DEVELOPMENT OF CYCLIC VOLTAMMETRIC METHOD FOR THE STUDY OF THE INTERACTION OF ANTIOXIDANT STANDARDS WITH SUPEROXIDE ANION RADICALS CASE OF α-TOCOPHEROL
This work aims to develop a cyclic voltammetry based assay for the calculation of binding parameters like binding constant, ratio of binding constants, and binding free energy of the free and superoxide anion radical bound forms of α-tocopherol (TOC) antioxidant standard. The determination of bindin...
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| Format: | Article |
| Language: | English |
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Alma Mater Publishing House "Vasile Alecsandri" University of Bacau
2015-07-01
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| Series: | Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry |
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| Online Access: | http://pubs.ub.ro/?pg=revues&rev=cscc6&num=201502&vol=2&aid=4235 |
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| _version_ | 1850198448900407296 |
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| author | TOUHAMI LANEZ MERIEM HENNI HADIA HEMMAMI |
| author_facet | TOUHAMI LANEZ MERIEM HENNI HADIA HEMMAMI |
| author_sort | TOUHAMI LANEZ |
| collection | DOAJ |
| description | This work aims to develop a cyclic voltammetry based assay for the calculation of binding parameters like binding constant, ratio of binding constants, and binding free energy of the free and superoxide anion radical bound forms of α-tocopherol (TOC) antioxidant standard. The determination of binding constant is based upon the decrease in anodic peak current of the redox couple in the presence of antioxidant standard, whereas the mode of interaction is concluded from the shift in peak potential values. The diffusion coefficients of both free superoxide anion radical and its bounded forms with TOC were also evaluated from Randles-Sevcik equation. |
| format | Article |
| id | doaj-art-d59a2cd2ab0742f2aefbd887943a5826 |
| institution | OA Journals |
| issn | 1582-540X 1582-540X |
| language | English |
| publishDate | 2015-07-01 |
| publisher | Alma Mater Publishing House "Vasile Alecsandri" University of Bacau |
| record_format | Article |
| series | Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry |
| spelling | doaj-art-d59a2cd2ab0742f2aefbd887943a58262025-08-20T02:12:53ZengAlma Mater Publishing House "Vasile Alecsandri" University of BacauScientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry1582-540X1582-540X2015-07-01162161168DEVELOPMENT OF CYCLIC VOLTAMMETRIC METHOD FOR THE STUDY OF THE INTERACTION OF ANTIOXIDANT STANDARDS WITH SUPEROXIDE ANION RADICALS CASE OF α-TOCOPHEROLTOUHAMI LANEZ0MERIEM HENNI1HADIA HEMMAMI2University of El Oued, VTRS Laboratory, PO Box 789, 39000, El Oued, AlgeriaUniversity of El Oued, VTRS Laboratory, PO Box 789, 39000, El Oued, AlgeriaUniversity of El Oued, VTRS Laboratory, PO Box 789, 39000, El Oued, AlgeriaThis work aims to develop a cyclic voltammetry based assay for the calculation of binding parameters like binding constant, ratio of binding constants, and binding free energy of the free and superoxide anion radical bound forms of α-tocopherol (TOC) antioxidant standard. The determination of binding constant is based upon the decrease in anodic peak current of the redox couple in the presence of antioxidant standard, whereas the mode of interaction is concluded from the shift in peak potential values. The diffusion coefficients of both free superoxide anion radical and its bounded forms with TOC were also evaluated from Randles-Sevcik equation.http://pubs.ub.ro/?pg=revues&rev=cscc6&num=201502&vol=2&aid=4235binding constantscommercial oxygendiffusion coefficientsfree energyRandles-Sevcik equation |
| spellingShingle | TOUHAMI LANEZ MERIEM HENNI HADIA HEMMAMI DEVELOPMENT OF CYCLIC VOLTAMMETRIC METHOD FOR THE STUDY OF THE INTERACTION OF ANTIOXIDANT STANDARDS WITH SUPEROXIDE ANION RADICALS CASE OF α-TOCOPHEROL Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry binding constants commercial oxygen diffusion coefficients free energy Randles-Sevcik equation |
| title | DEVELOPMENT OF CYCLIC VOLTAMMETRIC METHOD FOR THE STUDY OF THE INTERACTION OF ANTIOXIDANT STANDARDS WITH SUPEROXIDE ANION RADICALS CASE OF α-TOCOPHEROL |
| title_full | DEVELOPMENT OF CYCLIC VOLTAMMETRIC METHOD FOR THE STUDY OF THE INTERACTION OF ANTIOXIDANT STANDARDS WITH SUPEROXIDE ANION RADICALS CASE OF α-TOCOPHEROL |
| title_fullStr | DEVELOPMENT OF CYCLIC VOLTAMMETRIC METHOD FOR THE STUDY OF THE INTERACTION OF ANTIOXIDANT STANDARDS WITH SUPEROXIDE ANION RADICALS CASE OF α-TOCOPHEROL |
| title_full_unstemmed | DEVELOPMENT OF CYCLIC VOLTAMMETRIC METHOD FOR THE STUDY OF THE INTERACTION OF ANTIOXIDANT STANDARDS WITH SUPEROXIDE ANION RADICALS CASE OF α-TOCOPHEROL |
| title_short | DEVELOPMENT OF CYCLIC VOLTAMMETRIC METHOD FOR THE STUDY OF THE INTERACTION OF ANTIOXIDANT STANDARDS WITH SUPEROXIDE ANION RADICALS CASE OF α-TOCOPHEROL |
| title_sort | development of cyclic voltammetric method for the study of the interaction of antioxidant standards with superoxide anion radicals case of α tocopherol |
| topic | binding constants commercial oxygen diffusion coefficients free energy Randles-Sevcik equation |
| url | http://pubs.ub.ro/?pg=revues&rev=cscc6&num=201502&vol=2&aid=4235 |
| work_keys_str_mv | AT touhamilanez developmentofcyclicvoltammetricmethodforthestudyoftheinteractionofantioxidantstandardswithsuperoxideanionradicalscaseofatocopherol AT meriemhenni developmentofcyclicvoltammetricmethodforthestudyoftheinteractionofantioxidantstandardswithsuperoxideanionradicalscaseofatocopherol AT hadiahemmami developmentofcyclicvoltammetricmethodforthestudyoftheinteractionofantioxidantstandardswithsuperoxideanionradicalscaseofatocopherol |