Chemically Defined Conditions Mediate an Efficient Induction of Dental Pulp Pluripotent-Like Stem Cells into Hepatocyte-Like Cells
Liver diseases are major causes of morbidity and mortality. Dental pulp pluripotent-like stem cells (DPPSCs) are of a considerable promise in tissue engineering and regenerative medicine as a new source of tissue-specific cells; therefore, this study is aimed at demonstrating their ability to genera...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2021/5212852 |
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| author | Carlos Gil-Recio Sheyla Montori Saddam Al Demour Mera A. Ababneh Eduard Ferrés-Padró Carles Marti Elvira Ferrés-Amat Miguel Barajas Ashraf Al Madhoun Maher Atari |
| author_facet | Carlos Gil-Recio Sheyla Montori Saddam Al Demour Mera A. Ababneh Eduard Ferrés-Padró Carles Marti Elvira Ferrés-Amat Miguel Barajas Ashraf Al Madhoun Maher Atari |
| author_sort | Carlos Gil-Recio |
| collection | DOAJ |
| description | Liver diseases are major causes of morbidity and mortality. Dental pulp pluripotent-like stem cells (DPPSCs) are of a considerable promise in tissue engineering and regenerative medicine as a new source of tissue-specific cells; therefore, this study is aimed at demonstrating their ability to generate functional hepatocyte-like cells in vitro. Cells were differentiated on a collagen scaffold in serum-free media supplemented with growth factors and cytokines to recapitulate liver development. At day 5, the differentiated DPPSC cells expressed the endodermal markers FOXA1 and FOXA2. Then, the cells were derived into the hepatic lineage generating hepatocyte-like cells. In addition to the associated morphological changes, the cells expressed the hepatic genes HNF6 and AFP. The terminally differentiated hepatocyte-like cells expressed the liver functional proteins albumin and CYP3A4. In this study, we report an efficient serum-free protocol to differentiate DPPSCs into functional hepatocyte-like cells. Our approach promotes the use of DPPSCs as a new source of adult stem cells for prospective use in liver regenerative medicine. |
| format | Article |
| id | doaj-art-d530ec69fcd04f0fb7f344480fc2b042 |
| institution | OA Journals |
| issn | 1687-9678 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-d530ec69fcd04f0fb7f344480fc2b0422025-08-20T02:19:58ZengWileyStem Cells International1687-96782021-01-01202110.1155/2021/5212852Chemically Defined Conditions Mediate an Efficient Induction of Dental Pulp Pluripotent-Like Stem Cells into Hepatocyte-Like CellsCarlos Gil-Recio0Sheyla Montori1Saddam Al Demour2Mera A. Ababneh3Eduard Ferrés-Padró4Carles Marti5Elvira Ferrés-Amat6Miguel Barajas7Ashraf Al Madhoun8Maher Atari9Regenerative Medicine Research InstituteRegenerative Medicine Research InstituteDepartment of Special Surgery/Division of UrologyDepartment of Clinical PharmacyOral and Maxillofacial Surgery DepartmentOral and Maxillofacial Surgery DepartmentPediatric Dentistry ServiceBiochemistry and Molecular Biology DepartmentDepartment of Genetics and BioinformaticsRegenerative Medicine Research InstituteLiver diseases are major causes of morbidity and mortality. Dental pulp pluripotent-like stem cells (DPPSCs) are of a considerable promise in tissue engineering and regenerative medicine as a new source of tissue-specific cells; therefore, this study is aimed at demonstrating their ability to generate functional hepatocyte-like cells in vitro. Cells were differentiated on a collagen scaffold in serum-free media supplemented with growth factors and cytokines to recapitulate liver development. At day 5, the differentiated DPPSC cells expressed the endodermal markers FOXA1 and FOXA2. Then, the cells were derived into the hepatic lineage generating hepatocyte-like cells. In addition to the associated morphological changes, the cells expressed the hepatic genes HNF6 and AFP. The terminally differentiated hepatocyte-like cells expressed the liver functional proteins albumin and CYP3A4. In this study, we report an efficient serum-free protocol to differentiate DPPSCs into functional hepatocyte-like cells. Our approach promotes the use of DPPSCs as a new source of adult stem cells for prospective use in liver regenerative medicine.http://dx.doi.org/10.1155/2021/5212852 |
| spellingShingle | Carlos Gil-Recio Sheyla Montori Saddam Al Demour Mera A. Ababneh Eduard Ferrés-Padró Carles Marti Elvira Ferrés-Amat Miguel Barajas Ashraf Al Madhoun Maher Atari Chemically Defined Conditions Mediate an Efficient Induction of Dental Pulp Pluripotent-Like Stem Cells into Hepatocyte-Like Cells Stem Cells International |
| title | Chemically Defined Conditions Mediate an Efficient Induction of Dental Pulp Pluripotent-Like Stem Cells into Hepatocyte-Like Cells |
| title_full | Chemically Defined Conditions Mediate an Efficient Induction of Dental Pulp Pluripotent-Like Stem Cells into Hepatocyte-Like Cells |
| title_fullStr | Chemically Defined Conditions Mediate an Efficient Induction of Dental Pulp Pluripotent-Like Stem Cells into Hepatocyte-Like Cells |
| title_full_unstemmed | Chemically Defined Conditions Mediate an Efficient Induction of Dental Pulp Pluripotent-Like Stem Cells into Hepatocyte-Like Cells |
| title_short | Chemically Defined Conditions Mediate an Efficient Induction of Dental Pulp Pluripotent-Like Stem Cells into Hepatocyte-Like Cells |
| title_sort | chemically defined conditions mediate an efficient induction of dental pulp pluripotent like stem cells into hepatocyte like cells |
| url | http://dx.doi.org/10.1155/2021/5212852 |
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