Vitamin D3 Activates Phosphatidylinositol-3-Kinase/Protein Kinase B via Insulin-Like Growth Factor-1 to Improve Testicular Function in Diabetic Rats
Objective. In diabetes mellitus, vitamin D3 deficiency affects sex hormone levels and male fertility; however, the mechanism leading to the disorder is unclear. This research was designed to investigate the mechanism of vitamin D3 deficiency and hypogonadism in diabetic rats. Our aim was to assess s...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2019/7894950 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849683912163328000 |
|---|---|
| author | Yanyan He Yang Liu Qing-Zhu Wang Feng Guo Fengjuan Huang Linlin Ji Tingting An Guijun Qin |
| author_facet | Yanyan He Yang Liu Qing-Zhu Wang Feng Guo Fengjuan Huang Linlin Ji Tingting An Guijun Qin |
| author_sort | Yanyan He |
| collection | DOAJ |
| description | Objective. In diabetes mellitus, vitamin D3 deficiency affects sex hormone levels and male fertility; however, the mechanism leading to the disorder is unclear. This research was designed to investigate the mechanism of vitamin D3 deficiency and hypogonadism in diabetic rats. Our aim was to assess serum vitamin D3 levels and the relationship among vitamin D3, insulin-like growth factor-1 (IGF-1), and testicular function. Materials and Methods. Rats with streptozotocin-induced diabetes were randomly divided into four groups and treated with different doses of vitamin D3: no vitamin D3, low (0.025 μg/kg/day), high (0.1 μg/kg/day), and high (0.1 μg/kg/day) with JB-1 (the insulin-like growth factor-1 receptor inhibitor group, 100 μg/kg/day). The groups were compared with wild-type rats, which function as the control group. Various parameters such as vitamin D3 and IGF-1 were compared between the experimental and wild-type groups, and their correlations were determined. Results. Twelve weeks of vitamin D3 supplementation improved the testosterone levels, as shown by the increase in the level of serum IGF-1 in diabetic rats. Phosphatidylinositol-3-kinase (PI3K)/protein kinase B (AKT), which was a downstream of the signaling pathway of IGF-1, was significantly increased after vitamin D3 treatment. Conclusions. The study shows that vitamin D3 may promote the expression of testosterone and improve testicular function in diabetic rats by activating PI3K/AKT via IGF-1. |
| format | Article |
| id | doaj-art-d5581ddc58a94533afbb197dbb697cd9 |
| institution | DOAJ |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-d5581ddc58a94533afbb197dbb697cd92025-08-20T03:23:38ZengWileyJournal of Diabetes Research2314-67452314-67532019-01-01201910.1155/2019/78949507894950Vitamin D3 Activates Phosphatidylinositol-3-Kinase/Protein Kinase B via Insulin-Like Growth Factor-1 to Improve Testicular Function in Diabetic RatsYanyan He0Yang Liu1Qing-Zhu Wang2Feng Guo3Fengjuan Huang4Linlin Ji5Tingting An6Guijun Qin7Division of Endocrinology, Department of Internal Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaDivision of Endocrinology, Department of Internal Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaDivision of Endocrinology, Department of Internal Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaDivision of Endocrinology, Department of Internal Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaDivision of Endocrinology, Department of Internal Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaDivision of Endocrinology, Department of Internal Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaDivision of Endocrinology, Department of Internal Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaDivision of Endocrinology, Department of Internal Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaObjective. In diabetes mellitus, vitamin D3 deficiency affects sex hormone levels and male fertility; however, the mechanism leading to the disorder