Preparation, characterization and in vitro evaluation of phosphate-doped bioactive glass nanoparticles as promising candidates for therapeutic applications

Abstract This research focuses on synthesizing bioactive glass nanoparticles with different phosphate concentrations to evaluate their physicochemical characteristics and explore their biomedical potential. The nanoparticles were fabricated by melting a mixture of silica, calcium oxide, sodium oxide...

Full description

Saved in:
Bibliographic Details
Main Authors: Wael M. Aboulthana, Sahar M. Mousa, Gehan T. El-Bassyouni, Esmat M. A. Hamzawy, Amal G. Hussien, M. Eltohamy
Format: Article
Language:English
Published: BMC 2025-06-01
Series:BMC Chemistry
Subjects:
Online Access:https://doi.org/10.1186/s13065-025-01543-w
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849686217130508288
author Wael M. Aboulthana
Sahar M. Mousa
Gehan T. El-Bassyouni
Esmat M. A. Hamzawy
Amal G. Hussien
M. Eltohamy
author_facet Wael M. Aboulthana
Sahar M. Mousa
Gehan T. El-Bassyouni
Esmat M. A. Hamzawy
Amal G. Hussien
M. Eltohamy
author_sort Wael M. Aboulthana
collection DOAJ
description Abstract This research focuses on synthesizing bioactive glass nanoparticles with different phosphate concentrations to evaluate their physicochemical characteristics and explore their biomedical potential. The nanoparticles were fabricated by melting a mixture of silica, calcium oxide, sodium oxide, and ammonium dihydrogen phosphate at 1450 °C, followed by ball milling to obtain the optimal particle size distribution. The study systematically investigated the effect of phosphate content on the nanoparticles’ structural properties and bioactive performance. Advanced characterization methods; such as XRD, FTIR, DTA, and FE-SEM–EDX were used to examine structural stability and phosphate-induced surface alterations. In vitro assays revealed dose-dependent antioxidant, anti-diabetic, anti-Alzheimer’s, anti-arthritic, and anti-inflammatory activities, with higher phosphate content enhancing bioactivity. Results demonstrated enhanced radical scavenging and enzyme inhibition in samples with higher phosphate levels. These findings suggest that phosphate-doped bioactive glass nanoparticles hold significant promise for therapeutic applications. The research advances the understanding of how synthesis parameters influence bioactivity, providing valuable insights for designing next-generation biomedical materials.
format Article
id doaj-art-d54d7a50ce1c4c8b899b60f89e49772a
institution DOAJ
issn 2661-801X
language English
publishDate 2025-06-01
publisher BMC
record_format Article
series BMC Chemistry
spelling doaj-art-d54d7a50ce1c4c8b899b60f89e49772a2025-08-20T03:22:46ZengBMCBMC Chemistry2661-801X2025-06-0119111710.1186/s13065-025-01543-wPreparation, characterization and in vitro evaluation of phosphate-doped bioactive glass nanoparticles as promising candidates for therapeutic applicationsWael M. Aboulthana0Sahar M. Mousa1Gehan T. El-Bassyouni2Esmat M. A. Hamzawy3Amal G. Hussien4M. Eltohamy5Biochemistry Department, Biotechnology Research Institute, National Research CentreInorganic Chemistry Department, Advanced Materials Technology and Mineral Resources Research Institute, National Research CentreRefractories, Ceramics and Building Materials Department, Advanced Materials Technology and Mineral Resources Research Institute, National Research CentreGlass Research Department, Advanced Materials Technology and Mineral Resources Research Institute, National Research CentreBiochemistry Department, Biotechnology Research Institute, National Research CentreGlass Research Department, Advanced Materials Technology and Mineral Resources Research Institute, National Research CentreAbstract This research focuses on synthesizing bioactive glass nanoparticles with different phosphate concentrations to evaluate their physicochemical characteristics and explore their biomedical potential. The nanoparticles were fabricated by melting a mixture of silica, calcium oxide, sodium oxide, and ammonium dihydrogen phosphate at 1450 °C, followed by ball milling to obtain the optimal particle size distribution. The study systematically investigated the effect of phosphate content on the nanoparticles’ structural properties and bioactive performance. Advanced characterization methods; such as XRD, FTIR, DTA, and FE-SEM–EDX were used to examine structural stability and phosphate-induced surface alterations. In vitro assays revealed dose-dependent antioxidant, anti-diabetic, anti-Alzheimer’s, anti-arthritic, and anti-inflammatory activities, with higher phosphate content enhancing bioactivity. Results demonstrated enhanced radical scavenging and enzyme inhibition in samples with higher phosphate levels. These findings suggest that phosphate-doped bioactive glass nanoparticles hold significant promise for therapeutic applications. The research advances the understanding of how synthesis parameters influence bioactivity, providing valuable insights for designing next-generation biomedical materials.https://doi.org/10.1186/s13065-025-01543-wAntioxidant and anti-inflammatory effectsBioactive glass nanoparticlesBiomedical applicationsIn vitro bioactivityPhosphate doping
spellingShingle Wael M. Aboulthana
Sahar M. Mousa
Gehan T. El-Bassyouni
Esmat M. A. Hamzawy
Amal G. Hussien
M. Eltohamy
Preparation, characterization and in vitro evaluation of phosphate-doped bioactive glass nanoparticles as promising candidates for therapeutic applications
BMC Chemistry
Antioxidant and anti-inflammatory effects
Bioactive glass nanoparticles
Biomedical applications
In vitro bioactivity
Phosphate doping
title Preparation, characterization and in vitro evaluation of phosphate-doped bioactive glass nanoparticles as promising candidates for therapeutic applications
title_full Preparation, characterization and in vitro evaluation of phosphate-doped bioactive glass nanoparticles as promising candidates for therapeutic applications
title_fullStr Preparation, characterization and in vitro evaluation of phosphate-doped bioactive glass nanoparticles as promising candidates for therapeutic applications
title_full_unstemmed Preparation, characterization and in vitro evaluation of phosphate-doped bioactive glass nanoparticles as promising candidates for therapeutic applications
title_short Preparation, characterization and in vitro evaluation of phosphate-doped bioactive glass nanoparticles as promising candidates for therapeutic applications
title_sort preparation characterization and in vitro evaluation of phosphate doped bioactive glass nanoparticles as promising candidates for therapeutic applications
topic Antioxidant and anti-inflammatory effects
Bioactive glass nanoparticles
Biomedical applications
In vitro bioactivity
Phosphate doping
url https://doi.org/10.1186/s13065-025-01543-w
work_keys_str_mv AT waelmaboulthana preparationcharacterizationandinvitroevaluationofphosphatedopedbioactiveglassnanoparticlesaspromisingcandidatesfortherapeuticapplications
AT saharmmousa preparationcharacterizationandinvitroevaluationofphosphatedopedbioactiveglassnanoparticlesaspromisingcandidatesfortherapeuticapplications
AT gehantelbassyouni preparationcharacterizationandinvitroevaluationofphosphatedopedbioactiveglassnanoparticlesaspromisingcandidatesfortherapeuticapplications
AT esmatmahamzawy preparationcharacterizationandinvitroevaluationofphosphatedopedbioactiveglassnanoparticlesaspromisingcandidatesfortherapeuticapplications
AT amalghussien preparationcharacterizationandinvitroevaluationofphosphatedopedbioactiveglassnanoparticlesaspromisingcandidatesfortherapeuticapplications
AT meltohamy preparationcharacterizationandinvitroevaluationofphosphatedopedbioactiveglassnanoparticlesaspromisingcandidatesfortherapeuticapplications