A screening strategy identified LsNHL10 as an important regulator of grafting-enhanced cold tolerance in bottle gourd
Cold stress is one of the predominant abiotic constraints in the cultivation practices of vegetable crop. Bottle gourd [Lagenaria siceraria (Mol.) Standl.], a thermophilic Cucurbitaceae vegetable crop, exhibits significant sensitivity to cold stress. However, current researches remain relatively lim...
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Elsevier
2025-09-01
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| Series: | Plant Stress |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667064X25002106 |
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| author | Haiyan Wang Xiaohua Wu Xiao Guo Jian Wang Min Wang Xinyi Wu Baogen Wang Guojing Li Pei Xu Ying Wang |
| author_facet | Haiyan Wang Xiaohua Wu Xiao Guo Jian Wang Min Wang Xinyi Wu Baogen Wang Guojing Li Pei Xu Ying Wang |
| author_sort | Haiyan Wang |
| collection | DOAJ |
| description | Cold stress is one of the predominant abiotic constraints in the cultivation practices of vegetable crop. Bottle gourd [Lagenaria siceraria (Mol.) Standl.], a thermophilic Cucurbitaceae vegetable crop, exhibits significant sensitivity to cold stress. However, current researches remain relatively limited in elucidating the molecular mechanisms and physiological responses to cold stress in bottle gourd. Here, we analyzed the morphological and physiological indicators of heterografted bottle gourd-pumpkin seedlings (Ls/Cm) and allografted bottle gourd-bottle gourd seedlings (Ls/Ls) under cold stress (1 °C) at different time points (0, 3, 6, 9, 12 h). It was found that heterologous grafting significantly enhanced the cold stress tolerance of bottle gourd by more resistant phenotypes, higher maximal photochemical efficiency (Fv /Fm), lower malondialdehyde (MDA) content and relative electric conductivity (REC), greater increase in superoxide dismutase (SOD), peroxidase (POD) activity, and proline (PRO) content. Further, we developed a screening strategy for identifying cold stress-responsive genes in grafted bottle gourd seedings, and by applying this screening strategy, an important cold-stress candidate gene LsNHL10 was identified. Tissue-specific profiling using RT-qPCR revealed that LsNHL10 was abundantly expressed in young leaves and subcellular localization confirmed it was localized to cell nucleus, cytoplasm and chloroplast. The functional analysis demonstrated that transient overexpression of LsNHL10 enhanced the cold stress tolerance of bottle gourd, whereas transient silencing weakened that. Our study provided a screening strategy for cold stress-responsive gene identification in grafted bottle gourd and revealed the mechanistic basis that conferred adaptive benefits of bottle gourd plants under cold stress. |
| format | Article |
| id | doaj-art-d5b7e9bcf1914f44a75ba3239d792443 |
| institution | Kabale University |
| issn | 2667-064X |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Plant Stress |
| spelling | doaj-art-d5b7e9bcf1914f44a75ba3239d7924432025-08-23T04:49:34ZengElsevierPlant Stress2667-064X2025-09-011710094210.1016/j.stress.2025.100942A screening strategy identified LsNHL10 as an important regulator of grafting-enhanced cold tolerance in bottle gourdHaiyan Wang0Xiaohua Wu1Xiao Guo2Jian Wang3Min Wang4Xinyi Wu5Baogen Wang6Guojing Li7Pei Xu8Ying Wang9Institute of Vegetables, Zhejiang Academy of Agricultural Sciences/ Key Laboratory of Vegetable Germplasm Innovation and Quality Breeding in the Province, Hangzhou 310021, PR China; College of Life Sciences, China Jiliang University, Hangzhou 310018, PR ChinaInstitute of Vegetables, Zhejiang Academy of Agricultural Sciences/ Key Laboratory of Vegetable Germplasm Innovation and Quality Breeding in the Province, Hangzhou 310021, PR ChinaInstitute of Vegetables, Zhejiang Academy of Agricultural Sciences/ Key Laboratory of Vegetable Germplasm Innovation and Quality Breeding in the Province, Hangzhou 310021, PR ChinaInstitute of Vegetables, Zhejiang Academy of Agricultural Sciences/ Key Laboratory of Vegetable Germplasm Innovation and Quality Breeding in the Province, Hangzhou 310021, PR ChinaVegetable Research Institute, Guangdong Academy of Agricultural Sciences/ Guangdong