Vibrio parahaemolyticus isolated from live shellfish marketed in Korea: antibiotic and heavy metal resistance
This study examined the antibiotic and heavy metal resistance properties in Vibrio parahaemolyticus isolated from Korea's five most popular shellfish. Thirty-four V. parahaemolyticus isolates were isolated by conducting biochemical tests and PCR. Isolation frequencies were 4 (cockles), 2 (...
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| Format: | Article |
| Language: | English |
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Faculty of Veterinary Medicine, Chiang Mai University
2024-10-01
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| Series: | Veterinary Integrative Sciences |
| Online Access: | https://he02.tci-thaijo.org/index.php/vis/article/view/270925 |
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| author | Masimbule Vidanalage Kasun Sameera Wickramanayake Sana Majeed Prasanga Madhushani Kumarage Gang-Joon Heo |
| author_facet | Masimbule Vidanalage Kasun Sameera Wickramanayake Sana Majeed Prasanga Madhushani Kumarage Gang-Joon Heo |
| author_sort | Masimbule Vidanalage Kasun Sameera Wickramanayake |
| collection | DOAJ |
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This study examined the antibiotic and heavy metal resistance properties in Vibrio parahaemolyticus isolated from Korea's five most popular shellfish. Thirty-four V. parahaemolyticus isolates were isolated by conducting biochemical tests and PCR. Isolation frequencies were 4 (cockles), 2 (scallops), 2 (mussels), 1 (Pacific abalone), and 25 (white-leg shrimp). The disc diffusion method was employed to detect antibiotic resistance. All isolates were resistant to ampicillin. Piperacillin, colistin, and vancomycin resistance were detected in 82%, 68%, and 50% of the isolates, respectively. Thirty-two isolates were multidrug resistant [Multiple Antibiotic Resistance (MAR) index ≥0.2]. β-lactam resistant blaSHV gene was the most prevalent gene detected in 68% of the isolates in PCR assays. In addition, blaCTX, aac(6’)-Ib, and blsTEM were detected in 21%, 44%, and 6% of the isolates, respectively. Phenotypic resistance to Pb, Cd, and Cu was detected in 25, 4, and 3 isolates, respectively in broth dilution test. CzcA gene was the prevalent detected in 16 isolates, followed by CopA and merA genes detected in 11 and 7 isolates, respectively. The findings of this study suggest that live shellfish marketed in Korea are a potential source of antibiotic-resistant V. parahaemolyticus. |
| format | Article |
| id | doaj-art-d5eab564436a446eadd97f264f32e087 |
| institution | OA Journals |
| issn | 2629-9968 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Faculty of Veterinary Medicine, Chiang Mai University |
| record_format | Article |
| series | Veterinary Integrative Sciences |
| spelling | doaj-art-d5eab564436a446eadd97f264f32e0872025-08-20T01:58:48ZengFaculty of Veterinary Medicine, Chiang Mai UniversityVeterinary Integrative Sciences2629-99682024-10-0123310.12982/VIS.2025.072Vibrio parahaemolyticus isolated from live shellfish marketed in Korea: antibiotic and heavy metal resistanceMasimbule Vidanalage Kasun Sameera WickramanayakeSana MajeedPrasanga Madhushani KumarageGang-Joon Heo This study examined the antibiotic and heavy metal resistance properties in Vibrio parahaemolyticus isolated from Korea's five most popular shellfish. Thirty-four V. parahaemolyticus isolates were isolated by conducting biochemical tests and PCR. Isolation frequencies were 4 (cockles), 2 (scallops), 2 (mussels), 1 (Pacific abalone), and 25 (white-leg shrimp). The disc diffusion method was employed to detect antibiotic resistance. All isolates were resistant to ampicillin. Piperacillin, colistin, and vancomycin resistance were detected in 82%, 68%, and 50% of the isolates, respectively. Thirty-two isolates were multidrug resistant [Multiple Antibiotic Resistance (MAR) index ≥0.2]. β-lactam resistant blaSHV gene was the most prevalent gene detected in 68% of the isolates in PCR assays. In addition, blaCTX, aac(6’)-Ib, and blsTEM were detected in 21%, 44%, and 6% of the isolates, respectively. Phenotypic resistance to Pb, Cd, and Cu was detected in 25, 4, and 3 isolates, respectively in broth dilution test. CzcA gene was the prevalent detected in 16 isolates, followed by CopA and merA genes detected in 11 and 7 isolates, respectively. The findings of this study suggest that live shellfish marketed in Korea are a potential source of antibiotic-resistant V. parahaemolyticus.https://he02.tci-thaijo.org/index.php/vis/article/view/270925 |
| spellingShingle | Masimbule Vidanalage Kasun Sameera Wickramanayake Sana Majeed Prasanga Madhushani Kumarage Gang-Joon Heo Vibrio parahaemolyticus isolated from live shellfish marketed in Korea: antibiotic and heavy metal resistance Veterinary Integrative Sciences |
| title | Vibrio parahaemolyticus isolated from live shellfish marketed in Korea: antibiotic and heavy metal resistance |
| title_full | Vibrio parahaemolyticus isolated from live shellfish marketed in Korea: antibiotic and heavy metal resistance |
| title_fullStr | Vibrio parahaemolyticus isolated from live shellfish marketed in Korea: antibiotic and heavy metal resistance |
| title_full_unstemmed | Vibrio parahaemolyticus isolated from live shellfish marketed in Korea: antibiotic and heavy metal resistance |
| title_short | Vibrio parahaemolyticus isolated from live shellfish marketed in Korea: antibiotic and heavy metal resistance |
| title_sort | vibrio parahaemolyticus isolated from live shellfish marketed in korea antibiotic and heavy metal resistance |
| url | https://he02.tci-thaijo.org/index.php/vis/article/view/270925 |
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