Identification and validation of PANoptosis-related LncRNAs prognosis system in hepatocellular carcinoma
Abstract Hepatocellular carcinoma (HCC) is one of the most common solid malignancies in the world. Due to the limited effectiveness of current drug treatments, further research on HCC is necessary. PANoptosis is defined as an inflammatory RCD whose main features combine pyroptosis, apoptosis and nec...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-02-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-90498-y |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849723975462027264 |
|---|---|
| author | Qi Shu Junfeng Zhu Jiaping Mo Xiaoyan Wei Zhenjie Zhu Xiaojuan Chen Fugen He Like Zhong |
| author_facet | Qi Shu Junfeng Zhu Jiaping Mo Xiaoyan Wei Zhenjie Zhu Xiaojuan Chen Fugen He Like Zhong |
| author_sort | Qi Shu |
| collection | DOAJ |
| description | Abstract Hepatocellular carcinoma (HCC) is one of the most common solid malignancies in the world. Due to the limited effectiveness of current drug treatments, further research on HCC is necessary. PANoptosis is defined as an inflammatory RCD whose main features combine pyroptosis, apoptosis and necroptosis which cannot be explained by any of these three RCDs alone. In HCC, risk stratification based on PANoptosis-associated lncRNAs has clinical application potential. In this study, we explored HCC related PANoptosis-related lncRNAs (PRLs) by analyzing significantly differentially expressed genes in HCC. HCC-associated PRL scores were established by WGCNA, LASSO analysis and multivariate Cox assessment. Subsequently, we verified the prognostic analysis ability of PRL score for HCC patients, and on this basis established a prognostic risk assessment model for HCC and verified its reliability. The relationship between PRL score and immune infiltration as well as drug sensitivity was further analyzed to evaluate the clinical reference value of this model. Western blot analysis and PCR further verified the reliability of bioinformatics results. The observed suppression of HCC progression and invasiveness following selected PRL knockdown further validated the reliability of our bioinformatics analysis results. Our results provide new evidence for the role of PANoptosis-associated lncRNAs in HCC. |
| format | Article |
| id | doaj-art-d587a01b4fbd4c8da06d03d2d2a85a5e |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-d587a01b4fbd4c8da06d03d2d2a85a5e2025-08-20T03:10:53ZengNature PortfolioScientific Reports2045-23222025-02-0115111610.1038/s41598-025-90498-yIdentification and validation of PANoptosis-related LncRNAs prognosis system in hepatocellular carcinomaQi Shu0Junfeng Zhu1Jiaping Mo2Xiaoyan Wei3Zhenjie Zhu4Xiaojuan Chen5Fugen He6Like Zhong7Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of SciencesZhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of SciencesZhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of SciencesZhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of SciencesZhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of SciencesZhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of SciencesZhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of SciencesZhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of SciencesAbstract Hepatocellular carcinoma (HCC) is one of the most common solid malignancies in the world. Due to the limited effectiveness of current drug treatments, further research on HCC is necessary. PANoptosis is defined as an inflammatory RCD whose main features combine pyroptosis, apoptosis and necroptosis which cannot be explained by any of these three RCDs alone. In HCC, risk stratification based on PANoptosis-associated lncRNAs has clinical application potential. In this study, we explored HCC related PANoptosis-related lncRNAs (PRLs) by analyzing significantly differentially expressed genes in HCC. HCC-associated PRL scores were established by WGCNA, LASSO analysis and multivariate Cox assessment. Subsequently, we verified the prognostic analysis ability of PRL score for HCC patients, and on this basis established a prognostic risk assessment model for HCC and verified its reliability. The relationship between PRL score and immune infiltration as well as drug sensitivity was further analyzed to evaluate the clinical reference value of this model. Western blot analysis and PCR further verified the reliability of bioinformatics results. The observed suppression of HCC progression and invasiveness following selected PRL knockdown further validated the reliability of our bioinformatics analysis results. Our results provide new evidence for the role of PANoptosis-associated lncRNAs in HCC.https://doi.org/10.1038/s41598-025-90498-yHepatocellular carcinomaPANoptosislncRNAImmune infiltrationRisk stratificationPrognosis |
| spellingShingle | Qi Shu Junfeng Zhu Jiaping Mo Xiaoyan Wei Zhenjie Zhu Xiaojuan Chen Fugen He Like Zhong Identification and validation of PANoptosis-related LncRNAs prognosis system in hepatocellular carcinoma Scientific Reports Hepatocellular carcinoma PANoptosis lncRNA Immune infiltration Risk stratification Prognosis |
| title | Identification and validation of PANoptosis-related LncRNAs prognosis system in hepatocellular carcinoma |
| title_full | Identification and validation of PANoptosis-related LncRNAs prognosis system in hepatocellular carcinoma |
| title_fullStr | Identification and validation of PANoptosis-related LncRNAs prognosis system in hepatocellular carcinoma |
| title_full_unstemmed | Identification and validation of PANoptosis-related LncRNAs prognosis system in hepatocellular carcinoma |
| title_short | Identification and validation of PANoptosis-related LncRNAs prognosis system in hepatocellular carcinoma |
| title_sort | identification and validation of panoptosis related lncrnas prognosis system in hepatocellular carcinoma |
| topic | Hepatocellular carcinoma PANoptosis lncRNA Immune infiltration Risk stratification Prognosis |
| url | https://doi.org/10.1038/s41598-025-90498-y |
| work_keys_str_mv | AT qishu identificationandvalidationofpanoptosisrelatedlncrnasprognosissysteminhepatocellularcarcinoma AT junfengzhu identificationandvalidationofpanoptosisrelatedlncrnasprognosissysteminhepatocellularcarcinoma AT jiapingmo identificationandvalidationofpanoptosisrelatedlncrnasprognosissysteminhepatocellularcarcinoma AT xiaoyanwei identificationandvalidationofpanoptosisrelatedlncrnasprognosissysteminhepatocellularcarcinoma AT zhenjiezhu identificationandvalidationofpanoptosisrelatedlncrnasprognosissysteminhepatocellularcarcinoma AT xiaojuanchen identificationandvalidationofpanoptosisrelatedlncrnasprognosissysteminhepatocellularcarcinoma AT fugenhe identificationandvalidationofpanoptosisrelatedlncrnasprognosissysteminhepatocellularcarcinoma AT likezhong identificationandvalidationofpanoptosisrelatedlncrnasprognosissysteminhepatocellularcarcinoma |