Cord serum metabolome and birth weight in patients with gestational diabetes treated with metformin, insulin, or diet alone

Introduction Recent research has demonstrated the benefits of metformin treatment in gestational diabetes (GDM) on short-term pregnancy outcomes (including excessive fetal growth and pre-eclampsia), but its effects on fetal metabolism remain mostly unknown. Our aim was to study the effects of metfor...

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Main Authors: Kristiina Tertti, Tapani Rönnemaa, Outi Pellonperä, Mikael S Huhtala
Format: Article
Language:English
Published: BMJ Publishing Group 2021-03-01
Series:BMJ Open Diabetes Research & Care
Online Access:https://drc.bmj.com/content/9/1/e002022.full
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author Kristiina Tertti
Tapani Rönnemaa
Outi Pellonperä
Mikael S Huhtala
author_facet Kristiina Tertti
Tapani Rönnemaa
Outi Pellonperä
Mikael S Huhtala
author_sort Kristiina Tertti
collection DOAJ
description Introduction Recent research has demonstrated the benefits of metformin treatment in gestational diabetes (GDM) on short-term pregnancy outcomes (including excessive fetal growth and pre-eclampsia), but its effects on fetal metabolism remain mostly unknown. Our aim was to study the effects of metformin treatment compared with insulin or diet on the cord serum metabolome and also to assess how these metabolites are related to birth weight (BW) in pregnancies complicated by GDM.Research design and methods Cord serum samples were available from 113, 97, and 98 patients with GDM treated with diet, insulin, and metformin, respectively. A targeted metabolome was measured using nuclear magnetic resonance spectroscopy. The patients in the metformin and insulin groups had participated in a previous randomized trial (NCT01240785).Results Cord serum alanine was elevated in the metformin group (0.53 mmol/L) compared with the insulin (0.45 mmol/L, p<0.001) and the diet groups (0.46 mmol/L, p<0.0001). All other measured metabolites were similar between the groups. The triglyceride (TG)-to-phosphoglyceride ratio, average very low-density lipoprotein particle diameter, docosahexaenoic acid, omega-3 fatty acids (FAs), and ratios of omega-3 and monounsaturated FA to total FA were inversely related to BW. The omega-6-to-total-FA and omega-6-to-omega-3-FA ratios were positively related to BW. Cholesterol in very large and large high-density lipoprotein (HDL) was positively (p<0.01) associated with BW when adjusted for maternal prepregnancy body mass index, gestational weight gain, glycated hemoglobin, and mode of delivery.Conclusions Metformin treatment in GDM leads to an increase in cord serum alanine. The possible long-term implications of elevated neonatal alanine in this context need to be evaluated in future studies. Although previous studies have shown that metformin increased maternal TG levels, the cord serum TG levels were not affected. Cord serum HDL cholesterol and several FA variables are related to the regulation of fetal growth in GDM. Moreover, these associations seem to be independent of maternal confounding factors.Trial registration number NCT01240785.
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spelling doaj-art-d578288713414666b4b76e0951dbd20b2025-08-20T01:59:05ZengBMJ Publishing GroupBMJ Open Diabetes Research & Care2052-48972021-03-019110.1136/bmjdrc-2020-002022Cord serum metabolome and birth weight in patients with gestational diabetes treated with metformin, insulin, or diet aloneKristiina Tertti0Tapani Rönnemaa1Outi Pellonperä2Mikael S Huhtala3Obstetrics and Gynecology, University of Turku, Turku, FinlandMedicine, University of Turku, Turku, FinlandObstetrics and Gynecology, University of Turku, Turku, FinlandObstetrics and Gynecology, University of Turku, Turku, FinlandIntroduction Recent research has demonstrated the benefits of metformin treatment in gestational diabetes (GDM) on short-term pregnancy outcomes (including excessive fetal growth and pre-eclampsia), but its effects on fetal metabolism remain mostly unknown. Our aim was to study the effects of metformin treatment compared with insulin or diet on the cord serum metabolome and also to assess how these metabolites are related to birth weight (BW) in pregnancies complicated by GDM.Research design and methods Cord serum samples were available from 113, 97, and 98 patients with GDM treated with diet, insulin, and metformin, respectively. A targeted metabolome was measured using nuclear magnetic resonance spectroscopy. The patients in the metformin and insulin groups had participated in a previous randomized trial (NCT01240785).Results Cord serum alanine was elevated in the metformin group (0.53 mmol/L) compared with the insulin (0.45 mmol/L, p<0.001) and the diet groups (0.46 mmol/L, p<0.0001). All other measured metabolites were similar between the groups. The triglyceride (TG)-to-phosphoglyceride ratio, average very low-density lipoprotein particle diameter, docosahexaenoic acid, omega-3 fatty acids (FAs), and ratios of omega-3 and monounsaturated FA to total FA were inversely related to BW. The omega-6-to-total-FA and omega-6-to-omega-3-FA ratios were positively related to BW. Cholesterol in very large and large high-density lipoprotein (HDL) was positively (p<0.01) associated with BW when adjusted for maternal prepregnancy body mass index, gestational weight gain, glycated hemoglobin, and mode of delivery.Conclusions Metformin treatment in GDM leads to an increase in cord serum alanine. The possible long-term implications of elevated neonatal alanine in this context need to be evaluated in future studies. Although previous studies have shown that metformin increased maternal TG levels, the cord serum TG levels were not affected. Cord serum HDL cholesterol and several FA variables are related to the regulation of fetal growth in GDM. Moreover, these associations seem to be independent of maternal confounding factors.Trial registration number NCT01240785.https://drc.bmj.com/content/9/1/e002022.full
spellingShingle Kristiina Tertti
Tapani Rönnemaa
Outi Pellonperä
Mikael S Huhtala
Cord serum metabolome and birth weight in patients with gestational diabetes treated with metformin, insulin, or diet alone
BMJ Open Diabetes Research & Care
title Cord serum metabolome and birth weight in patients with gestational diabetes treated with metformin, insulin, or diet alone
title_full Cord serum metabolome and birth weight in patients with gestational diabetes treated with metformin, insulin, or diet alone
title_fullStr Cord serum metabolome and birth weight in patients with gestational diabetes treated with metformin, insulin, or diet alone
title_full_unstemmed Cord serum metabolome and birth weight in patients with gestational diabetes treated with metformin, insulin, or diet alone
title_short Cord serum metabolome and birth weight in patients with gestational diabetes treated with metformin, insulin, or diet alone
title_sort cord serum metabolome and birth weight in patients with gestational diabetes treated with metformin insulin or diet alone
url https://drc.bmj.com/content/9/1/e002022.full
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AT outipellonpera cordserummetabolomeandbirthweightinpatientswithgestationaldiabetestreatedwithmetformininsulinordietalone
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