Role of Paricalcitol in Modulating the Immune Response in Patients with Renal Disease
Introduction. The aim was to highlight the existence of a relationship between vitamin D deficiency, chronic inflammation, and proteinuria, by measuring neutrophil gelatinase associated lipocalin (NGAL) and common inflammatory markers after administration of paricalcitol, a vitamin D analog, in viv...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2015/765364 |
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| author | Silvia Lucisano Adriana Arena Giovanna Stassi Daniela Iannello Gaetano Montalto Adolfo Romeo Giuseppe Costantino Rosaria Lupica Valeria Cernaro Domenico Santoro Michele Buemi |
| author_facet | Silvia Lucisano Adriana Arena Giovanna Stassi Daniela Iannello Gaetano Montalto Adolfo Romeo Giuseppe Costantino Rosaria Lupica Valeria Cernaro Domenico Santoro Michele Buemi |
| author_sort | Silvia Lucisano |
| collection | DOAJ |
| description | Introduction. The aim was to highlight the existence of a relationship between vitamin D deficiency, chronic inflammation, and proteinuria, by measuring neutrophil gelatinase associated lipocalin (NGAL) and common inflammatory markers after administration of paricalcitol, a vitamin D analog, in vivo and in vitro. Methods. 40 patients with end-stage chronic kidney disease (CKD) and secondary hyperparathyroidism and 40 healthy subjects were enrolled. Serum calcium, phosphorus, 25(OH)-vitamin D, parathyroid hormone (PTH), erythrocyte sedimentation rate, high-sensitivity C-reactive protein, interleukin- (IL-) 17, IL-6, IL-1β, interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α), plasmatic and urinary NGAL, and 24 h albuminuria and proteinuria were measured before and 24 h after an intravenous bolus of paricalcitol (5 mcg). Human peripheral blood mononuclear cells were isolated and stimulated with phytohaemagglutinin. NGAL, IL-1β, IL-17, IL-6, TNF-α, and IFN-γ were measured in the culture medium and in the 24 h urine collection. Results. 25(OH)-vitamin D was lower in CKD than in controls (p<0.0001), while inflammatory markers were higher in CKD group (p<0.0001). In vivo and in vitro studies showed a downregulation of NGAL, IL-17, IL-6, IL-1β, TNF-α, and IFN-γ after paricalcitol administration (p<0.0001). Conclusions. 25(OH)-vitamin D regulates immune and inflammatory processes. Further studies are needed to confirm these data in order to improve the treatment of CKD patients. |
| format | Article |
| id | doaj-art-d594a66179884b02b99ade417ff3e11f |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-d594a66179884b02b99ade417ff3e11f2025-02-03T05:58:50ZengWileyInternational Journal of Endocrinology1687-83371687-83452015-01-01201510.1155/2015/765364765364Role of Paricalcitol in Modulating the Immune Response in Patients with Renal DiseaseSilvia Lucisano0Adriana Arena1Giovanna Stassi2Daniela Iannello3Gaetano Montalto4Adolfo Romeo5Giuseppe Costantino6Rosaria Lupica7Valeria Cernaro8Domenico Santoro9Michele Buemi10Chair of Nephrology, Department of Clinical and Experimental Medicine, University of Messina, 98124 Messina, ItalyDepartment of Human Pathology, Unit of Clinical Microbiology, University of Messina, 98124 Messina, ItalyDepartment of Human Pathology, Unit of Clinical Microbiology, University of Messina, 98124 Messina, ItalyDepartment of Human Pathology, Unit of Clinical Microbiology, University of Messina, 98124 Messina, ItalyChair of Nephrology, Department of Clinical and Experimental Medicine, University of Messina, 98124 Messina, ItalyChair of Nephrology, Department of Clinical and Experimental Medicine, University of Messina, 98124 Messina, ItalyChair of Nephrology, Department of Clinical and Experimental Medicine, University of Messina, 98124 Messina, ItalyChair of Nephrology, Department of Clinical and Experimental Medicine, University of Messina, 98124 Messina, ItalyChair of Nephrology, Department of Clinical and Experimental Medicine, University of Messina, 98124 Messina, ItalyChair of Nephrology, Department of Clinical and Experimental Medicine, University of Messina, 98124 Messina, ItalyChair of Nephrology, Department of Clinical and Experimental Medicine, University of Messina, 98124 Messina, ItalyIntroduction. The aim was to highlight the existence of a relationship between vitamin D deficiency, chronic inflammation, and proteinuria, by measuring neutrophil gelatinase associated lipocalin (NGAL) and common inflammatory markers after administration of paricalcitol, a vitamin D analog, in vivo and in vitro. Methods. 40 patients with end-stage chronic kidney disease (CKD) and secondary hyperparathyroidism and 40 healthy subjects were enrolled. Serum calcium, phosphorus, 25(OH)-vitamin D, parathyroid hormone (PTH), erythrocyte sedimentation rate, high-sensitivity C-reactive protein, interleukin- (IL-) 17, IL-6, IL-1β, interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α), plasmatic and urinary NGAL, and 24 h albuminuria and proteinuria were measured before and 24 h after an intravenous bolus of paricalcitol (5 mcg). Human peripheral blood mononuclear cells were isolated and stimulated with phytohaemagglutinin. NGAL, IL-1β, IL-17, IL-6, TNF-α, and IFN-γ were measured in the culture medium and in the 24 h urine collection. Results. 25(OH)-vitamin D was lower in CKD than in controls (p<0.0001), while inflammatory markers were higher in CKD group (p<0.0001). In vivo and in vitro studies showed a downregulation of NGAL, IL-17, IL-6, IL-1β, TNF-α, and IFN-γ after paricalcitol administration (p<0.0001). Conclusions. 25(OH)-vitamin D regulates immune and inflammatory processes. Further studies are needed to confirm these data in order to improve the treatment of CKD patients.http://dx.doi.org/10.1155/2015/765364 |
| spellingShingle | Silvia Lucisano Adriana Arena Giovanna Stassi Daniela Iannello Gaetano Montalto Adolfo Romeo Giuseppe Costantino Rosaria Lupica Valeria Cernaro Domenico Santoro Michele Buemi Role of Paricalcitol in Modulating the Immune Response in Patients with Renal Disease International Journal of Endocrinology |
| title | Role of Paricalcitol in Modulating the Immune Response in Patients with Renal Disease |
| title_full | Role of Paricalcitol in Modulating the Immune Response in Patients with Renal Disease |
| title_fullStr | Role of Paricalcitol in Modulating the Immune Response in Patients with Renal Disease |
| title_full_unstemmed | Role of Paricalcitol in Modulating the Immune Response in Patients with Renal Disease |
| title_short | Role of Paricalcitol in Modulating the Immune Response in Patients with Renal Disease |
| title_sort | role of paricalcitol in modulating the immune response in patients with renal disease |
| url | http://dx.doi.org/10.1155/2015/765364 |
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