Effects of Negative Pressure Wound Therapy on Levels of Angiopoetin-2 and Other Selected Circulating Signaling Molecules in Patients with Diabetic Foot Ulcer
Background and Aims. Diabetic foot ulcers (DFUs) are linked to amputations and premature deaths. Negative pressure wound therapy (NPWT) has been used for DFUs. The mechanism of NPWT’s action may be associated with its influence on circulating molecules. We assessed NPWT’s effect on the plasma levels...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2019/1756798 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832553428642955264 |
|---|---|
| author | Jerzy Hohendorff Anna Drozdz Sebastian Borys Agnieszka H. Ludwig-Slomczynska Beata Kiec-Wilk Ewa L. Stepien Maciej T. Malecki |
| author_facet | Jerzy Hohendorff Anna Drozdz Sebastian Borys Agnieszka H. Ludwig-Slomczynska Beata Kiec-Wilk Ewa L. Stepien Maciej T. Malecki |
| author_sort | Jerzy Hohendorff |
| collection | DOAJ |
| description | Background and Aims. Diabetic foot ulcers (DFUs) are linked to amputations and premature deaths. Negative pressure wound therapy (NPWT) has been used for DFUs. The mechanism of NPWT’s action may be associated with its influence on circulating molecules. We assessed NPWT’s effect on the plasma levels of angiopoietin-2 (Ang2), a key regulator of angiogenesis, and its microvesicular receptors (Tie2) as well as the microvesicles (MVs) themselves in DFU patients. Materials and Methods. We included 69 patients with type 2 diabetes mellitus (T2DM) and neuropathic, noninfected DFUs—49 were treated with NPWT and 20 were treated with standard therapy (ST). Assigning patients to the NPWT group was not random but based on DFU characteristics, especially wound area. Ang2 was measured by ELISA in the entire group, while in a subgroup of 19 individuals on NPWT and 10 on ST, flow cytometry was used to measure Tie2+ and the corresponding isotype control (Iso+) and annexin V (AnnV+) as well as total MVs. Measurements were performed at the beginning and after 8±1 days of therapy. Results. Treatment groups were similar for basic characteristics but differed by their median DFU areas (10.3 (4.2-18.9) vs. 1.3 (0.9-3.4) cm2, p=0.0001). At day 0, no difference was observed in Ang2 levels, total MVs, MV Tie+, and MV AnnV+ between the groups. Ang2 decreased after 8 days in the NPWT group, unlike in the ST group (3.54 (2.40-5.40) vs. 3.32 (2.33-4.61), p=0.02, and 3.19±1.11 vs. 3.19±1.29 ng/mL, p=0.98, respectively). No other parameters were identified that may have been influenced by the NPWT treatment. Conclusion. NPWT in T2DM patients with neuropathic, noninfected DFU seems to lead to reduction of the Ang2 level. Influencing the level of Ang2 may constitute one of NPWT-related mechanisms to accelerate wound healing. |
| format | Article |
| id | doaj-art-d5cecc79f80e45f69b5cd3f150dac8b9 |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-d5cecc79f80e45f69b5cd3f150dac8b92025-02-03T05:54:00ZengWileyJournal of Diabetes Research2314-67452314-67532019-01-01201910.1155/2019/17567981756798Effects of Negative Pressure Wound Therapy on Levels of Angiopoetin-2 and Other Selected Circulating Signaling Molecules in Patients with Diabetic Foot UlcerJerzy Hohendorff0Anna Drozdz1Sebastian Borys2Agnieszka H. Ludwig-Slomczynska3Beata Kiec-Wilk4Ewa L. Stepien5Maciej T. Malecki6Department of Metabolic Diseases, Jagiellonian University Medical College, Krakow, PolandDepartment of Medical Physics, Marian Smoluchowski Institute of Physics, Jagiellonian University, Krakow, PolandDepartment of Metabolic Diseases, Jagiellonian University Medical College, Krakow, PolandCenter for Medical Genomics OMICRON, Jagiellonian University Medical College, Krakow, PolandDepartment of Metabolic Diseases, Jagiellonian University Medical College, Krakow, PolandDepartment of Medical Physics, Marian Smoluchowski Institute of Physics, Jagiellonian University, Krakow, PolandDepartment of Metabolic Diseases, Jagiellonian University Medical College, Krakow, PolandBackground and Aims. Diabetic foot ulcers (DFUs) are linked to amputations and premature deaths. Negative pressure wound therapy (NPWT) has been used for DFUs. The mechanism of NPWT’s action may