is unclear. This research was designed to investigate the mechanism of vitamin D3 deficiency and hypogonadism in diabetic rats. Our aim was to assess serum vitamin D3 levels and the relationship among vitamin D3, insulin-like growth factor-1 (IGF-1), and testicular function. Materials and Methods. Rats with streptozotocin-induced diabetes were randomly divided into four groups and treated with different doses of vitamin D3: no vitamin D3, low (0.025 μg/kg/day), high (0.1 μg/kg/day), and high (0.1 μg/kg/day) with JB-1 (the insulin-like growth factor-1 receptor inhibitor group, 100 μg/kg/day). The groups were compared with wild-type rats, which function as the control group. Various parameters such as vitamin D3 and IGF-1 were compared between the experimental and wild-type groups, and their correlations were determined. Results. Twelve weeks of vitamin D3 supplementation improved the testosterone levels, as shown by the increase in the level of serum IGF-1 in diabetic rats. Phosphatidylinositol-3-kinase (PI3K)/protein kinase B (AKT), which was a downstream of the signaling pathway of IGF-1, was significantly increased after vitamin D3 treatment. Conclusions. The study shows that vitamin D3 may promote the expression of testosterone and improve testicular function in diabetic rats by activating PI3K/AKT via IGF-1.http://dx.doi.org/10.1155/2019/7894950 |
| spellingShingle | Yanyan He Yang Liu Qing-Zhu Wang Feng Guo Fengjuan Huang Linlin Ji Tingting An Guijun Qin Vitamin D3 Activates Phosphatidylinositol-3-Kinase/Protein Kinase B via Insulin-Like Growth Factor-1 to Improve Testicular Function in Diabetic Rats Journal of Diabetes Research |
| title | Vitamin D3 Activates Phosphatidylinositol-3-Kinase/Protein Kinase B via Insulin-Like Growth Factor-1 to Improve Testicular Function in Diabetic Rats |
| title_full | Vitamin D3 Activates Phosphatidylinositol-3-Kinase/Protein Kinase B via Insulin-Like Growth Factor-1 to Improve Testicular Function in Diabetic Rats |
| title_fullStr | Vitamin D3 Activates Phosphatidylinositol-3-Kinase/Protein Kinase B via Insulin-Like Growth Factor-1 to Improve Testicular Function in Diabetic Rats |
| title_full_unstemmed | Vitamin D3 Activates Phosphatidylinositol-3-Kinase/Protein Kinase B via Insulin-Like Growth Factor-1 to Improve Testicular Function in Diabetic Rats |
| title_short | Vitamin D3 Activates Phosphatidylinositol-3-Kinase/Protein Kinase B via Insulin-Like Growth Factor-1 to Improve Testicular Function in Diabetic Rats |
| title_sort | vitamin d3 activates phosphatidylinositol 3 kinase protein kinase b via insulin like growth factor 1 to improve testicular function in diabetic rats |
| url | http://dx.doi.org/10.1155/2019/7894950 |
| work_keys_str_mv | AT yanyanhe vitamind3activatesphosphatidylinositol3kinaseproteinkinasebviainsulinlikegrowthfactor1toimprovetesticularfunctionindiabeticrats AT yangliu vitamind3activatesphosphatidylinositol3kinaseproteinkinasebviainsulinlikegrowthfactor1toimprovetesticularfunctionindiabeticrats AT qingzhuwang vitamind3activatesphosphatidylinositol3kinaseproteinkinasebviainsulinlikegrowthfactor1toimprovetesticularfunctionindiabeticrats AT fengguo vitamind3activatesphosphatidylinositol3kinaseproteinkinasebviainsulinlikegrowthfactor1toimprovetesticularfunctionindiabeticrats AT fengjuanhuang vitamind3activatesphosphatidylinositol3kinaseproteinkinasebviainsulinlikegrowthfactor1toimprovetesticularfunctionindiabeticrats AT linlinji vitamind3activatesphosphatidylinositol3kinaseproteinkinasebviainsulinlikegrowthfactor1toimprovetesticularfunctionindiabeticrats AT tingtingan vitamind3activatesphosphatidylinositol3kinaseproteinkinasebviainsulinlikegrowthfactor1toimprovetesticularfunctionindiabeticrats AT guijunqin vitamind3activatesphosphatidylinositol3kinaseproteinkinasebviainsulinlikegrowthfactor1toimprovetesticularfunctionindiabeticrats |