Exogenous Gibberellins and Auxins Promote Crown Bud Regeneration and Influence Endogenous Hormone Changes in Alfalfa
Alfalfa (<i>Medicago sativa</i> L.) is a globally significant forage crop with notable economic value. Gibberellins (GA<sub>3</sub>) promote dormancy breaking and early germination whereas auxins (IAA) predominantly influence bud regeneration. This study investigated the effe...
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2025-06-01
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| author | Haiyan Yue Qunce Sun Shuzhen Zhang Youping An Xianwei Peng Binghan Wen Xingyu Ge Yuxiang Wang |
| author_facet | Haiyan Yue Qunce Sun Shuzhen Zhang Youping An Xianwei Peng Binghan Wen Xingyu Ge Yuxiang Wang |
| author_sort | Haiyan Yue |
| collection | DOAJ |
| description | Alfalfa (<i>Medicago sativa</i> L.) is a globally significant forage crop with notable economic value. Gibberellins (GA<sub>3</sub>) promote dormancy breaking and early germination whereas auxins (IAA) predominantly influence bud regeneration. This study investigated the effects of exogenous gibberellins and indole acetic acid on the regeneration and biomass of crown buds in two alfalfa varieties with different dormancy levels. The experiment involved five concentrations each of gibberellins (0 mg/L, 10 mg/L, 20 mg/L, 30 mg/L, and 40 mg/L) and auxins (0 mg/L, 5 mg/L, 10 mg/L, 15 mg/L, and 20 mg/L). The results indicated that both exogenous gibberellins and auxins significantly increased the endogenous levels of these hormones in the crown buds, while decreasing abscisic acid (ABA) levels. There was also a significant increase in sugar and total nitrogen content in the buds. Treatments with exogenous gibberellins enhanced the number of crown buds and the aboveground biomass per plant, with the best results at 30 mg/L. Auxin treatments showed the largest increase in aboveground biomass per plant at 15 mg/L. In summary, 30 mg/L gibberellins or 15 mg/L auxins is recommended as the optimal spraying concentration. This research provides practical evidence for the regulation of exogenous growth regulators in alfalfa cultivation. |
| format | Article |
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| institution | OA Journals |
| issn | 2223-7747 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
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| series | Plants |
| spelling | doaj-art-d5386ee5ebf142d6a42212e17c7a06362025-08-20T02:33:11ZengMDPI AGPlants2223-77472025-06-011411169910.3390/plants14111699Exogenous Gibberellins and Auxins Promote Crown Bud Regeneration and Influence Endogenous Hormone Changes in AlfalfaHaiyan Yue0Qunce Sun1Shuzhen Zhang2Youping An3Xianwei Peng4Binghan Wen5Xingyu Ge6Yuxiang Wang7College of Grassland Science, Xinjiang Agricultural University, Urumqi 830052, ChinaCollege of Grassland Science, Xinjiang Agricultural University, Urumqi 830052, ChinaCollege of Grassland Science, Xinjiang Agricultural University, Urumqi 830052, ChinaCollege of Grassland Science, Xinjiang Agricultural University, Urumqi 830052, ChinaCollege of Grassland Science, Xinjiang Agricultural University, Urumqi 830052, ChinaCollege of Grassland Science, Xinjiang Agricultural University, Urumqi 830052, ChinaCollege of Grassland Science, Xinjiang Agricultural University, Urumqi 830052, ChinaCollege of Grassland Science, Xinjiang Agricultural University, Urumqi 830052, ChinaAlfalfa (<i>Medicago sativa</i> L.) is a globally significant forage crop with notable economic value. Gibberellins (GA<sub>3</sub>) promote dormancy breaking and early germination whereas auxins (IAA) predominantly influence bud regeneration. This study investigated the effects of exogenous gibberellins and indole acetic acid on the regeneration and biomass of crown buds in two alfalfa varieties with different dormancy levels. The experiment involved five concentrations each of gibberellins (0 mg/L, 10 mg/L, 20 mg/L, 30 mg/L, and 40 mg/L) and auxins (0 mg/L, 5 mg/L, 10 mg/L, 15 mg/L, and 20 mg/L). The results indicated that both exogenous gibberellins and auxins significantly increased the endogenous levels of these hormones in the crown buds, while decreasing abscisic acid (ABA) levels. There was also a significant increase in sugar and total nitrogen content in the buds. Treatments with exogenous gibberellins enhanced the number of crown buds and the aboveground biomass per plant, with the best results at 30 mg/L. Auxin treatments showed the largest increase in aboveground biomass per plant at 15 mg/L. In summary, 30 mg/L gibberellins or 15 mg/L auxins is recommended as the optimal spraying concentration. This research provides practical evidence for the regulation of exogenous growth regulators in alfalfa cultivation.https://www.mdpi.com/2223-7747/14/11/1699exogenous growth regulatorsalfalfacrown budsregenerationendogenous hormones |
| spellingShingle | Haiyan Yue Qunce Sun Shuzhen Zhang Youping An Xianwei Peng Binghan Wen Xingyu Ge Yuxiang Wang Exogenous Gibberellins and Auxins Promote Crown Bud Regeneration and Influence Endogenous Hormone Changes in Alfalfa Plants exogenous growth regulators alfalfa crown buds regeneration endogenous hormones |
| title | Exogenous Gibberellins and Auxins Promote Crown Bud Regeneration and Influence Endogenous Hormone Changes in Alfalfa |
| title_full | Exogenous Gibberellins and Auxins Promote Crown Bud Regeneration and Influence Endogenous Hormone Changes in Alfalfa |
| title_fullStr | Exogenous Gibberellins and Auxins Promote Crown Bud Regeneration and Influence Endogenous Hormone Changes in Alfalfa |
| title_full_unstemmed | Exogenous Gibberellins and Auxins Promote Crown Bud Regeneration and Influence Endogenous Hormone Changes in Alfalfa |
| title_short | Exogenous Gibberellins and Auxins Promote Crown Bud Regeneration and Influence Endogenous Hormone Changes in Alfalfa |
| title_sort | exogenous gibberellins and auxins promote crown bud regeneration and influence endogenous hormone changes in alfalfa |
| topic | exogenous growth regulators alfalfa crown buds regeneration endogenous hormones |
| url | https://www.mdpi.com/2223-7747/14/11/1699 |
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