First vancomycin-resistant blood isolate of Enterococcus faecium in a children's hospital and molecular analysis of the mechanism of resistance
The first clinical isolate of vancomycin-resistant Enterococcus spp. in Hacettepe University Children's Hospital was isolated from a blood culture of a patient hospitalized in the intensive care unit. He had been on vancomycin therapy for the last four months for consecutive pneumoniae a...
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| Format: | Article |
| Language: | English |
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Hacettepe University Institute of Child Health
2008-12-01
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| Series: | The Turkish Journal of Pediatrics |
| Online Access: | https://turkjpediatr.org/article/view/2489 |
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| _version_ | 1850041699595714560 |
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| author | Belgin Altun A Bülent Cengiz Ateş Kara Mehmet Ceyhan Serhat Unal Gülten Seçmeer Deniz Gür |
| author_facet | Belgin Altun A Bülent Cengiz Ateş Kara Mehmet Ceyhan Serhat Unal Gülten Seçmeer Deniz Gür |
| author_sort | Belgin Altun |
| collection | DOAJ |
| description |
The first clinical isolate of vancomycin-resistant Enterococcus spp. in Hacettepe University Children's Hospital was isolated from a blood culture of a patient hospitalized in the intensive care unit. He had been on vancomycin therapy for the last four months for consecutive pneumoniae and sepsis. The isolate was identified as Enterococcus faecium (E. faecium) and minimal inhibitor concentration (MIC) values were determined as >256 microg/ml and 256 microg/ml for vancomycin and teicoplanin, respectively, with E-test. The isolate was shown to carry the vanA gene with polymerase chain reaction (PCR). Twelve colonizing strains were isolated from the surveillance cultures during the same period and identified as E. faecium, and were also shown to carry the vanA gene. However, arbitrarily-primed-PCR and pulsed-field gel electrophoresis results could not confirm the source of the resistant strain nor did they suggest a clonal spread in the hospital.
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| format | Article |
| id | doaj-art-d5fe7c19acd9458eb11dbe12c591314e |
| institution | DOAJ |
| issn | 0041-4301 2791-6421 |
| language | English |
| publishDate | 2008-12-01 |
| publisher | Hacettepe University Institute of Child Health |
| record_format | Article |
| series | The Turkish Journal of Pediatrics |
| spelling | doaj-art-d5fe7c19acd9458eb11dbe12c591314e2025-08-20T02:55:42ZengHacettepe University Institute of Child HealthThe Turkish Journal of Pediatrics0041-43012791-64212008-12-01506First vancomycin-resistant blood isolate of Enterococcus faecium in a children's hospital and molecular analysis of the mechanism of resistanceBelgin Altun0A Bülent CengizAteş KaraMehmet CeyhanSerhat UnalGülten SeçmeerDeniz GürDepartment of Internal Medicine, Hacettepe University Faculty of Medicine, Ankara, Turkey. The first clinical isolate of vancomycin-resistant Enterococcus spp. in Hacettepe University Children's Hospital was isolated from a blood culture of a patient hospitalized in the intensive care unit. He had been on vancomycin therapy for the last four months for consecutive pneumoniae and sepsis. The isolate was identified as Enterococcus faecium (E. faecium) and minimal inhibitor concentration (MIC) values were determined as >256 microg/ml and 256 microg/ml for vancomycin and teicoplanin, respectively, with E-test. The isolate was shown to carry the vanA gene with polymerase chain reaction (PCR). Twelve colonizing strains were isolated from the surveillance cultures during the same period and identified as E. faecium, and were also shown to carry the vanA gene. However, arbitrarily-primed-PCR and pulsed-field gel electrophoresis results could not confirm the source of the resistant strain nor did they suggest a clonal spread in the hospital. https://turkjpediatr.org/article/view/2489 |
| spellingShingle | Belgin Altun A Bülent Cengiz Ateş Kara Mehmet Ceyhan Serhat Unal Gülten Seçmeer Deniz Gür First vancomycin-resistant blood isolate of Enterococcus faecium in a children's hospital and molecular analysis of the mechanism of resistance The Turkish Journal of Pediatrics |
| title | First vancomycin-resistant blood isolate of Enterococcus faecium in a children's hospital and molecular analysis of the mechanism of resistance |
| title_full | First vancomycin-resistant blood isolate of Enterococcus faecium in a children's hospital and molecular analysis of the mechanism of resistance |
| title_fullStr | First vancomycin-resistant blood isolate of Enterococcus faecium in a children's hospital and molecular analysis of the mechanism of resistance |
| title_full_unstemmed | First vancomycin-resistant blood isolate of Enterococcus faecium in a children's hospital and molecular analysis of the mechanism of resistance |
| title_short | First vancomycin-resistant blood isolate of Enterococcus faecium in a children's hospital and molecular analysis of the mechanism of resistance |
| title_sort | first vancomycin resistant blood isolate of enterococcus faecium in a children s hospital and molecular analysis of the mechanism of resistance |
| url | https://turkjpediatr.org/article/view/2489 |
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