Effect of Shentongzhuyu Decoction on p38MAPK Signal Pathway in Annulus Fibrosus Cells of Model Rats with Lumbar Disc Degeneration

Objective:To explore the effect of Shentongzhuyu decoction on p38MAPK signal pathway in annulus fibrosus cells of model rats with lumbar disc degeneration.Methods:A total of 24 male adult SD rats were randomly divided into Shentongzhuyu decoction group, model group and sham-operated group. Models of...

Full description

Saved in:
Bibliographic Details
Main Authors: Sha CHEN, Shizhong WANG, Dewan DENG
Format: Article
Language:English
Published: Editorial Office of Rehabilitation Medicine 2015-12-01
Series:康复学报
Subjects:
Online Access:http://kfxb.publish.founderss.cn/thesisDetails#10.13261/j.issn.2096-0328.2015.04.006
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1841536973118898176
author Sha CHEN
Shizhong WANG
Dewan DENG
author_facet Sha CHEN
Shizhong WANG
Dewan DENG
author_sort Sha CHEN
collection DOAJ
description Objective:To explore the effect of Shentongzhuyu decoction on p38MAPK signal pathway in annulus fibrosus cells of model rats with lumbar disc degeneration.Methods:A total of 24 male adult SD rats were randomly divided into Shentongzhuyu decoction group, model group and sham-operated group. Models of lumbar disc degeneration were established in rats of Shentongzhuyu decoction group and model group with the method of puncturing the anulus fibrosus with needles. Then after one month, Shentongzhuyu decoction group received Shentongzhuyu decoction by gavage, and model group and sham-operated group received normal saline once daily for four weeks. After the treatment, the rats were killed and lumbar disc tissues were taken out. The protein expressions of p-p38, p38 and NF-κB were observed and detected by Immunohistochemistry and Western Blot.Results:The protein expressions of p-p38 and NF-κB in Shentongzhuyu decoction group were lower than those of model group respectively (<italic>P</italic>&lt;0.05), and the protein expressions of p-p38 and NF-κB in model group were higher than those of sham-operated group respectively (<italic>P</italic>&lt;0.01). The protein expressions of p-p38 and NF-κB in sham-operated group were not apparent.Conclusion:Shentongzhuyu decoction can delay lumbar disc degeneration, and the probalble mechanism is related to down-regulating the expression of p-p38 and NF-kB and inhibiting the activation of p38MAPK signal pathway.
format Article
id doaj-art-d57e43badcc24d5292923c74d94c2d46
institution Kabale University
issn 2096-0328
language English
publishDate 2015-12-01
publisher Editorial Office of Rehabilitation Medicine
record_format Article
series 康复学报
spelling doaj-art-d57e43badcc24d5292923c74d94c2d462025-01-14T10:06:37ZengEditorial Office of Rehabilitation Medicine康复学报2096-03282015-12-0125222623094947Effect of Shentongzhuyu Decoction on p38MAPK Signal Pathway in Annulus Fibrosus Cells of Model Rats with Lumbar Disc DegenerationSha CHENShizhong WANGDewan DENGObjective:To explore the effect of Shentongzhuyu decoction on p38MAPK signal pathway in annulus fibrosus cells of model rats with lumbar disc degeneration.Methods:A total of 24 male adult SD rats were randomly divided into Shentongzhuyu decoction group, model group and sham-operated group. Models of lumbar disc degeneration were established in rats of Shentongzhuyu decoction group and model group with the method of puncturing the anulus fibrosus with needles. Then after one month, Shentongzhuyu decoction group received Shentongzhuyu decoction by gavage, and model group and sham-operated group received normal saline once daily for four weeks. After the treatment, the rats were killed and lumbar disc tissues were taken out. The protein expressions of p-p38, p38 and NF-κB were observed and detected by Immunohistochemistry and Western Blot.Results:The protein expressions of p-p38 and NF-κB in Shentongzhuyu decoction group were lower than those of model group respectively (<italic>P</italic>&lt;0.05), and the protein expressions of p-p38 and NF-κB in model group were higher than those of sham-operated group respectively (<italic>P</italic>&lt;0.01). The protein expressions of p-p38 and NF-κB in sham-operated group were not apparent.Conclusion:Shentongzhuyu decoction can delay lumbar disc degeneration, and the probalble mechanism is related to down-regulating the expression of p-p38 and NF-kB and inhibiting the activation of p38MAPK signal pathway.http://kfxb.publish.founderss.cn/thesisDetails#10.13261/j.issn.2096-0328.2015.04.006Shentongzhuyu decoctionintervebral disc degenerationannulus fibrosusp-p38p38NF-κBp38MAPK
spellingShingle Sha CHEN
Shizhong WANG
Dewan DENG
Effect of Shentongzhuyu Decoction on p38MAPK Signal Pathway in Annulus Fibrosus Cells of Model Rats with Lumbar Disc Degeneration
康复学报
Shentongzhuyu decoction
intervebral disc degeneration
annulus fibrosus
p-p38
p38
NF-κB
p38MAPK
title Effect of Shentongzhuyu Decoction on p38MAPK Signal Pathway in Annulus Fibrosus Cells of Model Rats with Lumbar Disc Degeneration
title_full Effect of Shentongzhuyu Decoction on p38MAPK Signal Pathway in Annulus Fibrosus Cells of Model Rats with Lumbar Disc Degeneration
title_fullStr Effect of Shentongzhuyu Decoction on p38MAPK Signal Pathway in Annulus Fibrosus Cells of Model Rats with Lumbar Disc Degeneration
title_full_unstemmed Effect of Shentongzhuyu Decoction on p38MAPK Signal Pathway in Annulus Fibrosus Cells of Model Rats with Lumbar Disc Degeneration
title_short Effect of Shentongzhuyu Decoction on p38MAPK Signal Pathway in Annulus Fibrosus Cells of Model Rats with Lumbar Disc Degeneration
title_sort effect of shentongzhuyu decoction on p38mapk signal pathway in annulus fibrosus cells of model rats with lumbar disc degeneration
topic Shentongzhuyu decoction
intervebral disc degeneration
annulus fibrosus
p-p38
p38
NF-κB
p38MAPK
url http://kfxb.publish.founderss.cn/thesisDetails#10.13261/j.issn.2096-0328.2015.04.006
work_keys_str_mv AT shachen effectofshentongzhuyudecoctiononp38mapksignalpathwayinannulusfibrosuscellsofmodelratswithlumbardiscdegeneration
AT shizhongwang effectofshentongzhuyudecoctiononp38mapksignalpathwayinannulusfibrosuscellsofmodelratswithlumbardiscdegeneration
AT dewandeng effectofshentongzhuyudecoctiononp38mapksignalpathwayinannulusfibrosuscellsofmodelratswithlumbardiscdegeneration