Effects of Tremella fuciformis -Derived Polysaccharides with Different Molecular Weight on D-Galactose-Induced Aging of Mice
The structure-bioactivity relationship of Tremella fuciformis polysaccharides (TFPs) in anti-aging in vivo is rarely reported. In the present study, a purified TFP, named HM, mainly composed of mannose, fucose, xylose, and glucose in a molar ratio of 4.14:0.98:0.81:0.62, was obtained from the fr...
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Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences
2023-05-01
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| Series: | Polish Journal of Food and Nutrition Sciences |
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| Online Access: | http://journal.pan.olsztyn.pl/Effects-of-Tremella-fuciformis-Derived-Polysaccharides-with-Different-Molecular-Weight,163612,0,2.html |
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| author | Donghui Luo Xiaofei Liu Jingjing Guan Guili Jang Yanglin Hua Xinfei Zhang Xiaofei Xu |
| author_facet | Donghui Luo Xiaofei Liu Jingjing Guan Guili Jang Yanglin Hua Xinfei Zhang Xiaofei Xu |
| author_sort | Donghui Luo |
| collection | DOAJ |
| description | The structure-bioactivity relationship of Tremella fuciformis polysaccharides (TFPs) in anti-aging in vivo is rarely reported. In the present study, a purified TFP, named HM, mainly composed of mannose, fucose, xylose, and glucose in a molar ratio of 4.14:0.98:0.81:0.62, was obtained from the fruiting body of T. fuciformis . Subsequently, two differentially degraded TFPs, named MM and LM, respectively, were prepared by a combined method of ultrasonic irradiation (US) and H 2 O 2 treatment. Their structural properties, scavenging activities against free radicals in vitro , and anti-aging effects on D-galactose-induced aging of mice were determined. The average molecular weight of HM, MM, and LM was 58.3×10 6 , 4.68×10 6 , and 3.14×10 5 Da, respectively. All three TFPs were devoid of triple helix conformation and exhibited concentration- and molecular weight-dependent scavenging activity against radicals. The TFPs markedly relieved skin aging, effectively attenuated oxidative stress, and significantly decreased inflammation in D-galactose-induced aging mice. MM exhibited the best anti-aging effect among the TFPs. Additionally, TFPs partially restored the alterations in pH and the total content of short-chain fatty acids (SCFAs) in the colon but exhibited various impacts on the content of the individual SCFAs. These findings would provide rational guidance for a better application of TFPs in anti-aging foods and expand our understanding of the structure-function relationship of mushroom polysaccharides. |
| format | Article |
| id | doaj-art-d57c562da096455d93f15856d112e646 |
| institution | Kabale University |
| issn | 2083-6007 |
| language | English |
| publishDate | 2023-05-01 |
| publisher | Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences |
| record_format | Article |
| series | Polish Journal of Food and Nutrition Sciences |
| spelling | doaj-art-d57c562da096455d93f15856d112e6462025-02-03T06:48:52ZengInstitute of Animal Reproduction and Food Research of the Polish Academy of SciencesPolish Journal of Food and Nutrition Sciences2083-60072023-05-0173216317410.31883/pjfns/163612163612Effects of Tremella fuciformis -Derived Polysaccharides with Different Molecular Weight on D-Galactose-Induced Aging of MiceDonghui Luo0https://orcid.org/0000-0002-8584-7344Xiaofei Liu1https://orcid.org/0009-0005-6249-4194Jingjing Guan2https://orcid.org/0009-0008-6865-4162Guili Jang3https://orcid.org/0009-0009-4694-0579Yanglin Hua4https://orcid.org/0009-0008-6466-331XXinfei Zhang5https://orcid.org/0009-0000-7947-138XXiaofei Xu6https://orcid.org/0000-0001-9177-9723College of Food Science and Engineering, Guangdong Ocean University, Yangjiang 529500, PR ChinaCollege of Food Science and Engineering, Guangdong