Flavonoid Production in Callus Cultures from Mesocarp of Stelechocarpus burahol

<p><em>Stelechocarpus burahol </em>is one of the medicinal plants that contains flavonoids. The study was carried out to know flavonoid production of cultures in vitro <em>S. burahol </em>from mesocarp explants. Mesocarp explants were cultured on MS medium containing di...

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Main Authors: Noor Aini Habibah, Sukarti Moeljopawiro, Kumala Dewi, Ari Indrianto
Format: Article
Language:English
Published: Universitas Negeri Semarang 2016-09-01
Series:Biosaintifika: Journal of Biology & Biology Education
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Online Access:https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/6632
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author Noor Aini Habibah
Sukarti Moeljopawiro
Kumala Dewi
Ari Indrianto
author_facet Noor Aini Habibah
Sukarti Moeljopawiro
Kumala Dewi
Ari Indrianto
author_sort Noor Aini Habibah
collection DOAJ
description <p><em>Stelechocarpus burahol </em>is one of the medicinal plants that contains flavonoids. The study was carried out to know flavonoid production of cultures in vitro <em>S. burahol </em>from mesocarp explants. Mesocarp explants were cultured on MS medium containing different combination and concentration of plant growth regulators i.e. picloram (5, 7.5 and 10 mg/L) and 2, 4-D (10, 15 and 20 mg/L) under dark condition. Induction of callus formation started on the 20.29<sup>th</sup> to the 29.86<sup>th</sup> days. Medium supplemented with Picloram and dark state proved to be the best condition for optimum callus induction from mesocarp explants of <em>S. burahol</em>. Callus grown on medium with the addition of 7.5 mg/l Picloram produces the highest flavonoid. The maximum production of the secondary metabolite was obtained from 8 weeks old callus. However, by the time of callus ageing, its output has declined. It could be concluded that callus cultures from mesocarp <em>S. burahol</em> can be used for flavonoid production. </p><p><strong>How to Cite</strong></p><p>Habibah, N. A., Moeljopawiro, S. Dewi, K. &amp; Indrianto, A. (2016). Flavonoid Production in Callus Cultures from Mesocarp of <em>Stelechocarpus burahol</em>. <em>Biosaintifika: Journal of Biology &amp; Biology Education</em>, 8(2), 214-221.</p>
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issn 2085-191X
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publishDate 2016-09-01
publisher Universitas Negeri Semarang
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series Biosaintifika: Journal of Biology & Biology Education
spelling doaj-art-d5859217cc444c5492a013a4f3aed9f32025-08-20T03:19:33ZengUniversitas Negeri SemarangBiosaintifika: Journal of Biology & Biology Education2085-191X2338-76102016-09-018221422110.15294/biosaintifika.v8i2.66324860Flavonoid Production in Callus Cultures from Mesocarp of Stelechocarpus buraholNoor Aini Habibah0Sukarti Moeljopawiro1Kumala Dewi2Ari Indrianto3Doctorate Program of Faculty of Biology, Gadjah Mada University, IndonesiaFaculty of Biology, Gadjah Mada UniversityFaculty of Biology, Gadjah Mada UniversityFaculty of Biology, Gadjah Mada University<p><em>Stelechocarpus burahol </em>is one of the medicinal plants that contains flavonoids. The study was carried out to know flavonoid production of cultures in vitro <em>S. burahol </em>from mesocarp explants. Mesocarp explants were cultured on MS medium containing different combination and concentration of plant growth regulators i.e. picloram (5, 7.5 and 10 mg/L) and 2, 4-D (10, 15 and 20 mg/L) under dark condition. Induction of callus formation started on the 20.29<sup>th</sup> to the 29.86<sup>th</sup> days. Medium supplemented with Picloram and dark state proved to be the best condition for optimum callus induction from mesocarp explants of <em>S. burahol</em>. Callus grown on medium with the addition of 7.5 mg/l Picloram produces the highest flavonoid. The maximum production of the secondary metabolite was obtained from 8 weeks old callus. However, by the time of callus ageing, its output has declined. It could be concluded that callus cultures from mesocarp <em>S. burahol</em> can be used for flavonoid production. </p><p><strong>How to Cite</strong></p><p>Habibah, N. A., Moeljopawiro, S. Dewi, K. &amp; Indrianto, A. (2016). Flavonoid Production in Callus Cultures from Mesocarp of <em>Stelechocarpus burahol</em>. <em>Biosaintifika: Journal of Biology &amp; Biology Education</em>, 8(2), 214-221.</p>https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/6632mesocarpcallusStelechocarpus buraholflavonoid
spellingShingle Noor Aini Habibah
Sukarti Moeljopawiro
Kumala Dewi
Ari Indrianto
Flavonoid Production in Callus Cultures from Mesocarp of Stelechocarpus burahol
Biosaintifika: Journal of Biology & Biology Education
mesocarp
callus
Stelechocarpus burahol
flavonoid
title Flavonoid Production in Callus Cultures from Mesocarp of Stelechocarpus burahol
title_full Flavonoid Production in Callus Cultures from Mesocarp of Stelechocarpus burahol
title_fullStr Flavonoid Production in Callus Cultures from Mesocarp of Stelechocarpus burahol
title_full_unstemmed Flavonoid Production in Callus Cultures from Mesocarp of Stelechocarpus burahol
title_short Flavonoid Production in Callus Cultures from Mesocarp of Stelechocarpus burahol
title_sort flavonoid production in callus cultures from mesocarp of stelechocarpus burahol
topic mesocarp
callus
Stelechocarpus burahol
flavonoid
url https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/6632
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AT kumaladewi flavonoidproductionincallusculturesfrommesocarpofstelechocarpusburahol
AT ariindrianto flavonoidproductionincallusculturesfrommesocarpofstelechocarpusburahol