First report of the causal agent of vine crown gall in Mendoza, Argentina
Crown gall is one widespread grapevine disease worldwide, caused by Allorhizobium vitis (syn. Agrobacterium vitis) and Agrobacterium tumefaciens (syn. Rhizobium radiobacter). All. Vitis, is the predominant species and primary cause of the disease. This study aimed to identify and characterize mo...
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Facultad de Ciencias Agrarias. Universidad Nacional de Cuyo
2024-12-01
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| Series: | Revista de la Facultad de Ciencias Agrarias |
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| Online Access: | https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/7810 |
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| author | Sandra D’Innocenzo Mariano Emanuel Diaz Maria Georgina Escoriaza |
| author_facet | Sandra D’Innocenzo Mariano Emanuel Diaz Maria Georgina Escoriaza |
| author_sort | Sandra D’Innocenzo |
| collection | DOAJ |
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Crown gall is one widespread grapevine disease worldwide, caused by Allorhizobium vitis (syn. Agrobacterium vitis) and Agrobacterium tumefaciens (syn. Rhizobium radiobacter). All. Vitis, is the predominant species and primary cause of the disease. This study aimed to identify and characterize molecularly the agrobacteria in plants with crown gall symptoms in Mendoza vineyards. Diseased plants with trunk-based galls were sampled from different areas of Mendoza. Two multiplex PCRs were performed for bacterial identification and characterization, demonstrating that 91.6% of the strains obtained were agrobacteria (77% A. tumefaciens and 23% All. vitis). Fifty percent of All. vitis and 16% of A. tumefaciens identified strains were pathogenic. Pathogenicity tests were also conducted on Kalanchoe daigremontiana, with resulting tumorigenic symptoms.
Highlights:
• Crown gall is one widespread grapevine disease in the world, caused by Allorhizobium vitis (syn. Agrobacterium vitis) and Agrobacterium tumefaciens (syn. Rhizobium radiobacter).
• This study aimed to identify and characterize molecularly the causal agent of crown gall in Mendoza vineyards.
• One hundred and forty-eight symptomatic plants were collected from various vine-growing areas of Mendoza.
• Allorhizobium vitis and Agrobacterium tumefaciens strains were molecularly identified as pathogenic and octopine/nopaline type.
• All vitis is the predominant pathogenic species in grapevines studied in Mendoza.
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| format | Article |
| id | doaj-art-d5b8a2d1322940adaee4b72f01a1a3d2 |
| institution | DOAJ |
| issn | 0370-4661 1853-8665 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Facultad de Ciencias Agrarias. Universidad Nacional de Cuyo |
| record_format | Article |
| series | Revista de la Facultad de Ciencias Agrarias |
| spelling | doaj-art-d5b8a2d1322940adaee4b72f01a1a3d22025-08-20T02:50:56ZengFacultad de Ciencias Agrarias. Universidad Nacional de CuyoRevista de la Facultad de Ciencias Agrarias0370-46611853-86652024-12-0156210.48162/rev.39.139First report of the causal agent of vine crown gall in Mendoza, ArgentinaSandra D’Innocenzo0Mariano Emanuel Diaz1Maria Georgina Escoriaza2INTA EEA Mendoza. Laboratorio de Fitopatología. San Martín 3853. Luján de Cuyo. C. P. 5507INTA EEA Mendoza. Laboratorio de Fitopatología. San Martín 3853. Luján de Cuyo. C. P. 5507Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Cátedra de Industrias Agrarias FCA UNCuyo. Almirante Brown 500. M5528AHB. Chacras de Coria. Mendoza. Argentina Crown gall is one widespread grapevine disease worldwide, caused by Allorhizobium vitis (syn. Agrobacterium vitis) and Agrobacterium tumefaciens (syn. Rhizobium radiobacter). All. Vitis, is the predominant species and primary cause of the disease. This study aimed to identify and characterize molecularly the agrobacteria in plants with crown gall symptoms in Mendoza vineyards. Diseased plants with trunk-based galls were sampled from different areas of Mendoza. Two multiplex PCRs were performed for bacterial identification and characterization, demonstrating that 91.6% of the strains obtained were agrobacteria (77% A. tumefaciens and 23% All. vitis). Fifty percent of All. vitis and 16% of A. tumefaciens identified strains were pathogenic. Pathogenicity tests were also conducted on Kalanchoe daigremontiana, with resulting tumorigenic symptoms. Highlights: • Crown gall is one widespread grapevine disease in the world, caused by Allorhizobium vitis (syn. Agrobacterium vitis) and Agrobacterium tumefaciens (syn. Rhizobium radiobacter). • This study aimed to identify and characterize molecularly the causal agent of crown gall in Mendoza vineyards. • One hundred and forty-eight symptomatic plants were collected from various vine-growing areas of Mendoza. • Allorhizobium vitis and Agrobacterium tumefaciens strains were molecularly identified as pathogenic and octopine/nopaline type. • All vitis is the predominant pathogenic species in grapevines studied in Mendoza. https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/7810AllorhizobiumAgrobacteriumVitis viniferacrown gallMendoza |
| spellingShingle | Sandra D’Innocenzo Mariano Emanuel Diaz Maria Georgina Escoriaza First report of the causal agent of vine crown gall in Mendoza, Argentina Revista de la Facultad de Ciencias Agrarias Allorhizobium Agrobacterium Vitis vinifera crown gall Mendoza |
| title | First report of the causal agent of vine crown gall in Mendoza, Argentina |
| title_full | First report of the causal agent of vine crown gall in Mendoza, Argentina |
| title_fullStr | First report of the causal agent of vine crown gall in Mendoza, Argentina |
| title_full_unstemmed | First report of the causal agent of vine crown gall in Mendoza, Argentina |
| title_short | First report of the causal agent of vine crown gall in Mendoza, Argentina |
| title_sort | first report of the causal agent of vine crown gall in mendoza argentina |
| topic | Allorhizobium Agrobacterium Vitis vinifera crown gall Mendoza |
| url | https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/7810 |
| work_keys_str_mv | AT sandradinnocenzo firstreportofthecausalagentofvinecrowngallinmendozaargentina AT marianoemanueldiaz firstreportofthecausalagentofvinecrowngallinmendozaargentina AT mariageorginaescoriaza firstreportofthecausalagentofvinecrowngallinmendozaargentina |