Somatic embryogenesis regulating shoot regeneration efficiency of Date palm (Phoenix dactylifera L.)
The present study was conducted to estimate the specific potential of regenerated date palm by somatic embryogenesis of tissue culture technique. Collectively total of thirty rooted offshoots were cultured on basal medium complemented with composition of 0.0, 0.5, 1.0, 2.0, 3.0 and 4.0 mg / L of BA...
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Faculty of Science, The University of Azad Jammu & Kashmir, Muzaffarabad
2025-03-01
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| Series: | Kashmir Journal of Science |
| Online Access: | https://kjs.org.pk/index.php/kjs/article/view/128 |
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| author | Allah Jurio Khaskheli shahla Baloch |
| author_facet | Allah Jurio Khaskheli shahla Baloch |
| author_sort | Allah Jurio Khaskheli |
| collection | DOAJ |
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The present study was conducted to estimate the specific potential of regenerated date palm by somatic embryogenesis of tissue culture technique. Collectively total of thirty rooted offshoots were cultured on basal medium complemented with composition of 0.0, 0.5, 1.0, 2.0, 3.0 and 4.0 mg / L of BA and Kinetin. The interpretations on the existence time interval, days reserved to start shoot initiation, total shoots number and shoots length were noted. Date palm (Phoenix dactylifera L.) culture redeveloped on MS basal medium without the addition of BAP (6-benzylaminopurine) and kinetin (Kin) exhibited a significantly lower survival rate (72.50% P < 0.05) and no shoot formation was noticed throughout the experimental duration. The highest concentration (4.0 mg/L) resulted in the shortest time for shoot initiation (17.50 days), whereas lower concentrations (0.5 and 1.0 mg/L) exhibited extended durations (23.50 and 24.20 days, respectively). The maximum number of shoots per explant (5.36) was achieved with 4.0 mg/L BA + Kin, while the concentration of 2.0 mg/L produced an optimal number of shoots (3.30). This study highlights the significance of optimizing BA and Kin concentrations to enhance somatic embryogenesis shoot regeneration efficiency in date palm through tissue culture techniques.
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| format | Article |
| id | doaj-art-d603c7ae9dd84692a8d24cef5459afef |
| institution | DOAJ |
| issn | 2958-7832 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Faculty of Science, The University of Azad Jammu & Kashmir, Muzaffarabad |
| record_format | Article |
| series | Kashmir Journal of Science |
| spelling | doaj-art-d603c7ae9dd84692a8d24cef5459afef2025-08-20T02:51:04ZengFaculty of Science, The University of Azad Jammu & Kashmir, MuzaffarabadKashmir Journal of Science2958-78322025-03-0140110.63147/39y1tk23Somatic embryogenesis regulating shoot regeneration efficiency of Date palm (Phoenix dactylifera L.)Allah Jurio Khaskhelishahla Baloch The present study was conducted to estimate the specific potential of regenerated date palm by somatic embryogenesis of tissue culture technique. Collectively total of thirty rooted offshoots were cultured on basal medium complemented with composition of 0.0, 0.5, 1.0, 2.0, 3.0 and 4.0 mg / L of BA and Kinetin. The interpretations on the existence time interval, days reserved to start shoot initiation, total shoots number and shoots length were noted. Date palm (Phoenix dactylifera L.) culture redeveloped on MS basal medium without the addition of BAP (6-benzylaminopurine) and kinetin (Kin) exhibited a significantly lower survival rate (72.50% P < 0.05) and no shoot formation was noticed throughout the experimental duration. The highest concentration (4.0 mg/L) resulted in the shortest time for shoot initiation (17.50 days), whereas lower concentrations (0.5 and 1.0 mg/L) exhibited extended durations (23.50 and 24.20 days, respectively). The maximum number of shoots per explant (5.36) was achieved with 4.0 mg/L BA + Kin, while the concentration of 2.0 mg/L produced an optimal number of shoots (3.30). This study highlights the significance of optimizing BA and Kin concentrations to enhance somatic embryogenesis shoot regeneration efficiency in date palm through tissue culture techniques. https://kjs.org.pk/index.php/kjs/article/view/128 |
| spellingShingle | Allah Jurio Khaskheli shahla Baloch Somatic embryogenesis regulating shoot regeneration efficiency of Date palm (Phoenix dactylifera L.) Kashmir Journal of Science |
| title | Somatic embryogenesis regulating shoot regeneration efficiency of Date palm (Phoenix dactylifera L.) |
| title_full | Somatic embryogenesis regulating shoot regeneration efficiency of Date palm (Phoenix dactylifera L.) |
| title_fullStr | Somatic embryogenesis regulating shoot regeneration efficiency of Date palm (Phoenix dactylifera L.) |
| title_full_unstemmed | Somatic embryogenesis regulating shoot regeneration efficiency of Date palm (Phoenix dactylifera L.) |
| title_short | Somatic embryogenesis regulating shoot regeneration efficiency of Date palm (Phoenix dactylifera L.) |
| title_sort | somatic embryogenesis regulating shoot regeneration efficiency of date palm phoenix dactylifera l |
| url | https://kjs.org.pk/index.php/kjs/article/view/128 |
| work_keys_str_mv | AT allahjuriokhaskheli somaticembryogenesisregulatingshootregenerationefficiencyofdatepalmphoenixdactyliferal AT shahlabaloch somaticembryogenesisregulatingshootregenerationefficiencyofdatepalmphoenixdactyliferal |