Key Laboratory for New Technology Research of Vegetables, Guangzhou 510640, PR ChinaInstitute of Vegetables, Zhejiang Academy of Agricultural Sciences/ Key Laboratory of Vegetable Germplasm Innovation and Quality Breeding in the Province, Hangzhou 310021, PR ChinaInstitute of Vegetables, Zhejiang Academy of Agricultural Sciences/ Key Laboratory of Vegetable Germplasm Innovation and Quality Breeding in the Province, Hangzhou 310021, PR ChinaInstitute of Vegetables, Zhejiang Academy of Agricultural Sciences/ Key Laboratory of Vegetable Germplasm Innovation and Quality Breeding in the Province, Hangzhou 310021, PR ChinaCollege of Life Sciences, China Jiliang University, Hangzhou 310018, PR ChinaInstitute of Vegetables, Zhejiang Academy of Agricultural Sciences/ Key Laboratory of Vegetable Germplasm Innovation and Quality Breeding in the Province, Hangzhou 310021, PR China; State Key Laboratory for Quality and Safety of Agro-Products, Hangzhou 310021, PR China; Corresponding author.Cold stress is one of the predominant abiotic constraints in the cultivation practices of vegetable crop. Bottle gourd [Lagenaria siceraria (Mol.) Standl.], a thermophilic Cucurbitaceae vegetable crop, exhibits significant sensitivity to cold stress. However, current researches remain relatively limited in elucidating the molecular mechanisms and physiological responses to cold stress in bottle gourd. Here, we analyzed the morphological and physiological indicators of heterografted bottle gourd-pumpkin seedlings (Ls/Cm) and allografted bottle gourd-bottle gourd seedlings (Ls/Ls) under cold stress (1 °C) at different time points (0, 3, 6, 9, 12 h). It was found that heterologous grafting significantly enhanced the cold stress tolerance of bottle gourd by more resistant phenotypes, higher maximal photochemical efficiency (Fv /Fm), lower malondialdehyde (MDA) content and relative electric conductivity (REC), greater increase in superoxide dismutase (SOD), peroxidase (POD) activity, and proline (PRO) content. Further, we developed a screening strategy for identifying cold stress-responsive genes in grafted bottle gourd seedings, and by applying this screening strategy, an important cold-stress candidate gene LsNHL10 was identified. Tissue-specific profiling using RT-qPCR revealed that LsNHL10 was abundantly expressed in young leaves and subcellular localization confirmed it was localized to cell nucleus, cytoplasm and chloroplast. The functional analysis demonstrated that transient overexpression of LsNHL10 enhanced the cold stress tolerance of bottle gourd, whereas transient silencing weakened that. Our study provided a screening strategy for cold stress-responsive gene identification in grafted bottle gourd and revealed the mechanistic basis that conferred adaptive benefits of bottle gourd plants under cold stress.http://www.sciencedirect.com/science/article/pii/S2667064X25002106Cold stressBottle gourdLsNHL10 geneFunctional analysis |
| spellingShingle | Haiyan Wang Xiaohua Wu Xiao Guo Jian Wang Min Wang Xinyi Wu Baogen Wang Guojing Li Pei Xu Ying Wang A screening strategy identified LsNHL10 as an important regulator of grafting-enhanced cold tolerance in bottle gourd Plant Stress Cold stress Bottle gourd LsNHL10 gene Functional analysis |
| title | A screening strategy identified LsNHL10 as an important regulator of grafting-enhanced cold tolerance in bottle gourd |
| title_full | A screening strategy identified LsNHL10 as an important regulator of grafting-enhanced cold tolerance in bottle gourd |
| title_fullStr | A screening strategy identified LsNHL10 as an important regulator of grafting-enhanced cold tolerance in bottle gourd |
| title_full_unstemmed | A screening strategy identified LsNHL10 as an important regulator of grafting-enhanced cold tolerance in bottle gourd |
| title_short | A screening strategy identified LsNHL10 as an important regulator of grafting-enhanced cold tolerance in bottle gourd |
| title_sort | screening strategy identified lsnhl10 as an important regulator of grafting enhanced cold tolerance in bottle gourd |
| topic | Cold stress Bottle gourd LsNHL10 gene Functional analysis |
| url | http://www.sciencedirect.com/science/article/pii/S2667064X25002106 |
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