Combined effects of prolonged temperature and hypoxia stress on growth, metabolism and physiological status of yellow catfish (Pelteobagrus fulvidraco)

Temperature and dissolved oxygen concentration are critical to fish. This study analyzed the effects of prolonged temperature and dissolved oxygen stress on growth metabolism, oxidative stress, immunity, and apoptosis in yellow catfish (Pelteobagrus fulvidraco). To this end, yellow catfish were cont...

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Main Authors: Jie Wang, Liang Li, Shasha Zhao, Man Wang, Bo Li, Hongxi Guo, Dong Han, Xiaoming Zhu, Lei Zhang
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:Aquaculture Reports
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Online Access:http://www.sciencedirect.com/science/article/pii/S2352513425002704
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author Jie Wang
Liang Li
Shasha Zhao
Man Wang
Bo Li
Hongxi Guo
Dong Han
Xiaoming Zhu
Lei Zhang
author_facet Jie Wang
Liang Li
Shasha Zhao
Man Wang
Bo Li
Hongxi Guo
Dong Han
Xiaoming Zhu
Lei Zhang
author_sort Jie Wang
collection DOAJ
description Temperature and dissolved oxygen concentration are critical to fish. This study analyzed the effects of prolonged temperature and dissolved oxygen stress on growth metabolism, oxidative stress, immunity, and apoptosis in yellow catfish (Pelteobagrus fulvidraco). To this end, yellow catfish were continuously exposed to three temperatures (15, 26, and 32 °C) and two dissolved oxygen concentrations (2.5 and 6.5 mg/L) for 28 days. Notably, unsuitable temperatures and hypoxic exposure significantly decreased growth parameters (final body weight, weight gain, and specific growth rate), decreased survival rate, and significantly decreased crude protein and crude lipid in muscle (p < 0.05). Further, the serum glucose content was reduced, lactate dehydrogenase activity and lactic acid content were increased, and the activities of key glycolysis enzymes (hexokinase, pyruvate kinase, and phosphofructokinase) were significantly elevated (p < 0.05). Notably, prolonged temperature and dissolved oxygen stress triggered oxidative stress in yellow catfish, as evidenced by a significant increase in malondialdehyde content in serum and gills (p < 0.05), elevated levels of antioxidant enzyme activity and expression (superoxide dismutase, catalase, and glutathione peroxidase), and damaged gills and head kidney tissues. In addition, the expression of immuno-inflammatory factors and apoptotic genes (c3-like, tnfaip2, il-1β, caspase-3 and caspase-9) was significantly increased (p < 0.05) in the head kidney of the yellow catfish. Our findings showed that combined long-term temperature and dissolved oxygen stress have complex effects on fish, with the growth performance of yellow catfish at 32 °C more susceptible to hypoxia stress than at 15 °C. Collectively, these findings elucidate the response mechanism of fish to long-term temperature and dissolved oxygen stress and provide a theoretical resource to guide the healthy aquaculture of aquatic products.
