Simultaneous detection of Ca2+ and diacylglycerol signaling in living cells.
Phospholipase C produces two second messengers--diacylglycerol (DAG), which remains in the membrane, and inositol triphosphate (IP(3)), which triggers the release of calcium ions (Ca(2+)) from intracellular stores. Genetically encoded sensors based on a single circularly permuted fluorescent protein...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0042791&type=printable |
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| _version_ | 1850137356057706496 |
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| author | Paul Tewson Mara Westenberg Yongxin Zhao Robert E Campbell Anne Marie Quinn Thomas E Hughes |
| author_facet | Paul Tewson Mara Westenberg Yongxin Zhao Robert E Campbell Anne Marie Quinn Thomas E Hughes |
| author_sort | Paul Tewson |
| collection | DOAJ |
| description | Phospholipase C produces two second messengers--diacylglycerol (DAG), which remains in the membrane, and inositol triphosphate (IP(3)), which triggers the release of calcium ions (Ca(2+)) from intracellular stores. Genetically encoded sensors based on a single circularly permuted fluorescent protein (FP) are robust tools for studying intracellular Ca(2+) dynamics. We have developed a robust sensor for DAG based on a circularly permuted green FP that can be co-imaged with the red fluorescent Ca(2+) sensor R-GECO for simultaneous measurement of both second messengers. |
| format | Article |
| id | doaj-art-d52cb8150fb34a2b950e85cbe4f3272c |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-d52cb8150fb34a2b950e85cbe4f3272c2025-08-20T02:30:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0178e4279110.1371/journal.pone.0042791Simultaneous detection of Ca2+ and diacylglycerol signaling in living cells.Paul TewsonMara WestenbergYongxin ZhaoRobert E CampbellAnne Marie QuinnThomas E HughesPhospholipase C produces two second messengers--diacylglycerol (DAG), which remains in the membrane, and inositol triphosphate (IP(3)), which triggers the release of calcium ions (Ca(2+)) from intracellular stores. Genetically encoded sensors based on a single circularly permuted fluorescent protein (FP) are robust tools for studying intracellular Ca(2+) dynamics. We have developed a robust sensor for DAG based on a circularly permuted green FP that can be co-imaged with the red fluorescent Ca(2+) sensor R-GECO for simultaneous measurement of both second messengers.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0042791&type=printable |
| spellingShingle | Paul Tewson Mara Westenberg Yongxin Zhao Robert E Campbell Anne Marie Quinn Thomas E Hughes Simultaneous detection of Ca2+ and diacylglycerol signaling in living cells. PLoS ONE |
| title | Simultaneous detection of Ca2+ and diacylglycerol signaling in living cells. |
| title_full | Simultaneous detection of Ca2+ and diacylglycerol signaling in living cells. |
| title_fullStr | Simultaneous detection of Ca2+ and diacylglycerol signaling in living cells. |
| title_full_unstemmed | Simultaneous detection of Ca2+ and diacylglycerol signaling in living cells. |
| title_short | Simultaneous detection of Ca2+ and diacylglycerol signaling in living cells. |
| title_sort | simultaneous detection of ca2 and diacylglycerol signaling in living cells |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0042791&type=printable |
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