Portable UV-C device to treat high flow of infectious aerosols generated during clinical respiratory care
Abstract Respiratory interventions including noninvasive ventilation, continuous positive airway pressure and high-flow nasal oxygen generated infectious aerosols may increase risk of airborne disease (SARS-CoV-2, influenza virus) transmission to healthcare workers. We developed and tested a prototy...
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Nature Portfolio
2024-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-82901-x |
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| author | Richard Vincent David Rapoport Priti Balchandani Joseph Borrello Michael Schotsaert Robert Karlicek Gabriel Laghlali Prajakta Warang Seokchan Park Gagandeep Singh Isabella Morgan James Paredes Raveen Rathnasinghe Jacob Wolf Adolfo García-Sastre |
| author_facet | Richard Vincent David Rapoport Priti Balchandani Joseph Borrello Michael Schotsaert Robert Karlicek Gabriel Laghlali Prajakta Warang Seokchan Park Gagandeep Singh Isabella Morgan James Paredes Raveen Rathnasinghe Jacob Wolf Adolfo García-Sastre |
| author_sort | Richard Vincent |
| collection | DOAJ |
| description | Abstract Respiratory interventions including noninvasive ventilation, continuous positive airway pressure and high-flow nasal oxygen generated infectious aerosols may increase risk of airborne disease (SARS-CoV-2, influenza virus) transmission to healthcare workers. We developed and tested a prototype portable UV-C254 device to sterilize high flows of viral-contaminated air from a simulated patient source at airflow rates of up to 100 l/m. Our device consisted of a central quartz tube surrounded 6 high-output UV-C254 lamps, within a larger cylinder allowing recirculation past the UV-C254 lamps a second time before exiting the device. Testing was with nebulized A/PR/8/34 (H1N1) influenza virus. RNA extraction and qRT-PCR showed virus transited through the prototype. Turning on varying numbers of lamps controlled the dose of UVC. Viability experiments at low, medium and high (100 l/min) flows of contaminated gas were conducted with 6, 4, 2 and 1 lamp activated (single-pass and recirculation were tested). Our data show 5-log reduction in plaque forming units from a single lamp (single- pass and recirculated conditions) at high and low flows. UVC dose at 100 l/m was calculated at 11.6 mJ/cm2 single pass and 104 mJ/cm2 recirculated. The protype device shows high efficacy in killing nebulized influenza virus in a high flow of contaminated air. |
| format | Article |
| id | doaj-art-d5cf3d3ec1714ef88911ccb43f6ae564 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-d5cf3d3ec1714ef88911ccb43f6ae5642025-01-05T12:27:25ZengNature PortfolioScientific Reports2045-23222024-12-011411910.1038/s41598-024-82901-xPortable UV-C device to treat high flow of infectious aerosols generated during clinical respiratory careRichard Vincent0David Rapoport1Priti Balchandani2Joseph Borrello3Michael Schotsaert4Robert Karlicek5Gabriel Laghlali6Prajakta Warang7Seokchan Park8Gagandeep Singh9Isabella Morgan10James ParedesRaveen Rathnasinghe11Jacob Wolf12Adolfo García-Sastre13Division of General Internal Medicine, Department of Medicine, Icahn School of Medicine at Mount SinaiDivision of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, Icahn School of Medicine at Mount SinaiBioMedical Engineering and Imaging Institute, Icahn School of Medicine at Mount SinaiMount Sinai BioDesign, Department of Neurosurgery, Icahn School of Medicine at Mount SinaiDepartment of Microbiology, Icahn School of Medicine at Mount SinaiRensselaer Polytechnic InstituteDepartment of Microbiology, Icahn School of Medicine at Mount SinaiDepartment of Microbiology, Icahn School of Medicine at Mount SinaiDepartment of Microbiology, Icahn School of Medicine at Mount SinaiDepartment of Microbiology, Icahn School of Medicine at Mount SinaiMount Sinai BioDesign, Department of Neurosurgery, Icahn School of Medicine at Mount SinaiDepartment of Microbiology, Icahn School of Medicine at Mount SinaiMount Sinai BioDesign, Department of Neurosurgery, Icahn School of Medicine at Mount SinaiDepartment of Microbiology, Icahn School of Medicine at Mount SinaiAbstract Respiratory interventions including noninvasive ventilation, continuous positive airway pressure and high-flow nasal oxygen generated infectious aerosols may increase risk of airborne disease (SARS-CoV-2, influenza virus) transmission to healthcare workers. We developed and tested a prototype portable UV-C254 device to sterilize high flows of viral-contaminated air from a simulated patient source at airflow rates of up to 100 l/m. Our device consisted of a central quartz tube surrounded 6 high-output UV-C254 lamps, within a larger cylinder allowing recirculation past the UV-C254 lamps a second time before exiting the device. Testing was with nebulized A/PR/8/34 (H1N1) influenza virus. RNA extraction and qRT-PCR showed virus transited through the prototype. Turning on varying numbers of lamps controlled the dose of UVC. Viability experiments at low, medium and high (100 l/min) flows of contaminated gas were conducted with 6, 4, 2 and 1 lamp activated (single-pass and recirculation were tested). Our data show 5-log reduction in plaque forming units from a single lamp (single- pass and recirculated conditions) at high and low flows. UVC dose at 100 l/m was calculated at 11.6 mJ/cm2 single pass and 104 mJ/cm2 recirculated. The protype device shows high efficacy in killing nebulized influenza virus in a high flow of contaminated air.https://doi.org/10.1038/s41598-024-82901-x |
| spellingShingle | Richard Vincent David Rapoport Priti Balchandani Joseph Borrello Michael Schotsaert Robert Karlicek Gabriel Laghlali Prajakta Warang Seokchan Park Gagandeep Singh Isabella Morgan James Paredes Raveen Rathnasinghe Jacob Wolf Adolfo García-Sastre Portable UV-C device to treat high flow of infectious aerosols generated during clinical respiratory care Scientific Reports |
| title | Portable UV-C device to treat high flow of infectious aerosols generated during clinical respiratory care |
| title_full | Portable UV-C device to treat high flow of infectious aerosols generated during clinical respiratory care |
| title_fullStr | Portable UV-C device to treat high flow of infectious aerosols generated during clinical respiratory care |
| title_full_unstemmed | Portable UV-C device to treat high flow of infectious aerosols generated during clinical respiratory care |
| title_short | Portable UV-C device to treat high flow of infectious aerosols generated during clinical respiratory care |
| title_sort | portable uv c device to treat high flow of infectious aerosols generated during clinical respiratory care |
| url | https://doi.org/10.1038/s41598-024-82901-x |
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