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spelling doaj-art-d54e245e34364a5fa28bf4a6e7bbc1942025-08-20T03:45:10ZengElsevierAquaculture Reports2352-51342025-09-014310288410.1016/j.aqrep.2025.102884Combined effects of prolonged temperature and hypoxia stress on growth, metabolism and physiological status of yellow catfish (Pelteobagrus fulvidraco)Jie Wang0Liang Li1Shasha Zhao2Man Wang3Bo Li4Hongxi Guo5Dong Han6Xiaoming Zhu7Lei Zhang8Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; Huai’an Research Centre of Hydrobiology, Huai’an 223002, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaChangjiang Survey, Planning, Design and Research Co. Ltd., Wuhan 430010, ChinaShandong Engineering Research Center of Green and High-value Marine Fine Chemical, School of Chemical Engineering and Environment, Weifang University of Science and Technology, Weifang 262700, ChinaInstitute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; Huai’an Research Centre of Hydrobiology, Huai’an 223002, ChinaWuhan Academy of Agricultural Sciences, Wuhan 430065, ChinaWuhan Academy of Agricultural Sciences, Wuhan 430065, ChinaInstitute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaInstitute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaInstitute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; Huai’an Research Centre of Hydrobiology, Huai’an 223002, China; University of Chinese Academy of Sciences, Beijing 100049, China; Corresponding author at: Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.Temperature and dissolved oxygen concentration are critical to fish. This study analyzed the effects of prolonged temperature and dissolved oxygen stress on growth metabolism, oxidative stress, immunity, and apoptosis in yellow catfish (Pelteobagrus fulvidraco). To this end, yellow catfish were continuously exposed to three temperatures (15, 26, and 32 °C) and two dissolved oxygen concentrations (2.5 and 6.5 mg/L) for 28 days. Notably, unsuitable temperatures and hypoxic exposure significantly decreased growth parameters (final body weight, weight gain, and specific growth rate), decreased survival rate, and significantly decreased crude protein and crude lipid in muscle (p < 0.05). Further, the serum glucose content was reduced, lactate dehydrogenase activity and lactic acid content were increased, and the activities of key glycolysis enzymes (hexokinase, pyruvate kinase, and phosphofructokinase) were significantly elevated (p < 0.05). Notably, prolonged temperature and dissolved oxygen stress triggered oxidative stress in yellow catfish, as evidenced by a significant increase in malondialdehyde content in serum and gills (p < 0.05), elevated levels of antioxidant enzyme activity and expression (superoxide dismutase, catalase, and glutathione peroxidase), and damaged gills and head kidney tissues. In addition, the expression of immuno-inflammatory factors and apoptotic genes (c3-like, tnfaip2, il-1β, caspase-3 and caspase-9) was significantly increased (p < 0.05) in the head kidney of the yellow catfish. Our findings showed that combined long-term temperature and dissolved oxygen stress have complex effects on fish, with the growth performance of yellow catfish at 32 °C more susceptible to hypoxia stress than at 15 °C. Collectively, these findings elucidate the response mechanism of fish to long-term temperature and dissolved oxygen stress and provide a theoretical resource to guide the healthy aquaculture of aquatic products.http://www.sciencedirect.com/science/article/pii/S2352513425002704temperaturedissolved oxygengrowth metabolismoxidative stressimmunityPelteobagrus fulvidraco
spellingShingle Jie Wang
Liang Li
Shasha Zhao
Man Wang
Bo Li
Hongxi Guo
Dong Han
Xiaoming Zhu
Lei Zhang
Combined effects of prolonged temperature and hypoxia stress on growth, metabolism and physiological status of yellow catfish (Pelteobagrus fulvidraco)
Aquaculture Reports
temperature
dissolved oxygen
growth metabolism
oxidative stress
immunity
Pelteobagrus fulvidraco
title Combined effects of prolonged temperature and hypoxia stress on growth, metabolism and physiological status of yellow catfish (Pelteobagrus fulvidraco)
title_full Combined effects of prolonged temperature and hypoxia stress on growth, metabolism and physiological status of yellow catfish (Pelteobagrus fulvidraco)
title_fullStr Combined effects of prolonged temperature and hypoxia stress on growth, metabolism and physiological status of yellow catfish (Pelteobagrus fulvidraco)
title_full_unstemmed Combined effects of prolonged temperature and hypoxia stress on growth, metabolism and physiological status of yellow catfish (Pelteobagrus fulvidraco)
title_short Combined effects of prolonged temperature and hypoxia stress on growth, metabolism and physiological status of yellow catfish (Pelteobagrus fulvidraco)
title_sort combined effects of prolonged temperature and hypoxia stress on growth metabolism and physiological status of yellow catfish pelteobagrus fulvidraco
topic temperature
dissolved oxygen
growth metabolism
oxidative stress
immunity
Pelteobagrus fulvidraco
url http://www.sciencedirect.com/science/article/pii/S2352513425002704
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