Influence of Growth Regulators on Callogenesis and Somatic Embryo Development in Date Palm (Phoenix dactylifera L.) Sahelian Cultivars
This study provides a physiological analysis of somatic embryogenesis in four elite cultivars of date palms: Ahmar, Amsekhsi, Tijib, and Amaside, from the initial callogenesis to establishment and proliferation of embryogenic suspension cultures. Somatic embryos development and in vitro plants rooti...
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/837395 |
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| author | Djibril Sané Frédérique Aberlenc-Bertossi Léopold Ibrahima Djitiningo Diatta Badara Guèye Abdourahman Daher Maurice Sagna Yves Duval Alain Borgel |
| author_facet | Djibril Sané Frédérique Aberlenc-Bertossi Léopold Ibrahima Djitiningo Diatta Badara Guèye Abdourahman Daher Maurice Sagna Yves Duval Alain Borgel |
| author_sort | Djibril Sané |
| collection | DOAJ |
| description | This study provides a physiological analysis of somatic embryogenesis in four elite cultivars of date palms: Ahmar, Amsekhsi, Tijib, and Amaside, from the initial callogenesis to establishment and proliferation of embryogenic suspension cultures. Somatic embryos development and in vitro plants rooting were also studied. For each step, auxins and cytokinins concentrations were optimised. The primary callogenesis from leaf explants of seedlings appeared highly dependent on genotype. Ahmar (80%) and Amsekhsi (76%) appeared highly callogenic, whereas Tijib (10%) and Amaside (2%) produced low amounts of calluses. 2,4-Dichlorophenoxyacetic acid appeared favorable to the induction of primary callogenesis and its effect was enhanced by the addition of benzyl adenine or adenine sulfate. Secondary friable calli obtained from chopped granular calli were used to initiate embryogenic cell suspensions in media supplied with 2,4-dichlorophenoxyacetic acid. Suspension cultures showed a growth rate of fourfold after four subcultures in presence of 2,4-dichlorophenoxyacetic acid 2 mg/L. Our results showed that a seven-day transitory treatment with benzyl adenine 0,5 mg/L was necessary to optimize embryos development. Naphthalene acetic acid induced the development of primary orthogravitropic roots during embryos germination. The comparison with cytofluorometry of nuclear DNA amounts showed no significant difference in ploidy level between regenerated plants and seedlings. |
| format | Article |
| id | doaj-art-d5746ea5afa542428e16fc9a6ada2af5 |
| institution | OA Journals |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-d5746ea5afa542428e16fc9a6ada2af52025-08-20T02:19:31ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/837395837395Influence of Growth Regulators on Callogenesis and Somatic Embryo Development in Date Palm (Phoenix dactylifera L.) Sahelian CultivarsDjibril Sané0Frédérique Aberlenc-Bertossi1Léopold Ibrahima Djitiningo Diatta2Badara Guèye3Abdourahman Daher4Maurice Sagna5Yves Duval6Alain Borgel7Département de Biologie Végétale, Université Cheikh Anta Diop, BP 5005, Dakar-Fann, SenegalInstitut de Recherche pour le Développement, IRD, BP 64501, 34394 Montpellier Cedex 5, FranceDépartement de Biologie Végétale, Université Cheikh Anta Diop, BP 5005, Dakar-Fann, SenegalDépartement de Biologie Végétale, Université Cheikh Anta Diop, BP 5005, Dakar-Fann, SenegalInstitut des sciences de la vie, Centre d'Etudes et de Recherche de Djibouti (BP 486), BP 5005, Dakar-Fann, DjiboutiDépartement de Biologie Végétale, Université Cheikh Anta Diop, BP 5005, Dakar-Fann, SenegalInstitut de Recherche pour le Développement, IRD, BP 64501, 34394 Montpellier Cedex 5, FranceInstitut de Recherche pour le Développement, IRD, BP 64501, 34394 Montpellier Cedex 5, FranceThis study provides a physiological analysis of somatic embryogenesis in four elite cultivars of date palms: Ahmar, Amsekhsi, Tijib, and Amaside, from the initial callogenesis to establishment and proliferation of embryogenic suspension cultures. Somatic embryos development and in vitro plants rooting were also studied. For each step, auxins and cytokinins concentrations were optimised. The primary callogenesis from leaf explants of seedlings appeared highly dependent on genotype. Ahmar (80%) and Amsekhsi (76%) appeared highly callogenic, whereas Tijib (10%) and Amaside (2%) produced low amounts of calluses. 2,4-Dichlorophenoxyacetic acid appeared favorable to the induction of primary callogenesis and its effect was enhanced by the addition of benzyl adenine or adenine sulfate. Secondary friable calli obtained from chopped granular calli were used to initiate embryogenic cell suspensions in media supplied with 2,4-dichlorophenoxyacetic acid. Suspension cultures showed a growth rate of fourfold after four subcultures in presence of 2,4-dichlorophenoxyacetic acid 2 mg/L. Our results showed that a seven-day transitory treatment with benzyl adenine 0,5 mg/L was necessary to optimize embryos development. Naphthalene acetic acid induced the development of primary orthogravitropic roots during embryos germination. The comparison with cytofluorometry of nuclear DNA amounts showed no significant difference in ploidy level between regenerated plants and seedlings.http://dx.doi.org/10.1100/2012/837395 |
| spellingShingle | Djibril Sané Frédérique Aberlenc-Bertossi Léopold Ibrahima Djitiningo Diatta Badara Guèye Abdourahman Daher Maurice Sagna Yves Duval Alain Borgel Influence of Growth Regulators on Callogenesis and Somatic Embryo Development in Date Palm (Phoenix dactylifera L.) Sahelian Cultivars The Scientific World Journal |
| title | Influence of Growth Regulators on Callogenesis and Somatic Embryo Development in Date Palm (Phoenix dactylifera L.) Sahelian Cultivars |
| title_full | Influence of Growth Regulators on Callogenesis and Somatic Embryo Development in Date Palm (Phoenix dactylifera L.) Sahelian Cultivars |
| title_fullStr | Influence of Growth Regulators on Callogenesis and Somatic Embryo Development in Date Palm (Phoenix dactylifera L.) Sahelian Cultivars |
| title_full_unstemmed | Influence of Growth Regulators on Callogenesis and Somatic Embryo Development in Date Palm (Phoenix dactylifera L.) Sahelian Cultivars |
| title_short | Influence of Growth Regulators on Callogenesis and Somatic Embryo Development in Date Palm (Phoenix dactylifera L.) Sahelian Cultivars |
| title_sort | influence of growth regulators on callogenesis and somatic embryo development in date palm phoenix dactylifera l sahelian cultivars |
| url | http://dx.doi.org/10.1100/2012/837395 |
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