Ameliorative effect of zinc oxide nanoparticles against cutaneous amyloidosis in rats: Histopathology and biochemical study
Primary Localized Cutaneous Amyloidosis (PLCA) is a relatively rare chronic condition considered by amyloid deposition in the dermis without accompanying deposits in internal organs. This study was designed to determine the role of zinc oxide nanoparticles in reducing the development of experimental...
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University of Mosul, College of Veterinary Medicine
2025-07-01
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| Series: | Iraqi Journal of Veterinary Sciences |
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| Online Access: | https://www.vetmedmosul.com/article_188173_1c629ad690b2fc5e86d211e3ee6ce8fb.pdf |
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| author | Anfal A. Salaheldeen Enas S. Mustafa |
| author_facet | Anfal A. Salaheldeen Enas S. Mustafa |
| author_sort | Anfal A. Salaheldeen |
| collection | DOAJ |
| description | Primary Localized Cutaneous Amyloidosis (PLCA) is a relatively rare chronic condition considered by amyloid deposition in the dermis without accompanying deposits in internal organs. This study was designed to determine the role of zinc oxide nanoparticles in reducing the development of experimentally induced amyloidosis lesions in male rats. Forty male rats aged two months were used in this research, randomly divided into four groups, with 10 animals in each group. The Control group (group I) and (Group II) Rat intraperitoneally (IP) inoculated with a dose of ZnO-NPs 5 mg/kg (Group III): Rat IP inoculated with 3.0 ml of sodium caseinate solution (8%) (Group IV): Rat IP injected sodium caseinate with nano zinc oxide (ZnO-NPs). The injection protocol was four days of injection and one day of rest for 60 days. The results showed a significant increase in the level of serum amyloid A and Creative protein in Group III when compared with the group of rats, Group IV, but it did not reach its normal levels when compared with Group I. The results of the gross examination showed the presence of pathological lesions such as alopecia, skin thickening, and redness in Group III, while the pathological changes were less severe in Group IV. Histopathological examination of the skin showed infiltration of mononuclear inflammatory cells in the dermis and subcutaneous layers and around the sweat glands, with congestion of the blood vessels and the presence of edema between the muscle fibers, Zenker's necrosis, atrophy of myocytes, and the deposition of a homogeneous protein substance between the collagen fibers and the muscle fibers and around the blood vessels in G3 after 25 days of experiment lesions were more severe after 60 days of treatment. Histological sections of the skin showed a clear improvement, as represented by the slight infiltration of inflammatory cells with little protein deposition in group IV. The results of the histochemistry examination of the skin samples of the different groups showed a positive reaction to amyloid, with varying intensity depending on the treatment period and the type of substance. This study showed that zinc oxide plays a role in reducing the advance of amyloidosis lesions. |
| format | Article |
| id | doaj-art-d5cde232f523442b9e6c6148adb6b819 |
| institution | DOAJ |
| issn | 1607-3894 2071-1255 |
| language | Arabic |
| publishDate | 2025-07-01 |
| publisher | University of Mosul, College of Veterinary Medicine |
| record_format | Article |
| series | Iraqi Journal of Veterinary Sciences |
| spelling | doaj-art-d5cde232f523442b9e6c6148adb6b8192025-08-20T02:39:40ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552025-07-0139356357310.33899/ijvs.2025.158534.4195188173Ameliorative effect of zinc oxide nanoparticles against cutaneous amyloidosis in rats: Histopathology and biochemical studyAnfal A. Salaheldeen0Enas S. Mustafa1Veterinarian, Private sector, Mosul, IraqDepartment of Pathology and Poultry Diseases, College of Veterinary Medicine, University of Mosul, Mosul, IraqPrimary Localized Cutaneous Amyloidosis (PLCA) is a relatively rare chronic condition considered by amyloid deposition in the dermis without accompanying deposits in internal organs. This study was designed to determine the role of zinc oxide nanoparticles in reducing the development of experimentally induced amyloidosis lesions in male rats. Forty male rats aged two months were used in this research, randomly divided into four groups, with 10 animals in each group. The Control group (group I) and (Group II) Rat intraperitoneally (IP) inoculated with a dose of ZnO-NPs 5 mg/kg (Group III): Rat IP inoculated with 3.0 ml of sodium caseinate solution (8%) (Group IV): Rat IP injected sodium caseinate with nano zinc oxide (ZnO-NPs). The injection protocol was four days of injection and one day of rest for 60 days. The results showed a significant increase in the level of serum amyloid A and Creative protein in Group III when compared with the group of rats, Group IV, but it did not reach its normal levels when compared with Group I. The results of the gross examination showed the presence of pathological lesions such as alopecia, skin thickening, and redness in Group III, while the pathological changes were less severe in Group IV. Histopathological examination of the skin showed infiltration of mononuclear inflammatory cells in the dermis and subcutaneous layers and around the sweat glands, with congestion of the blood vessels and the presence of edema between the muscle fibers, Zenker's necrosis, atrophy of myocytes, and the deposition of a homogeneous protein substance between the collagen fibers and the muscle fibers and around the blood vessels in G3 after 25 days of experiment lesions were more severe after 60 days of treatment. Histological sections of the skin showed a clear improvement, as represented by the slight infiltration of inflammatory cells with little protein deposition in group IV. The results of the histochemistry examination of the skin samples of the different groups showed a positive reaction to amyloid, with varying intensity depending on the treatment period and the type of substance. This study showed that zinc oxide plays a role in reducing the advance of amyloidosis lesions.https://www.vetmedmosul.com/article_188173_1c629ad690b2fc5e86d211e3ee6ce8fb.pdfamyloidosiszinc oxide nanoparticlesaa |
| spellingShingle | Anfal A. Salaheldeen Enas S. Mustafa Ameliorative effect of zinc oxide nanoparticles against cutaneous amyloidosis in rats: Histopathology and biochemical study Iraqi Journal of Veterinary Sciences amyloidosis zinc oxide nanoparticle saa |
| title | Ameliorative effect of zinc oxide nanoparticles against cutaneous amyloidosis in rats: Histopathology and biochemical study |
| title_full | Ameliorative effect of zinc oxide nanoparticles against cutaneous amyloidosis in rats: Histopathology and biochemical study |
| title_fullStr | Ameliorative effect of zinc oxide nanoparticles against cutaneous amyloidosis in rats: Histopathology and biochemical study |
| title_full_unstemmed | Ameliorative effect of zinc oxide nanoparticles against cutaneous amyloidosis in rats: Histopathology and biochemical study |
| title_short | Ameliorative effect of zinc oxide nanoparticles against cutaneous amyloidosis in rats: Histopathology and biochemical study |
| title_sort | ameliorative effect of zinc oxide nanoparticles against cutaneous amyloidosis in rats histopathology and biochemical study |
| topic | amyloidosis zinc oxide nanoparticle saa |
| url | https://www.vetmedmosul.com/article_188173_1c629ad690b2fc5e86d211e3ee6ce8fb.pdf |
| work_keys_str_mv | AT anfalasalaheldeen ameliorativeeffectofzincoxidenanoparticlesagainstcutaneousamyloidosisinratshistopathologyandbiochemicalstudy AT enassmustafa ameliorativeeffectofzincoxidenanoparticlesagainstcutaneousamyloidosisinratshistopathologyandbiochemicalstudy |