In situ magnetic-field-assisted bioprinting process using magnetorheological bioink to obtain engineered muscle constructs
Tissue-engineered anisotropic cell constructs are promising candidates for treating volumetric muscle loss (VML). However, achieving successful cell alignment within macroscale 3D cell constructs for skeletal muscle tissue regeneration remains challenging, owing to difficulties in controlling cell a...
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KeAi Communications Co., Ltd.
2025-03-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X24005279 |
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| author | Hanjun Hwangbo SooJung Chae Dongryeol Ryu GeunHyung Kim |
| author_facet | Hanjun Hwangbo SooJung Chae Dongryeol Ryu GeunHyung Kim |
| author_sort | Hanjun Hwangbo |
| collection | DOAJ |
| description | Tissue-engineered anisotropic cell constructs are promising candidates for treating volumetric muscle loss (VML). However, achieving successful cell alignment within macroscale 3D cell constructs for skeletal muscle tissue regeneration remains challenging, owing to difficulties in controlling cell arrangement within a low-viscosity hydrogel. Herein, we propose the concept of a magnetorheological bioink to manipulate the cellular arrangement within a low-viscosity hydrogel. This bioink consisted of gelatin methacrylate (GelMA), iron oxide nanoparticles, and human adipose stem cells (hASCs). The cell arrangement is regulated by the responsiveness of iron oxide nanoparticles to external magnetic fields. A bioprinting process using ring magnets was developed for in situ bioprinting, resulting in well-aligned 3D cell structures and enhanced mechanotransduction effects on hASCs. In vitro analyses revealed upregulation of cellular activities, including myogenic-related gene expression, in hASCs. When implanted into a VML mouse model, the bioconstructs improved muscle functionality and regeneration, validating the effectiveness of the proposed approach. |
| format | Article |
| id | doaj-art-d614352589784b9396832ae5c28c9aa3 |
| institution | Kabale University |
| issn | 2452-199X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj-art-d614352589784b9396832ae5c28c9aa32025-01-26T05:04:26ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2025-03-0145417433In situ magnetic-field-assisted bioprinting process using magnetorheological bioink to obtain engineered muscle constructsHanjun Hwangbo0SooJung Chae1Dongryeol Ryu2GeunHyung Kim3Department of Precision Medicine, Sungkyunkwan University School of Medicine (SKKU-SOM), Suwon, 16419, Republic of Korea; Institute of Quantum Biophysics, Department of Biophysics, Sungkyunkwan University, Suwon, Gyeonggi-do, 16419, Republic of KoreaDepartment of Precision Medicine, Sungkyunkwan University School of Medicine (SKKU-SOM), Suwon, 16419, Republic of KoreaDepartment of Biomedical Science and Engineering, Gwangju Institute of Science and Technology, Gwangju, Republic of KoreaDepartment of Precision Medicine, Sungkyunkwan University School of Medicine (SKKU-SOM), Suwon, 16419, Republic of Korea; Institute of Quantum Biophysics, Department of Biophysics, Sungkyunkwan University, Suwon, Gyeonggi-do, 16419, Republic of Korea; Biomedical Institute for Convergence at SKKU (BICS), Sungkyunkwan University, Suwon, 16419, Republic of Korea; Corresponding author. Department of Precision Medicine, Sungkyunkwan University School of Medicine (SKKU-SOM), Suwon, 16419, Republic of Korea.Tissue-engineered anisotropic cell constructs are promising candidates for treating volumetric muscle loss (VML). However, achieving successful cell alignment within macroscale 3D cell constructs for skeletal muscle tissue regeneration remains challenging, owing to difficulties in controlling cell arrangement within a low-viscosity hydrogel. Herein, we propose the concept of a magnetorheological bioink to manipulate the cellular arrangement within a low-viscosity hydrogel. This bioink consisted of gelatin methacrylate (GelMA), iron oxide nanoparticles, and human adipose stem cells (hASCs). The cell arrangement is regulated by the responsiveness of iron oxide nanoparticles to external magnetic fields. A bioprinting process using ring magnets was developed for in situ bioprinting, resulting in well-aligned 3D cell structures and enhanced mechanotransduction effects on hASCs. In vitro analyses revealed upregulation of cellular activities, including myogenic-related gene expression, in hASCs. When implanted into a VML mouse model, the bioconstructs improved muscle functionality and regeneration, validating the effectiveness of the proposed approach.http://www.sciencedirect.com/science/article/pii/S2452199X24005279Anisotropic tissueMagnetorheological bioinkVML therapeuticsMagnetic fieldMuscle regeneration |
| spellingShingle | Hanjun Hwangbo SooJung Chae Dongryeol Ryu GeunHyung Kim In situ magnetic-field-assisted bioprinting process using magnetorheological bioink to obtain engineered muscle constructs Bioactive Materials Anisotropic tissue Magnetorheological bioink VML therapeutics Magnetic field Muscle regeneration |
| title | In situ magnetic-field-assisted bioprinting process using magnetorheological bioink to obtain engineered muscle constructs |
| title_full | In situ magnetic-field-assisted bioprinting process using magnetorheological bioink to obtain engineered muscle constructs |
| title_fullStr | In situ magnetic-field-assisted bioprinting process using magnetorheological bioink to obtain engineered muscle constructs |
| title_full_unstemmed | In situ magnetic-field-assisted bioprinting process using magnetorheological bioink to obtain engineered muscle constructs |
| title_short | In situ magnetic-field-assisted bioprinting process using magnetorheological bioink to obtain engineered muscle constructs |
| title_sort | in situ magnetic field assisted bioprinting process using magnetorheological bioink to obtain engineered muscle constructs |
| topic | Anisotropic tissue Magnetorheological bioink VML therapeutics Magnetic field Muscle regeneration |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X24005279 |
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