The Influence of Aging on the Regenerative Potential of Human Adipose Derived Mesenchymal Stem Cells
Tissue regeneration using human adipose derived mesenchymal stem cells (hASCs) has significant potential as a novel treatment for many degenerative bone and joint diseases. Previous studies have established that age negatively affects the proliferation status and the osteogenic and chondrogenic diff...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/2152435 |
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| author | Monika Marędziak Krzysztof Marycz Krzysztof A. Tomaszewski Katarzyna Kornicka Brandon Michael Henry |
| author_facet | Monika Marędziak Krzysztof Marycz Krzysztof A. Tomaszewski Katarzyna Kornicka Brandon Michael Henry |
| author_sort | Monika Marędziak |
| collection | DOAJ |
| description | Tissue regeneration using human adipose derived mesenchymal stem cells (hASCs) has significant potential as a novel treatment for many degenerative bone and joint diseases. Previous studies have established that age negatively affects the proliferation status and the osteogenic and chondrogenic differentiation potential of mesenchymal stem cells. The aim of this study was to assess the age-related maintenance of physiological function and differentiation potential of hASCs in vitro. hASCs were isolated from patients of four different age groups: (1) >20 years (n=7), (2) >50 years (n=7), (3) >60 years (n=7), and (4) >70 years (n=7). The hASCs were characterized according to the number of fibroblasts colony forming unit (CFU-F), proliferation rate, population doubling time (PDT), and quantified parameters of adipogenic, chondrogenic, and osteogenic differentiation. Compared to younger cells, aged hASCs had decreased proliferation rates, decreased chondrogenic and osteogenic potential, and increased senescent features. A shift in favor of adipogenic differentiation with increased age was also observed. As many bone and joint diseases increase in prevalence with age, it is important to consider the negative influence of age on hASCs viability, proliferation status, and multilineage differentiation potential when considering the potential therapeutic applications of hASCs. |
| format | Article |
| id | doaj-art-d5bca047bc6c4b669ef19c773f83ae53 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-d5bca047bc6c4b669ef19c773f83ae532025-02-03T06:06:13ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/21524352152435The Influence of Aging on the Regenerative Potential of Human Adipose Derived Mesenchymal Stem CellsMonika Marędziak0Krzysztof Marycz1Krzysztof A. Tomaszewski2Katarzyna Kornicka3Brandon Michael Henry4Department of Animal Physiology and Biostructure, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, 50-375 Wroclaw, PolandElectron Microscopy Laboratory, Wroclaw University of Environmental and Life Sciences, 50-631 Wroclaw, PolandDepartment of Anatomy, Jagiellonian University Medical College, 31-034 Krakow, PolandElectron Microscopy Laboratory, Wroclaw University of Environmental and Life Sciences, 50-631 Wroclaw, PolandDepartment of Anatomy, Jagiellonian University Medical College, 31-034 Krakow, PolandTissue regeneration using human adipose derived mesenchymal stem cells (hASCs) has significant potential as a novel treatment for many degenerative bone and joint diseases. Previous studies have established that age negatively affects the proliferation status and the osteogenic and chondrogenic differentiation potential of mesenchymal stem cells. The aim of this study was to assess the age-related maintenance of physiological function and differentiation potential of hASCs in vitro. hASCs were isolated from patients of four different age groups: (1) >20 years (n=7), (2) >50 years (n=7), (3) >60 years (n=7), and (4) >70 years (n=7). The hASCs were characterized according to the number of fibroblasts colony forming unit (CFU-F), proliferation rate, population doubling time (PDT), and quantified parameters of adipogenic, chondrogenic, and osteogenic differentiation. Compared to younger cells, aged hASCs had decreased proliferation rates, decreased chondrogenic and osteogenic potential, and increased senescent features. A shift in favor of adipogenic differentiation with increased age was also observed. As many bone and joint diseases increase in prevalence with age, it is important to consider the negative influence of age on hASCs viability, proliferation status, and multilineage differentiation potential when considering the potential therapeutic applications of hASCs.http://dx.doi.org/10.1155/2016/2152435 |
| spellingShingle | Monika Marędziak Krzysztof Marycz Krzysztof A. Tomaszewski Katarzyna Kornicka Brandon Michael Henry The Influence of Aging on the Regenerative Potential of Human Adipose Derived Mesenchymal Stem Cells Stem Cells International |
| title | The Influence of Aging on the Regenerative Potential of Human Adipose Derived Mesenchymal Stem Cells |
| title_full | The Influence of Aging on the Regenerative Potential of Human Adipose Derived Mesenchymal Stem Cells |
| title_fullStr | The Influence of Aging on the Regenerative Potential of Human Adipose Derived Mesenchymal Stem Cells |
| title_full_unstemmed | The Influence of Aging on the Regenerative Potential of Human Adipose Derived Mesenchymal Stem Cells |
| title_short | The Influence of Aging on the Regenerative Potential of Human Adipose Derived Mesenchymal Stem Cells |
| title_sort | influence of aging on the regenerative potential of human adipose derived mesenchymal stem cells |
| url | http://dx.doi.org/10.1155/2016/2152435 |
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