Harnessing intracellular bacteria in bladder by intravesical delivery of antibiotics-loaded nanodiamonds to reduce the recurrence of urinary tract infection
Abstract Treating urinary tract infections (UTIs) effectively is a difficult task due to the emergence of antibiotic-resistant bacteria and limited antibiotic access to intracellular bacteria within the bladder lining. Numerous studies of the antibiotics-nanodiamonds (NDs) synthesis and their inhibi...
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| Language: | English |
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BMC
2025-05-01
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| Series: | Journal of Nanobiotechnology |
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| Online Access: | https://doi.org/10.1186/s12951-025-03459-y |
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| author | Carmen Oi Kwan Law Hoi Man Leung Meijun Wang Qichang Nie Quynh Hoa Pham Kam Chu Han Tak Siu Wong Kwan Ting Chow Pik Kwan Lo Terrence Chi Kong Lau |
| author_facet | Carmen Oi Kwan Law Hoi Man Leung Meijun Wang Qichang Nie Quynh Hoa Pham Kam Chu Han Tak Siu Wong Kwan Ting Chow Pik Kwan Lo Terrence Chi Kong Lau |
| author_sort | Carmen Oi Kwan Law |
| collection | DOAJ |
| description | Abstract Treating urinary tract infections (UTIs) effectively is a difficult task due to the emergence of antibiotic-resistant bacteria and limited antibiotic access to intracellular bacteria within the bladder lining. Numerous studies of the antibiotics-nanodiamonds (NDs) synthesis and their inhibitory effect on bacteria have been performed previously. However, their effectiveness and toxicity in cell-based and animal infection models remain unclear. In this study, we presented the utilization of biopolymer-coated nanodiamonds for the delivery of tetracycline (TET) to the intracellular bacterial communities within the bladder cells using an intravesical delivery approach, aiming to effectively treat UTIs. Compared with antibiotics alone, the TET-loaded ND-based carrier system significantly improved the clearance of intracellular bacteria in the infected cell and animal models. Moreover, the intravesical delivery avoids the potential toxic effects from NDs accumulation in the organs, and minimizes the loss of the drugs during delivery. These results offer a promising strategy to treat chronic infections and prevent the recurrence of urinary tract infections (rUTIs). Graphical Abstract |
| format | Article |
| id | doaj-art-d5b412f229a74aca96d9dae822872fd4 |
| institution | OA Journals |
| issn | 1477-3155 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj-art-d5b412f229a74aca96d9dae822872fd42025-08-20T01:53:15ZengBMCJournal of Nanobiotechnology1477-31552025-05-0123111610.1186/s12951-025-03459-yHarnessing intracellular bacteria in bladder by intravesical delivery of antibiotics-loaded nanodiamonds to reduce the recurrence of urinary tract infectionCarmen Oi Kwan Law0Hoi Man Leung1Meijun Wang2Qichang Nie3Quynh Hoa Pham4Kam Chu Han5Tak Siu Wong6Kwan Ting Chow7Pik Kwan Lo8Terrence Chi Kong Lau9Department of Biomedical Sciences and Tung Biomedical Sciences Centre, City University of Hong KongDepartment of Chemistry and State Key Laboratory of Marine Pollution, City University of Hong KongDepartment of Biomedical Sciences and Tung Biomedical Sciences Centre, City University of Hong KongDepartment of Biomedical Sciences and Tung Biomedical Sciences Centre, City University of Hong KongDepartment of Biomedical Sciences and Tung Biomedical Sciences Centre, City University of Hong KongDepartment of Pathology, Pamela Youde Nethersole Eastern HospitalDepartment of Pathology, Pamela Youde Nethersole Eastern HospitalDepartment of Biomedical Sciences and Tung Biomedical Sciences Centre, City University of Hong KongDepartment of Chemistry and State Key Laboratory of Marine Pollution, City University of Hong KongDepartment of Biomedical Sciences and Tung Biomedical Sciences Centre, City University of Hong KongAbstract Treating urinary tract infections (UTIs) effectively is a difficult task due to the emergence of antibiotic-resistant bacteria and limited antibiotic access to intracellular bacteria within the bladder lining. Numerous studies of the antibiotics-nanodiamonds (NDs) synthesis and their inhibitory effect on bacteria have been performed previously. However, their effectiveness and toxicity in cell-based and animal infection models remain unclear. In this study, we presented the utilization of biopolymer-coated nanodiamonds for the delivery of tetracycline (TET) to the intracellular bacterial communities within the bladder cells using an intravesical delivery approach, aiming to effectively treat UTIs. Compared with antibiotics alone, the TET-loaded ND-based carrier system significantly improved the clearance of intracellular bacteria in the infected cell and animal models. Moreover, the intravesical delivery avoids the potential toxic effects from NDs accumulation in the organs, and minimizes the loss of the drugs during delivery. These results offer a promising strategy to treat chronic infections and prevent the recurrence of urinary tract infections (rUTIs). Graphical Abstracthttps://doi.org/10.1186/s12951-025-03459-yAntibacterial therapyAntibioticsIntravesical deliveryNanodiamondsUrinary tract infections |
| spellingShingle | Carmen Oi Kwan Law Hoi Man Leung Meijun Wang Qichang Nie Quynh Hoa Pham Kam Chu Han Tak Siu Wong Kwan Ting Chow Pik Kwan Lo Terrence Chi Kong Lau Harnessing intracellular bacteria in bladder by intravesical delivery of antibiotics-loaded nanodiamonds to reduce the recurrence of urinary tract infection Journal of Nanobiotechnology Antibacterial therapy Antibiotics Intravesical delivery Nanodiamonds Urinary tract infections |
| title | Harnessing intracellular bacteria in bladder by intravesical delivery of antibiotics-loaded nanodiamonds to reduce the recurrence of urinary tract infection |
| title_full | Harnessing intracellular bacteria in bladder by intravesical delivery of antibiotics-loaded nanodiamonds to reduce the recurrence of urinary tract infection |
| title_fullStr | Harnessing intracellular bacteria in bladder by intravesical delivery of antibiotics-loaded nanodiamonds to reduce the recurrence of urinary tract infection |
| title_full_unstemmed | Harnessing intracellular bacteria in bladder by intravesical delivery of antibiotics-loaded nanodiamonds to reduce the recurrence of urinary tract infection |
| title_short | Harnessing intracellular bacteria in bladder by intravesical delivery of antibiotics-loaded nanodiamonds to reduce the recurrence of urinary tract infection |
| title_sort | harnessing intracellular bacteria in bladder by intravesical delivery of antibiotics loaded nanodiamonds to reduce the recurrence of urinary tract infection |
| topic | Antibacterial therapy Antibiotics Intravesical delivery Nanodiamonds Urinary tract infections |
| url | https://doi.org/10.1186/s12951-025-03459-y |
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