Ocean University, Yangjiang 529500, PR ChinaCollege of Food Science and Engineering, Guangdong Ocean University, Yangjiang 529500, PR ChinaCollege of Food Science and Engineering, Guangdong Ocean University, Yangjiang 529500, PR ChinaCollege of Food Science and Engineering, Guangdong Ocean University, Yangjiang 529500, PR ChinaCollege of Food Science and Engineering, South China University of Technology, Guangzhou 510640, PR ChinaCollege of Food Science and Engineering, Guangdong Ocean University, Yangjiang 529500, PR ChinaThe structure-bioactivity relationship of Tremella fuciformis polysaccharides (TFPs) in anti-aging in vivo is rarely reported. In the present study, a purified TFP, named HM, mainly composed of mannose, fucose, xylose, and glucose in a molar ratio of 4.14:0.98:0.81:0.62, was obtained from the fruiting body of T. fuciformis . Subsequently, two differentially degraded TFPs, named MM and LM, respectively, were prepared by a combined method of ultrasonic irradiation (US) and H 2 O 2 treatment. Their structural properties, scavenging activities against free radicals in vitro , and anti-aging effects on D-galactose-induced aging of mice were determined. The average molecular weight of HM, MM, and LM was 58.3×10 6 , 4.68×10 6 , and 3.14×10 5 Da, respectively. All three TFPs were devoid of triple helix conformation and exhibited concentration- and molecular weight-dependent scavenging activity against radicals. The TFPs markedly relieved skin aging, effectively attenuated oxidative stress, and significantly decreased inflammation in D-galactose-induced aging mice. MM exhibited the best anti-aging effect among the TFPs. Additionally, TFPs partially restored the alterations in pH and the total content of short-chain fatty acids (SCFAs) in the colon but exhibited various impacts on the content of the individual SCFAs. These findings would provide rational guidance for a better application of TFPs in anti-aging foods and expand our understanding of the structure-function relationship of mushroom polysaccharides.http://journal.pan.olsztyn.pl/Effects-of-Tremella-fuciformis-Derived-Polysaccharides-with-Different-Molecular-Weight,163612,0,2.htmlmushroompolysaccharide purificationanti-oxidant activityanti-aging activitystructure-function relationshipanti-oxidant enzyme |
| spellingShingle | Donghui Luo Xiaofei Liu Jingjing Guan Guili Jang Yanglin Hua Xinfei Zhang Xiaofei Xu Effects of Tremella fuciformis -Derived Polysaccharides with Different Molecular Weight on D-Galactose-Induced Aging of Mice Polish Journal of Food and Nutrition Sciences mushroom polysaccharide purification anti-oxidant activity anti-aging activity structure-function relationship anti-oxidant enzyme |
| title | Effects of Tremella fuciformis -Derived Polysaccharides with Different Molecular Weight on D-Galactose-Induced Aging of Mice |
| title_full | Effects of Tremella fuciformis -Derived Polysaccharides with Different Molecular Weight on D-Galactose-Induced Aging of Mice |
| title_fullStr | Effects of Tremella fuciformis -Derived Polysaccharides with Different Molecular Weight on D-Galactose-Induced Aging of Mice |
| title_full_unstemmed | Effects of Tremella fuciformis -Derived Polysaccharides with Different Molecular Weight on D-Galactose-Induced Aging of Mice |
| title_short | Effects of Tremella fuciformis -Derived Polysaccharides with Different Molecular Weight on D-Galactose-Induced Aging of Mice |
| title_sort | effects of tremella fuciformis derived polysaccharides with different molecular weight on d galactose induced aging of mice |
| topic | mushroom polysaccharide purification anti-oxidant activity anti-aging activity structure-function relationship anti-oxidant enzyme |
| url | http://journal.pan.olsztyn.pl/Effects-of-Tremella-fuciformis-Derived-Polysaccharides-with-Different-Molecular-Weight,163612,0,2.html |
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