be associated with its influence on circulating molecules. We assessed NPWT’s effect on the plasma levels of angiopoietin-2 (Ang2), a key regulator of angiogenesis, and its microvesicular receptors (Tie2) as well as the microvesicles (MVs) themselves in DFU patients. Materials and Methods. We included 69 patients with type 2 diabetes mellitus (T2DM) and neuropathic, noninfected DFUs—49 were treated with NPWT and 20 were treated with standard therapy (ST). Assigning patients to the NPWT group was not random but based on DFU characteristics, especially wound area. Ang2 was measured by ELISA in the entire group, while in a subgroup of 19 individuals on NPWT and 10 on ST, flow cytometry was used to measure Tie2+ and the corresponding isotype control (Iso+) and annexin V (AnnV+) as well as total MVs. Measurements were performed at the beginning and after 8±1 days of therapy. Results. Treatment groups were similar for basic characteristics but differed by their median DFU areas (10.3 (4.2-18.9) vs. 1.3 (0.9-3.4) cm2, p=0.0001). At day 0, no difference was observed in Ang2 levels, total MVs, MV Tie+, and MV AnnV+ between the groups. Ang2 decreased after 8 days in the NPWT group, unlike in the ST group (3.54 (2.40-5.40) vs. 3.32 (2.33-4.61), p=0.02, and 3.19±1.11 vs. 3.19±1.29 ng/mL, p=0.98, respectively). No other parameters were identified that may have been influenced by the NPWT treatment. Conclusion. NPWT in T2DM patients with neuropathic, noninfected DFU seems to lead to reduction of the Ang2 level. Influencing the level of Ang2 may constitute one of NPWT-related mechanisms to accelerate wound healing.http://dx.doi.org/10.1155/2019/1756798 |
| spellingShingle | Jerzy Hohendorff Anna Drozdz Sebastian Borys Agnieszka H. Ludwig-Slomczynska Beata Kiec-Wilk Ewa L. Stepien Maciej T. Malecki Effects of Negative Pressure Wound Therapy on Levels of Angiopoetin-2 and Other Selected Circulating Signaling Molecules in Patients with Diabetic Foot Ulcer Journal of Diabetes Research |
| title | Effects of Negative Pressure Wound Therapy on Levels of Angiopoetin-2 and Other Selected Circulating Signaling Molecules in Patients with Diabetic Foot Ulcer |
| title_full | Effects of Negative Pressure Wound Therapy on Levels of Angiopoetin-2 and Other Selected Circulating Signaling Molecules in Patients with Diabetic Foot Ulcer |
| title_fullStr | Effects of Negative Pressure Wound Therapy on Levels of Angiopoetin-2 and Other Selected Circulating Signaling Molecules in Patients with Diabetic Foot Ulcer |
| title_full_unstemmed | Effects of Negative Pressure Wound Therapy on Levels of Angiopoetin-2 and Other Selected Circulating Signaling Molecules in Patients with Diabetic Foot Ulcer |
| title_short | Effects of Negative Pressure Wound Therapy on Levels of Angiopoetin-2 and Other Selected Circulating Signaling Molecules in Patients with Diabetic Foot Ulcer |
| title_sort | effects of negative pressure wound therapy on levels of angiopoetin 2 and other selected circulating signaling molecules in patients with diabetic foot ulcer |
| url | http://dx.doi.org/10.1155/2019/1756798 |
| work_keys_str_mv | AT jerzyhohendorff effectsofnegativepressurewoundtherapyonlevelsofangiopoetin2andotherselectedcirculatingsignalingmoleculesinpatientswithdiabeticfootulcer AT annadrozdz effectsofnegativepressurewoundtherapyonlevelsofangiopoetin2andotherselectedcirculatingsignalingmoleculesinpatientswithdiabeticfootulcer AT sebastianborys effectsofnegativepressurewoundtherapyonlevelsofangiopoetin2andotherselectedcirculatingsignalingmoleculesinpatientswithdiabeticfootulcer AT agnieszkahludwigslomczynska effectsofnegativepressurewoundtherapyonlevelsofangiopoetin2andotherselectedcirculatingsignalingmoleculesinpatientswithdiabeticfootulcer AT beatakiecwilk effectsofnegativepressurewoundtherapyonlevelsofangiopoetin2andotherselectedcirculatingsignalingmoleculesinpatientswithdiabeticfootulcer AT ewalstepien effectsofnegativepressurewoundtherapyonlevelsofangiopoetin2andotherselectedcirculatingsignalingmoleculesinpatientswithdiabeticfootulcer AT maciejtmalecki effectsofnegativepressurewoundtherapyonlevelsofangiopoetin2andotherselectedcirculatingsignalingmoleculesinpatientswithdiabeticfootulcer |