The intrasulcular application effect of bisphosphonate hydrogel toward osteoclast activity and relapse movement
Introduction: Orthodontic relapse occurs after orthodontic treatment and shifting of teeth to unfavorable positions. Bisphosphonates’ effects on bone resorption and relapse prevention have been extensively investigated. However, topical administration, which results in local effect, is still a probl...
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Springer
2021-07-01
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| Series: | Saudi Dental Journal |
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| author | Tita Ratya Utari Ika Dewi Ana Pinandi Sri Pudyani Widya Asmara |
| author_facet | Tita Ratya Utari Ika Dewi Ana Pinandi Sri Pudyani Widya Asmara |
| author_sort | Tita Ratya Utari |
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| description | Introduction: Orthodontic relapse occurs after orthodontic treatment and shifting of teeth to unfavorable positions. Bisphosphonates’ effects on bone resorption and relapse prevention have been extensively investigated. However, topical administration, which results in local effect, is still a problem. Objective: This study aimed to investigate the effect of risedronate with gelatin hydrogel as a carrier to prevent relapse movement by inhibiting osteoclast activity. Methods: Lower incisors of 75 guinea pigs were moved distally using an orthodontic appliance until ±3 mm length. Gelatin hydrogel was fabricated to obtain a semisolid controlled release of 250 (Bis-CR250) and 500 mmol/L risedronate (Bis-CR500) and then applied intrasulcularly into the mesial subperiosteal area of 50 guinea pigs (25 in each group) every 3 days; the rest were the control (Bis-CR000). After 14 days of stabilization, the apparatus was removed. The distance decrease between incisors and the osteoclast number with TRAP staining at 0, 3, 7, 14, and 21 days were measured. ANOVA was used to determine the differences among the different time and experimental groups. Results: Both treatments showed significantly less relapse movement compared to the control (p < 0.05) at 14 and 21 days. Bis-CR500 more effectively inhibited the relapse movement than Bis-CR250 on day 21, indicating a dose dependency in the inhibition. Both treatments showed less osteoclast numbers than control (p < 0.05). Conclusion: Controlled release of bisphosphonate risedronate with a topically administered gelatin hydrogel has shown to be effective in decreasing the tooth relapse movement and osteoclast activity. |
| format | Article |
| id | doaj-art-d5e83fd6940646f7a3f7f4dbb9182942 |
| institution | OA Journals |
| issn | 1013-9052 |
| language | English |
| publishDate | 2021-07-01 |
| publisher | Springer |
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| series | Saudi Dental Journal |
| spelling | doaj-art-d5e83fd6940646f7a3f7f4dbb91829422025-08-20T02:13:51ZengSpringerSaudi Dental Journal1013-90522021-07-0133529229810.1016/j.sdentj.2020.03.003The intrasulcular application effect of bisphosphonate hydrogel toward osteoclast activity and relapse movementTita Ratya Utari0Ika Dewi Ana1Pinandi Sri Pudyani2Widya Asmara3School of Dentistry, Faculty of Medicine and Health Sciences, Universitas Muhammadiyah Yogyakarta, Brawijaya St, Geblagan, Tamantirto, Kec. Kasihan, Bantul, Daerah Istimewa, Yogyakarta 55183, Indonesia; Corresponding author at: School of Dentistry, Faculty of Medicine and Health Sciences, Muhammadiyah University of Yogyakarta, Lingkar Selatan St., Bantul, Yogyakarta 55183, Indonesia.Department of Dental Biomedical Sciences, Faculty of Dentistry, Gadjah Mada University, Denta St., Sekip Utara, Yogyakarta 55281, IndonesiaDepartment of Orthodontics, Faculty of Dentistry, Gadjah Mada University, Denta St., Sekip Utara, Yogyakarta 55281, IndonesiaDepartment of Microbiology, Faculty of Veterinary Medicine, Gadjah Mada University, Fauna St., Karangmalang, Yogyakarta 55281, IndonesiaIntroduction: Orthodontic relapse occurs after orthodontic treatment and shifting of teeth to unfavorable positions. Bisphosphonates’ effects on bone resorption and relapse prevention have been extensively investigated. However, topical administration, which results in local effect, is still a problem. Objective: This study aimed to investigate the effect of risedronate with gelatin hydrogel as a carrier to prevent relapse movement by inhibiting osteoclast activity. Methods: Lower incisors of 75 guinea pigs were moved distally using an orthodontic appliance until ±3 mm length. Gelatin hydrogel was fabricated to obtain a semisolid controlled release of 250 (Bis-CR250) and 500 mmol/L risedronate (Bis-CR500) and then applied intrasulcularly into the mesial subperiosteal area of 50 guinea pigs (25 in each group) every 3 days; the rest were the control (Bis-CR000). After 14 days of stabilization, the apparatus was removed. The distance decrease between incisors and the osteoclast number with TRAP staining at 0, 3, 7, 14, and 21 days were measured. ANOVA was used to determine the differences among the different time and experimental groups. Results: Both treatments showed significantly less relapse movement compared to the control (p < 0.05) at 14 and 21 days. Bis-CR500 more effectively inhibited the relapse movement than Bis-CR250 on day 21, indicating a dose dependency in the inhibition. Both treatments showed less osteoclast numbers than control (p < 0.05). Conclusion: Controlled release of bisphosphonate risedronate with a topically administered gelatin hydrogel has shown to be effective in decreasing the tooth relapse movement and osteoclast activity.http://www.sciencedirect.com/science/article/pii/S1013905219309654RisedronateControlled releaseGelatin hydrogelOrthodonticsRelapse movement |
| spellingShingle | Tita Ratya Utari Ika Dewi Ana Pinandi Sri Pudyani Widya Asmara The intrasulcular application effect of bisphosphonate hydrogel toward osteoclast activity and relapse movement Saudi Dental Journal Risedronate Controlled release Gelatin hydrogel Orthodontics Relapse movement |
| title | The intrasulcular application effect of bisphosphonate hydrogel toward osteoclast activity and relapse movement |
| title_full | The intrasulcular application effect of bisphosphonate hydrogel toward osteoclast activity and relapse movement |
| title_fullStr | The intrasulcular application effect of bisphosphonate hydrogel toward osteoclast activity and relapse movement |
| title_full_unstemmed | The intrasulcular application effect of bisphosphonate hydrogel toward osteoclast activity and relapse movement |
| title_short | The intrasulcular application effect of bisphosphonate hydrogel toward osteoclast activity and relapse movement |
| title_sort | intrasulcular application effect of bisphosphonate hydrogel toward osteoclast activity and relapse movement |
| topic | Risedronate Controlled release Gelatin hydrogel Orthodontics Relapse movement |
| url | http://www.sciencedirect.com/science/article/pii/S1013905219309654 |
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