affected after 9 min of treatment. These findings show the need to optimise CAP treatment to enhance its benefits in meat processing while mitigating its adverse effects on meat quality.http://www.sciencedirect.com/science/article/pii/S2666154325002649MicrostructureMeat volatile profileOxidative stabilityNon-thermal treatment
spellingShingle Y.K. Oliinychenko
S. Saad
E. Zymvrakaki
I. Mourtzinos
B. Tiwari
A. Ch Stratakos
Effects of cold atmospheric plasma on oxidative stability, microstructure, and volatile profile of pork meat
Journal of Agriculture and Food Research
Microstructure
Meat volatile profile
Oxidative stability
Non-thermal treatment
title Effects of cold atmospheric plasma on oxidative stability, microstructure, and volatile profile of pork meat
title_full Effects of cold atmospheric plasma on oxidative stability, microstructure, and volatile profile of pork meat
title_fullStr Effects of cold atmospheric plasma on oxidative stability, microstructure, and volatile profile of pork meat
title_full_unstemmed Effects of cold atmospheric plasma on oxidative stability, microstructure, and volatile profile of pork meat
title_short Effects of cold atmospheric plasma on oxidative stability, microstructure, and volatile profile of pork meat
title_sort effects of cold atmospheric plasma on oxidative stability microstructure and volatile profile of pork meat
topic Microstructure
Meat volatile profile
Oxidative stability
Non-thermal treatment
url http://www.sciencedirect.com/science/article/pii/S2666154325002649
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