Fabrication of poly ( $$\varepsilon$$ -caprolactone) 3D scaffolds with controllable porosity using ultrasound

Abstract 3D printing has progressed significantly, allowing objects to be produced using a wide variety of materials. Recent advances have employed focused ultrasound in 3D printing, to allow printing inside acoustically transparent materials. Here we introduce a selective ultrasonic melting (SUM) m...

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Main Authors: Martin Weber, Dmitry Nikolaev, Mikko Koskenniemi, Jere Hyvönen, Joel Jääskeläinen, Armand Navarre, Ekaterina Takmakova, Arun Teotia, Pekka Katajisto, Robert Luxenhofer, Edward Hæggström, Ari Salmi
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-025-06818-9
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author Martin Weber
Dmitry Nikolaev
Mikko Koskenniemi
Jere Hyvönen
Joel Jääskeläinen
Armand Navarre
Ekaterina Takmakova
Arun Teotia
Pekka Katajisto
Robert Luxenhofer
Edward Hæggström
Ari Salmi
author_facet Martin Weber
Dmitry Nikolaev
Mikko Koskenniemi
Jere Hyvönen
Joel Jääskeläinen
Armand Navarre
Ekaterina Takmakova
Arun Teotia
Pekka Katajisto
Robert Luxenhofer
Edward Hæggström
Ari Salmi
author_sort Martin Weber
collection DOAJ
description Abstract 3D printing has progressed significantly, allowing objects to be produced using a wide variety of materials. Recent advances have employed focused ultrasound in 3D printing, to allow printing inside acoustically transparent materials. Here we introduce a selective ultrasonic melting (SUM) method for 3D printing of poly ( $$\varepsilon$$ -caprolactone) powder mixed with water. The printing was done by mechanically moving a focused ultrasound transducer. The microstructure and porosity of the prints were analyzed with micro-computed tomography. The open porosity of the printed samples was determined using the water intrusion method and by passing fluorescent microspheres through the structure. The cytocompatibility of the printed structures was confirmed by seeding NIH-3T3 fibroblast cells on the scaffolds, followed by analysis using live/dead fluorescent assay and visualization using scanning electron microscopy. We demonstrated that SUM is a viable technique to print structures with active control of their porosity. This method provides an alternative to methods such as fused deposition modelling and material jetting.
format Article
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institution Kabale University
issn 2045-2322
language English
publishDate 2025-07-01
publisher Nature Portfolio
record_format Article
series Scientific Reports
spelling doaj-art-d5cd035d83b34de085237add10f094952025-08-20T04:01:34ZengNature PortfolioScientific Reports2045-23222025-07-0115111210.1038/s41598-025-06818-9Fabrication of poly ( $$\varepsilon$$ -caprolactone) 3D scaffolds with controllable porosity using ultrasoundMartin Weber0Dmitry Nikolaev1Mikko Koskenniemi2Jere Hyvönen3Joel Jääskeläinen4Armand Navarre5Ekaterina Takmakova6Arun Teotia7Pekka Katajisto8Robert Luxenhofer9Edward Hæggström10Ari Salmi11Electronics Research Laboratory, Department of Physics, Faculty of Science, University of HelsinkiElectronics Research Laboratory, Department of Physics, Faculty of Science, University of HelsinkiElectronics Research Laboratory, Department of Physics, Faculty of Science, University of HelsinkiElectronics Research Laboratory, Department of Physics, Faculty of Science, University of HelsinkiElectronics Research Laboratory, Department of Physics, Faculty of Science, University of HelsinkiTechnical college of BloisSoft Matter Chemistry, Department of Chemistry, and Helsinki Institute of Sustainability Science, Faculty of Science, University of HelsinkiInstitute of Biotechnology (HiLIFE), Faculty of Biological and Environmental Sciences, University of HelsinkiInstitute of Biotechnology (HiLIFE), Faculty of Biological and Environmental Sciences, University of HelsinkiSoft Matter Chemistry, Department of Chemistry, and Helsinki Institute of Sustainability Science, Faculty of Science, University of HelsinkiElectronics Research Laboratory, Department of Physics, Faculty of Science, University of HelsinkiElectronics Research Laboratory, Department of Physics, Faculty of Science, University of HelsinkiAbstract 3D printing has progressed significantly, allowing objects to be produced using a wide variety of materials. Recent advances have employed focused ultrasound in 3D printing, to allow printing inside acoustically transparent materials. Here we introduce a selective ultrasonic melting (SUM) method for 3D printing of poly ( $$\varepsilon$$ -caprolactone) powder mixed with water. The printing was done by mechanically moving a focused ultrasound transducer. The microstructure and porosity of the prints were analyzed with micro-computed tomography. The open porosity of the printed samples was determined using the water intrusion method and by passing fluorescent microspheres through the structure. The cytocompatibility of the printed structures was confirmed by seeding NIH-3T3 fibroblast cells on the scaffolds, followed by analysis using live/dead fluorescent assay and visualization using scanning electron microscopy. We demonstrated that SUM is a viable technique to print structures with active control of their porosity. This method provides an alternative to methods such as fused deposition modelling and material jetting.https://doi.org/10.1038/s41598-025-06818-9
spellingShingle Martin Weber
Dmitry Nikolaev
Mikko Koskenniemi
Jere Hyvönen
Joel Jääskeläinen
Armand Navarre
Ekaterina Takmakova
Arun Teotia
Pekka Katajisto
Robert Luxenhofer
Edward Hæggström
Ari Salmi
Fabrication of poly ( $$\varepsilon$$ -caprolactone) 3D scaffolds with controllable porosity using ultrasound
Scientific Reports
title Fabrication of poly ( $$\varepsilon$$ -caprolactone) 3D scaffolds with controllable porosity using ultrasound
title_full Fabrication of poly ( $$\varepsilon$$ -caprolactone) 3D scaffolds with controllable porosity using ultrasound
title_fullStr Fabrication of poly ( $$\varepsilon$$ -caprolactone) 3D scaffolds with controllable porosity using ultrasound
title_full_unstemmed Fabrication of poly ( $$\varepsilon$$ -caprolactone) 3D scaffolds with controllable porosity using ultrasound
title_short Fabrication of poly ( $$\varepsilon$$ -caprolactone) 3D scaffolds with controllable porosity using ultrasound
title_sort fabrication of poly varepsilon caprolactone 3d scaffolds with controllable porosity using ultrasound
url https://doi.org/10.1038/s41598-025-06818-9
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