Exploring extracellular vesicles metrics in diabetic retinopathy vitreous, aqueous, and plasma
Purpose: Extracellular vesicles (EVs) were obtained from ocular samples and plasma of diabetic retinopathy (DR) patients who were having cataract and/or vitrectomy surgeries. Methods: Aqueous, vitreous, and plasma were collected from six patients with proliferative DR (PDR), also aqueous and plasma...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2025-05-01
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| Series: | The Pan-American Journal of Ophthalmology |
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| Online Access: | https://journals.lww.com/10.4103/pajo.pajo_54_24 |
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| author | Kleber S. Ribeiro Carmen Luz Pessuti Heloisa Nascimento Rubens Jr Belfort Alessandra G. Commodaro Ana Claudia T. Torrecilhas |
| author_facet | Kleber S. Ribeiro Carmen Luz Pessuti Heloisa Nascimento Rubens Jr Belfort Alessandra G. Commodaro Ana Claudia T. Torrecilhas |
| author_sort | Kleber S. Ribeiro |
| collection | DOAJ |
| description | Purpose:
Extracellular vesicles (EVs) were obtained from ocular samples and plasma of diabetic retinopathy (DR) patients who were having cataract and/or vitrectomy surgeries.
Methods:
Aqueous, vitreous, and plasma were collected from six patients with proliferative DR (PDR), also aqueous and plasma were collected from seven patients with non-PDR (NPDR), and seven patients with cataract (control group). EVs were isolated using the membrane affinity column method. Nanoparticle tracking analysis technique was used to measure the size and concentration of particles.
Results:
The mean size of EVs in aqueous was smaller in the PDR group comparing with the other two groups (P < 0.05). Besides, EVs concentration in plasma was higher in the PDR group than in the NPDR group (P < 0.05).
Conclusion:
We successfully isolated and measured EVs in ocular samples and plasma from DR patients. Further studies must be conducted to identify protein and molecular contents to better understand the role of particles in the pathophysiology of DR and their potential as a biomarker or medicinal target. |
| format | Article |
| id | doaj-art-d5410c672bef47c3bc0ee0c12d0c91be |
| institution | Kabale University |
| issn | 2666-4909 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | The Pan-American Journal of Ophthalmology |
| spelling | doaj-art-d5410c672bef47c3bc0ee0c12d0c91be2025-08-20T03:26:38ZengWolters Kluwer Medknow PublicationsThe Pan-American Journal of Ophthalmology2666-49092025-05-017115515510.4103/pajo.pajo_54_24Exploring extracellular vesicles metrics in diabetic retinopathy vitreous, aqueous, and plasmaKleber S. RibeiroCarmen Luz PessutiHeloisa NascimentoRubens Jr BelfortAlessandra G. CommodaroAna Claudia T. TorrecilhasPurpose: Extracellular vesicles (EVs) were obtained from ocular samples and plasma of diabetic retinopathy (DR) patients who were having cataract and/or vitrectomy surgeries. Methods: Aqueous, vitreous, and plasma were collected from six patients with proliferative DR (PDR), also aqueous and plasma were collected from seven patients with non-PDR (NPDR), and seven patients with cataract (control group). EVs were isolated using the membrane affinity column method. Nanoparticle tracking analysis technique was used to measure the size and concentration of particles. Results: The mean size of EVs in aqueous was smaller in the PDR group comparing with the other two groups (P < 0.05). Besides, EVs concentration in plasma was higher in the PDR group than in the NPDR group (P < 0.05). Conclusion: We successfully isolated and measured EVs in ocular samples and plasma from DR patients. Further studies must be conducted to identify protein and molecular contents to better understand the role of particles in the pathophysiology of DR and their potential as a biomarker or medicinal target.https://journals.lww.com/10.4103/pajo.pajo_54_24aqueousdiabetic retinopathyextracellular vesicleplasmavitreous |
| spellingShingle | Kleber S. Ribeiro Carmen Luz Pessuti Heloisa Nascimento Rubens Jr Belfort Alessandra G. Commodaro Ana Claudia T. Torrecilhas Exploring extracellular vesicles metrics in diabetic retinopathy vitreous, aqueous, and plasma The Pan-American Journal of Ophthalmology aqueous diabetic retinopathy extracellular vesicle plasma vitreous |
| title | Exploring extracellular vesicles metrics in diabetic retinopathy vitreous, aqueous, and plasma |
| title_full | Exploring extracellular vesicles metrics in diabetic retinopathy vitreous, aqueous, and plasma |
| title_fullStr | Exploring extracellular vesicles metrics in diabetic retinopathy vitreous, aqueous, and plasma |
| title_full_unstemmed | Exploring extracellular vesicles metrics in diabetic retinopathy vitreous, aqueous, and plasma |
| title_short | Exploring extracellular vesicles metrics in diabetic retinopathy vitreous, aqueous, and plasma |
| title_sort | exploring extracellular vesicles metrics in diabetic retinopathy vitreous aqueous and plasma |
| topic | aqueous diabetic retinopathy extracellular vesicle plasma vitreous |
| url | https://journals.lww.com/10.4103/pajo.pajo_54_24 |
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