Enrichment of the Glycyrrhizic Acid from Licorice Roots (Glycyrrhiza glabra L.) by Isoelectric Focused Adsorptive Bubble Chromatography
The main aim of this study was to enrich glycyrrhizic acid ammonium salt known as one of the main compounds of licorice roots (Glycyrrhiza glabra L.) by isoelectric focused adsorptive bubble separation technique with different foaming agents. In the experiments, four bubble separation parameters wer...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2016/7201740 |
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| author | Eyyüp Karaoğul Perihan Parlar Harun Parlar M. Hakkı Alma |
| author_facet | Eyyüp Karaoğul Perihan Parlar Harun Parlar M. Hakkı Alma |
| author_sort | Eyyüp Karaoğul |
| collection | DOAJ |
| description | The main aim of this study was to enrich glycyrrhizic acid ammonium salt known as one of the main compounds of licorice roots (Glycyrrhiza glabra L.) by isoelectric focused adsorptive bubble separation technique with different foaming agents. In the experiments, four bubble separation parameters were used with β-lactoglobulin, albumin bovine, and starch (soluble) preferred as foaming agents and without additives. The enrichment of glycyrrhizic acid ammonium salt into the foam was influenced by different additive substances. The results showed that highest enrichment values were obtained from β-lactoglobulin as much as 368.3 times. The lowest enrichment values (5.9 times) were determined for the application without additive. After enrichment, each experiment of glycyrrhizic acid ammonium salt confirmed that these substances could be quantitatively enriched into the collection vessel with isoelectric focused adsorptive bubble separation technique. The transfer of glycyrrhizic acid ammonium salt into the foam from standard solution in the presence of additive was more efficient than aqueous licorice extract. |
| format | Article |
| id | doaj-art-d5e3bbe4722f402791ed3fe24747869a |
| institution | DOAJ |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-d5e3bbe4722f402791ed3fe24747869a2025-08-20T03:23:38ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732016-01-01201610.1155/2016/72017407201740Enrichment of the Glycyrrhizic Acid from Licorice Roots (Glycyrrhiza glabra L.) by Isoelectric Focused Adsorptive Bubble ChromatographyEyyüp Karaoğul0Perihan Parlar1Harun Parlar2M. Hakkı Alma3Department of Forest Industry Engineering, Faculty of Forestry, Kahramanmaraş Sutcu Imam University, Kahramanmaraş, TurkeyFaculty of Health Sciences, Istanbul Esenyurt University, Esenyurt, 34510 Istanbul, TurkeyDepartment of Chemical-Technical Analysis and Chemical Food Technology, Technical University of Munich, Munich, GermanyDepartment of Forest Industry Engineering, Faculty of Forestry, Kahramanmaraş Sutcu Imam University, Kahramanmaraş, TurkeyThe main aim of this study was to enrich glycyrrhizic acid ammonium salt known as one of the main compounds of licorice roots (Glycyrrhiza glabra L.) by isoelectric focused adsorptive bubble separation technique with different foaming agents. In the experiments, four bubble separation parameters were used with β-lactoglobulin, albumin bovine, and starch (soluble) preferred as foaming agents and without additives. The enrichment of glycyrrhizic acid ammonium salt into the foam was influenced by different additive substances. The results showed that highest enrichment values were obtained from β-lactoglobulin as much as 368.3 times. The lowest enrichment values (5.9 times) were determined for the application without additive. After enrichment, each experiment of glycyrrhizic acid ammonium salt confirmed that these substances could be quantitatively enriched into the collection vessel with isoelectric focused adsorptive bubble separation technique. The transfer of glycyrrhizic acid ammonium salt into the foam from standard solution in the presence of additive was more efficient than aqueous licorice extract.http://dx.doi.org/10.1155/2016/7201740 |
| spellingShingle | Eyyüp Karaoğul Perihan Parlar Harun Parlar M. Hakkı Alma Enrichment of the Glycyrrhizic Acid from Licorice Roots (Glycyrrhiza glabra L.) by Isoelectric Focused Adsorptive Bubble Chromatography Journal of Analytical Methods in Chemistry |
| title | Enrichment of the Glycyrrhizic Acid from Licorice Roots (Glycyrrhiza glabra L.) by Isoelectric Focused Adsorptive Bubble Chromatography |
| title_full | Enrichment of the Glycyrrhizic Acid from Licorice Roots (Glycyrrhiza glabra L.) by Isoelectric Focused Adsorptive Bubble Chromatography |
| title_fullStr | Enrichment of the Glycyrrhizic Acid from Licorice Roots (Glycyrrhiza glabra L.) by Isoelectric Focused Adsorptive Bubble Chromatography |
| title_full_unstemmed | Enrichment of the Glycyrrhizic Acid from Licorice Roots (Glycyrrhiza glabra L.) by Isoelectric Focused Adsorptive Bubble Chromatography |
| title_short | Enrichment of the Glycyrrhizic Acid from Licorice Roots (Glycyrrhiza glabra L.) by Isoelectric Focused Adsorptive Bubble Chromatography |
| title_sort | enrichment of the glycyrrhizic acid from licorice roots glycyrrhiza glabra l by isoelectric focused adsorptive bubble chromatography |
| url | http://dx.doi.org/10.1155/2016/7201740 |
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