Multi-omics analysis of SFTS virus infection in Rhipicephalus microplus cells reveals antiviral tick factors
Abstract The increasing prevalence of tick-borne arboviral infections worldwide necessitates advanced control strategies, particularly those targeting vectors, to mitigate the disease burden. However, the cellular interactions between arboviruses and ticks, especially for negative-strand RNA viruses...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-05-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-59565-w |
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| author | Marine J. Petit Charlotte Flory Quan Gu Mazigh Fares Douglas Lamont Alan Score Kelsey Davies Lesley Bell-Sakyi Pietro Scaturro Benjamin Brennan Alain Kohl |
| author_facet | Marine J. Petit Charlotte Flory Quan Gu Mazigh Fares Douglas Lamont Alan Score Kelsey Davies Lesley Bell-Sakyi Pietro Scaturro Benjamin Brennan Alain Kohl |
| author_sort | Marine J. Petit |
| collection | DOAJ |
| description | Abstract The increasing prevalence of tick-borne arboviral infections worldwide necessitates advanced control strategies, particularly those targeting vectors, to mitigate the disease burden. However, the cellular interactions between arboviruses and ticks, especially for negative-strand RNA viruses, remain largely unexplored. Here, we employ a proteomics informed by transcriptomics approach to elucidate the cellular response of the Rhipicephalus microplus-derived BME/CTVM6 cell line to severe fever with thrombocytopenia syndrome virus (SFTSV) infection. We generate the de novo transcriptomes and proteomes of SFTSV- and mock-infected tick cells, identifying key host responses and regulatory pathways. Additionally, interactome analysis of the viral nucleoprotein (N) integrated host responses with viral replication and dsRNA-mediated gene silencing screen reveals two anti-SFTSV effectors: the N interacting RNA helicases DHX9 and UPF1. Collectively, our results provide insights into the antiviral responses of R. microplus vector cells and highlight critical SFTSV restriction factors, while enriching transcriptomic and proteomic resources for future research. |
| format | Article |
| id | doaj-art-d54657c9d0b9484d83aa06b082ae6191 |
| institution | DOAJ |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-d54657c9d0b9484d83aa06b082ae61912025-08-20T03:08:43ZengNature PortfolioNature Communications2041-17232025-05-0116111610.1038/s41467-025-59565-wMulti-omics analysis of SFTS virus infection in Rhipicephalus microplus cells reveals antiviral tick factorsMarine J. Petit0Charlotte Flory1Quan Gu2Mazigh Fares3Douglas Lamont4Alan Score5Kelsey Davies6Lesley Bell-Sakyi7Pietro Scaturro8Benjamin Brennan9Alain Kohl10MRC-University of Glasgow Centre for Virus ResearchLeibniz Institute of VirologyMRC-University of Glasgow Centre for Virus ResearchMRC-University of Glasgow Centre for Virus ResearchFingerprints Proteomics Facility, School of Life Science, University of DundeeFingerprints Proteomics Facility, School of Life Science, University of DundeeMRC-University of Glasgow Centre for Virus ResearchDepartment of Infection Biology and Microbiomes, Institute of Infection, Veterinary and Ecological Sciences, University of LiverpoolLeibniz Institute of VirologyMRC-University of Glasgow Centre for Virus ResearchMRC-University of Glasgow Centre for Virus ResearchAbstract The increasing prevalence of tick-borne arboviral infections worldwide necessitates advanced control strategies, particularly those targeting vectors, to mitigate the disease burden. However, the cellular interactions between arboviruses and ticks, especially for negative-strand RNA viruses, remain largely unexplored. Here, we employ a proteomics informed by transcriptomics approach to elucidate the cellular response of the Rhipicephalus microplus-derived BME/CTVM6 cell line to severe fever with thrombocytopenia syndrome virus (SFTSV) infection. We generate the de novo transcriptomes and proteomes of SFTSV- and mock-infected tick cells, identifying key host responses and regulatory pathways. Additionally, interactome analysis of the viral nucleoprotein (N) integrated host responses with viral replication and dsRNA-mediated gene silencing screen reveals two anti-SFTSV effectors: the N interacting RNA helicases DHX9 and UPF1. Collectively, our results provide insights into the antiviral responses of R. microplus vector cells and highlight critical SFTSV restriction factors, while enriching transcriptomic and proteomic resources for future research.https://doi.org/10.1038/s41467-025-59565-w |
| spellingShingle | Marine J. Petit Charlotte Flory Quan Gu Mazigh Fares Douglas Lamont Alan Score Kelsey Davies Lesley Bell-Sakyi Pietro Scaturro Benjamin Brennan Alain Kohl Multi-omics analysis of SFTS virus infection in Rhipicephalus microplus cells reveals antiviral tick factors Nature Communications |
| title | Multi-omics analysis of SFTS virus infection in Rhipicephalus microplus cells reveals antiviral tick factors |
| title_full | Multi-omics analysis of SFTS virus infection in Rhipicephalus microplus cells reveals antiviral tick factors |
| title_fullStr | Multi-omics analysis of SFTS virus infection in Rhipicephalus microplus cells reveals antiviral tick factors |
| title_full_unstemmed | Multi-omics analysis of SFTS virus infection in Rhipicephalus microplus cells reveals antiviral tick factors |
| title_short | Multi-omics analysis of SFTS virus infection in Rhipicephalus microplus cells reveals antiviral tick factors |
| title_sort | multi omics analysis of sfts virus infection in rhipicephalus microplus cells reveals antiviral tick factors |
| url | https://doi.org/10.1038/s41467-025-59565-w |
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