Screening of Active Compounds Against Porcine Epidemic Diarrhea Virus in <i>Hypericum japonicum</i> Thunb. ex Murray Extracts
Porcine epidemic diarrhea (PED) remains a persistent threat to global swine production, necessitating urgent development of targeted interventions. Our previous research established that <i>Hypericum japonicum</i> Thunb. ex Murray (HJT) extract exhibited significant anti-porcine epidemic...
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2025-06-01
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| author | Hongyu Rao Siqi Liu Hao Wu Wenlong Wang Weiyue Wang Weiwei Su Peibo Li |
| author_facet | Hongyu Rao Siqi Liu Hao Wu Wenlong Wang Weiyue Wang Weiwei Su Peibo Li |
| author_sort | Hongyu Rao |
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| description | Porcine epidemic diarrhea (PED) remains a persistent threat to global swine production, necessitating urgent development of targeted interventions. Our previous research established that <i>Hypericum japonicum</i> Thunb. ex Murray (HJT) extract exhibited significant anti-porcine epidemic diarrhea virus (PEDV) activity both in vivo and in vitro. Nevertheless, the principal bioactive constituents mediating this antiviral activity remained uncharacterized. In this study, it was demonstrated that ethanol eluates with 20% (<i>v</i>/<i>v</i>) and 60% (<i>v</i>/<i>v</i>) ethanol exhibited activity against PEDV. Phytochemical characterization revealed 66 distinct compounds, including 36 flavonoids and 13 organic acids identified as possible antiviral constituents. Among these, taxifolin-7-O-rhamnoside and quercetin-7-rhamnoside were identified as the most potent anti-PEDV components. Notably, neither compound exhibited significant antiviral efficacy as monotherapy. However, co-administration produced a reduction in PEDV-G2 titers. This study mechanistically links taxifolin-7-O-rhamnoside and quercetin-7-rhamnoside as core anti-PEDV phytochemicals in HJT extract. These findings support the further development of HJT as a potential therapeutic for PED. |
| format | Article |
| id | doaj-art-d5eee9cdda5d4d2bb2266f05cd166d97 |
| institution | Kabale University |
| issn | 1999-4915 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
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| series | Viruses |
| spelling | doaj-art-d5eee9cdda5d4d2bb2266f05cd166d972025-08-20T03:32:19ZengMDPI AGViruses1999-49152025-06-0117790010.3390/v17070900Screening of Active Compounds Against Porcine Epidemic Diarrhea Virus in <i>Hypericum japonicum</i> Thunb. ex Murray ExtractsHongyu Rao0Siqi Liu1Hao Wu2Wenlong Wang3Weiyue Wang4Weiwei Su5Peibo Li6Guangdong Provincial Key Laboratory of Plant Stress Biology, State Key Laboratory of Biocontrol, Guangdong Engineering and Technology Research Center for Quality and Efficacy Re-Evaluation of Post Marketed TCM, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaGuangdong Provincial Key Laboratory of Plant Stress Biology, State Key Laboratory of Biocontrol, Guangdong Engineering and Technology Research Center for Quality and Efficacy Re-Evaluation of Post Marketed TCM, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaGuangdong Provincial Key Laboratory of Plant Stress Biology, State Key Laboratory of Biocontrol, Guangdong Engineering and Technology Research Center for Quality and Efficacy Re-Evaluation of Post Marketed TCM, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaGuangdong Provincial Key Laboratory of Plant Stress Biology, State Key Laboratory of Biocontrol, Guangdong Engineering and Technology Research Center for Quality and Efficacy Re-Evaluation of Post Marketed TCM, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaGuangdong Provincial Key Laboratory of Plant Stress Biology, State Key Laboratory of Biocontrol, Guangdong Engineering and Technology Research Center for Quality and Efficacy Re-Evaluation of Post Marketed TCM, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaGuangdong Provincial Key Laboratory of Plant Stress Biology, State Key Laboratory of Biocontrol, Guangdong Engineering and Technology Research Center for Quality and Efficacy Re-Evaluation of Post Marketed TCM, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaGuangdong Provincial Key Laboratory of Plant Stress Biology, State Key Laboratory of Biocontrol, Guangdong Engineering and Technology Research Center for Quality and Efficacy Re-Evaluation of Post Marketed TCM, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaPorcine epidemic diarrhea (PED) remains a persistent threat to global swine production, necessitating urgent development of targeted interventions. Our previous research established that <i>Hypericum japonicum</i> Thunb. ex Murray (HJT) extract exhibited significant anti-porcine epidemic diarrhea virus (PEDV) activity both in vivo and in vitro. Nevertheless, the principal bioactive constituents mediating this antiviral activity remained uncharacterized. In this study, it was demonstrated that ethanol eluates with 20% (<i>v</i>/<i>v</i>) and 60% (<i>v</i>/<i>v</i>) ethanol exhibited activity against PEDV. Phytochemical characterization revealed 66 distinct compounds, including 36 flavonoids and 13 organic acids identified as possible antiviral constituents. Among these, taxifolin-7-O-rhamnoside and quercetin-7-rhamnoside were identified as the most potent anti-PEDV components. Notably, neither compound exhibited significant antiviral efficacy as monotherapy. However, co-administration produced a reduction in PEDV-G2 titers. This study mechanistically links taxifolin-7-O-rhamnoside and quercetin-7-rhamnoside as core anti-PEDV phytochemicals in HJT extract. These findings support the further development of HJT as a potential therapeutic for PED.https://www.mdpi.com/1999-4915/17/7/900<i>Hypericum japonicum</i> Thunb. ex Murrayporcine epidemic diarrhea virusantiviral effectactive compound |
| spellingShingle | Hongyu Rao Siqi Liu Hao Wu Wenlong Wang Weiyue Wang Weiwei Su Peibo Li Screening of Active Compounds Against Porcine Epidemic Diarrhea Virus in <i>Hypericum japonicum</i> Thunb. ex Murray Extracts Viruses <i>Hypericum japonicum</i> Thunb. ex Murray porcine epidemic diarrhea virus antiviral effect active compound |
| title | Screening of Active Compounds Against Porcine Epidemic Diarrhea Virus in <i>Hypericum japonicum</i> Thunb. ex Murray Extracts |
| title_full | Screening of Active Compounds Against Porcine Epidemic Diarrhea Virus in <i>Hypericum japonicum</i> Thunb. ex Murray Extracts |
| title_fullStr | Screening of Active Compounds Against Porcine Epidemic Diarrhea Virus in <i>Hypericum japonicum</i> Thunb. ex Murray Extracts |
| title_full_unstemmed | Screening of Active Compounds Against Porcine Epidemic Diarrhea Virus in <i>Hypericum japonicum</i> Thunb. ex Murray Extracts |
| title_short | Screening of Active Compounds Against Porcine Epidemic Diarrhea Virus in <i>Hypericum japonicum</i> Thunb. ex Murray Extracts |
| title_sort | screening of active compounds against porcine epidemic diarrhea virus in i hypericum japonicum i thunb ex murray extracts |
| topic | <i>Hypericum japonicum</i> Thunb. ex Murray porcine epidemic diarrhea virus antiviral effect active compound |
| url | https://www.mdpi.com/1999-4915/17/7/900 |
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